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EMAGE:10077

Lrdd leucine-rich and death domain containing ( MGI:1889507)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10077 EMAGE:10077 EMAGE:10077 EMAGE:10077 EMAGE:10077
"Pseudo-wholemount" of euxassay_000195. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000195_01 euxassay_000195_02 euxassay_000195_03 euxassay_000195_04
EMAGE:10077 EMAGE:10077 EMAGE:10077 EMAGE:10077 EMAGE:10077
euxassay_000195_05 euxassay_000195_06 euxassay_000195_07 euxassay_000195_08 euxassay_000195_09
EMAGE:10077 EMAGE:10077 EMAGE:10077 EMAGE:10077 EMAGE:10077
euxassay_000195_10 euxassay_000195_11 euxassay_000195_12 euxassay_000195_13 euxassay_000195_14
EMAGE:10077 EMAGE:10077 EMAGE:10077 EMAGE:10077 EMAGE:10077
euxassay_000195_15 euxassay_000195_16 euxassay_000195_17 euxassay_000195_18 euxassay_000195_19
EMAGE:10077
euxassay_000195_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10077Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10077_wholemount_strong.wlz
10077_wholemount_moderate.wlz
10077_wholemount_weak.wlz
10077_wholemount_possible.wlz
10077_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10077_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nasopharynx epithelium
strong strong
homogeneousstrong expression: see section 09 10 11 moderate expression: see section 07 08
forebrain
moderate moderate
regionalmoderate expression: see section 14
diencephalon
strong strong
regionalstrong expression: see section 02 05 06 07 08 moderate expression: see section 09 10
telencephalon
strong strong
regionalstrong expression: see section 02 03 04 05 06 08 16 17 moderate expression: see section 10 11 weak expression: see section 15
hindbrain
strong strong
regionalstrong expression: see section 13
nasal cavity respiratory epithelium
strong strong
homogeneousstrong expression: see section 04 05 06 07 08 10 11 13 14 15 18
liver
weak weak
homogeneousweak expression: see section 01 02 03 04 05 06 08 09 10 11 12 13 14 15 16 17 18 19 20
urinary system
weak weak
homogeneousweak expression: see section 07
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1492
Entity Detected:Lrdd, leucine-rich and death domain containing ( MGI:1889507)
Sequence:sense strand is shown

>T1492
TTTTTTTTTTTTTTCTGTCTCACACACACAATAAATAGCTTTTATTTTATTTGTATGGATGTTTTGCCTG
CATACTTGTCTGCACACATGTGTGCCTGGTGTCTGTGGGGGCCAGAGGAAGGGGCTGACCCTTCAGAACT
GGAGTTACAGATTGTCATGAGCCATCAGGTAGGTAGAGGAAACAAACTAGGTTCCCTGAAGGAGCAGCTA
GTGTTGTTAACTACTGAGCCATCTCTCCCTCCAGGCCTTCTCTGCTCCCATCTTAGCAGAAGGGCCTGGC
ATGGTAGCAAGTATGGGCAACCTGCTCTCTATCCTTTATGTAAAACAATCTCTGAGATGTACTCACTGTG
AGTTGGAGGCTAGCTTGGGCTAGACAGTGAGTGGGTTTTTGTATGCTGGGGCACTGGAGCACACGGGTGA
GGTAGAAGGATAGGGCTCTGCCCACTGGTCGAGTCAAGTTTAAAGCTATCTTAATTAAGTCTGTAAGCCT
AGG
Notes:The probe template was PCR amplified from IMAGE:537684 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:537684 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10078 same embryo
 EurExpress:euxassay_000195 same experiment
 MGI:4825975 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS