EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10047

Slc5a6 solute carrier family 5 (sodium-dependent vitamin transporter), member 6 ( MGI:2660847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019690. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019690_01	euxassay_019690_02	euxassay_019690_03	euxassay_019690_04

euxassay_019690_05	euxassay_019690_06	euxassay_019690_07	euxassay_019690_08	euxassay_019690_09

euxassay_019690_10	euxassay_019690_11	euxassay_019690_12	euxassay_019690_13	euxassay_019690_14

euxassay_019690_15	euxassay_019690_16	euxassay_019690_17	euxassay_019690_18	euxassay_019690_19

euxassay_019690_20	euxassay_019690_21	euxassay_019690_22	euxassay_019690_23	euxassay_019690_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10047_wholemount_strong.wlz

10047_wholemount_moderate.wlz

10047_wholemount_weak.wlz

10047_wholemount_possible.wlz

10047_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10047_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

spotted

moderate expression: see section 11 12 13 14 15 16 17 18 weak expression: see section 09 10

axial musculature

weak

regional

weak expression: see section 06 07 21 22 23

pectoral girdle and thoracic body wall musculature

weak

regional

weak expression: see section 11 12 13 16 18 19 20

pituitary gland

moderate

spotted

moderate expression: see section 12 13 15 16

lateral ventricle choroid plexus

weak

regional

weak expression: see section 06 07 08 09 10 13 16 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

urethra of male

moderate

regional

moderate expression: see section 15 16

lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 15 16 18 19 20 21 22 weak expression: see section 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56011
Entity Detected:	Slc5a6, solute carrier family 5 (sodium-dependent vitamin transporter), member 6 ( MGI:2660847)
Sequence:	sense strand is shown >T56011 TGCCTGCCAATGTAACTGTCGCCGCTGTGACCACCGTGATGCCTTCTACCCTGTCCAAGCCCACAGGACT GCAGCACTTCTATTCCCTGTCCTATTTATGGTACAGCGCGCACAACTCCACCACAGTCATTGTAGTGGGC CTGATTGTCAGTCTGCTCACTGGGGGAATGCGGGGCCGGTCCCTGAACCCCGGCACCATTTACCCTGTGC TGCCAAAGCTCCTAGCACTCCTGCCCCTATCCTGCCAGAAGCGGCTTTGCTGGAGAAGCCACAGCCAGGA TATCCCTGTGATCCCCAACCTGTTTCCAGAGAAGATGAGGAATGGGGTGCTGCAGGACAGCACAGACAAA GAGAGGATGGCTGAAGATGGCCTCGTCCACCAGCCGTGCAGCCCTACCTACGTTGTCCAGGAGACCTCCC TGTGACCTGGGGCGTGAACTCTGCCCTCGCTGCAGACAGCAGCCGGAGGCTATTTTGTAGTGCAGAGATG ACTCACTCACTCAGTGTGTTCTACAGTGCTGAGGTGGATGACCTGGCCTACAGCAAAGGACCTTGAGCTT TAAAGTATCACCTCCATGGAAGCGGGTGCATGCAAGGGCGAGACAGTTTTTCCTCATCCCTTGCCAAGCT TCTCTTAGGATTGGTTTGCAGGGTATAGAATCACATCACACGTGGGAAAATAAATGAATACACTAAGAAA AGGGTGGCATTTGATTAAGTGACCAGGGTTCTTTGGAGTGAGTAGGAACAGCATTTCTTACAACTGCCCC AGGACCTGATTTGGCAAGACTGACTAGCTCTCTTTAGCCCTCTGGTGATTCCTTCACTATCATGTCAACC TGGCTTATCATTGTGAGCCCCTGAGGTCTTTCGGGTTCTCTGTAATACTCAAGTCCTCTCTCATTCTTAC CTACCTCGACTCTTGAGAGGGAGGCTTTGGTATCCTTGGGTCTCAGCA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGCCTGCCAATGTAACTGTC; Reverse Primer - name:unspecified, sequence:TGCTGAGACCCAAGGATACC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10046 same embryo
	EurExpress:euxassay_019690 same experiment
	MGI:4828255 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source