EMAGE mouse gene expression spatial database

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EMAGE:10142

Pdcd2 programmed cell death 2 ( MGI:104643)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000153. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000153_01	euxassay_000153_02	euxassay_000153_03	euxassay_000153_04

euxassay_000153_05	euxassay_000153_06	euxassay_000153_07	euxassay_000153_08	euxassay_000153_09

euxassay_000153_10	euxassay_000153_11	euxassay_000153_12	euxassay_000153_13	euxassay_000153_14

euxassay_000153_15	euxassay_000153_16	euxassay_000153_17	euxassay_000153_18	euxassay_000153_19

euxassay_000153_20	euxassay_000153_21	euxassay_000153_22	euxassay_000153_23	euxassay_000153_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10142_wholemount_strong.wlz

10142_wholemount_moderate.wlz

10142_wholemount_weak.wlz

10142_wholemount_possible.wlz

10142_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10142_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cranial ganglion

strong

homogeneous

strong expression: see section 19 20

facial vii ganglion

strong

homogeneous

strong expression: see section 20

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 20 moderate expression: see section 09

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22 moderate expression: see section 09

vagus x ganglion

strong

homogeneous

strong expression: see section 07 08 17 18 19 20 moderate expression: see section 09 10 11

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 07 18

cranial nerve

moderate

homogeneous

moderate expression: see section 11

dorsal root ganglion

strong

homogeneous

strong expression: see section 17 18 19 moderate expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1697
Entity Detected:	Pdcd2, programmed cell death 2 ( MGI:104643)
Sequence:	sense strand is shown >T1697 TGGCCTCGAGCCAGATTCGGAACGAGGTTTTTTTTTTAGTTTTTATTTAAGTTTTTATTTTATATCTTTT GCAAGAATTTAACCCCTTTTAAGGTGTATCTGTCACATCCTGCTTCCACACAAATTCTTCTGTGTAGCCG CTACCCAGGCTACAGCTCTCAGCACAGGTGAAGACAGCCAAGACACCCCAGTCGATGCTTCTGCCGAGTC TGTCTGCCTTCAGGTGGTTCAACAGCTGAGGCATGACCTGGAATTCAAATATTCTCTTGGCACCACACGG GCAATCTGGAATATCTTTTTCTTGAGGAATATTTTCACCAGAAATCCAGATGGGTTTAATCCCTCTTCCA TACCTGAGAATCTGCTCTGGTTCAAGGGCTATTTTAGATTTAAACTTTTGGAATATGTGATCTTCCTTGG ATTCGTGCTTTGCCATGGAATCTAGTTCTTCCTCAGGAATTCCCCCCATGCTTCCTGTAACTTCAGAATA ATCCTCCATTTCCACAACCTCAGGCAAAATTTCATCTTCTGTTTCTGTTACAATTTCAAATTCTGGAA
Notes:	The probe template was PCR amplified from IMAGE:476765 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:476765 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10144 same embryo
	EMAGE:10141 same embryo
	EMAGE:10140 same embryo
	EMAGE:10143 same embryo
	EMAGE:10145 same embryo
	EurExpress:euxassay_000153 same experiment
	MGI:4827112 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source