EMAGE mouse gene expression spatial database

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EMAGE:10184

Tubgcp2 tubulin, gamma complex associated protein 2 ( MGI:1921487)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000101. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000101_01	euxassay_000101_02	euxassay_000101_03	euxassay_000101_04

euxassay_000101_05	euxassay_000101_06	euxassay_000101_07	euxassay_000101_08	euxassay_000101_09

euxassay_000101_10	euxassay_000101_11	euxassay_000101_12	euxassay_000101_13	euxassay_000101_14

euxassay_000101_15	euxassay_000101_16	euxassay_000101_17	euxassay_000101_18	euxassay_000101_19

euxassay_000101_20	euxassay_000101_21	euxassay_000101_22	euxassay_000101_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10184_wholemount_strong.wlz

10184_wholemount_moderate.wlz

10184_wholemount_weak.wlz

10184_wholemount_possible.wlz

10184_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10184_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

not examined

other

not examined expression: see section 01 10

not examined

other

not examined expression: see section 01

cerebral cortex

moderate

homogeneous

moderate expression: see section 17 weak expression: see section 06 07 08 09 10 11 15 16 18 19 20 21 22

cerebral cortex ventricular layer

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2210
Entity Detected:	Tubgcp2, tubulin, gamma complex associated protein 2 ( MGI:1921487)
Sequence:	sense strand is shown >T2210 TGGCCTCGAGNCAGATTCGGACGAGGGCACGAGGGAGAAAGCGTTCAATTATGCCAGCAAAGTGCTGCTG GACTTCCTCATGGAGGAGAAGGAGCTGGTGGCCCACCTGAGGTCCATCAAGCGCTACTTCCTGATGGACC AGGGGGACTTCTTCGTGCATTTCATGGACCTGACTGAGGAGGAGCTCAGGAAGCCAGTGGAGGACATCAT ACTCACACGCCTGGAGGCCCTGTTGGAGCTGGCCCTGCGAATGAGCACTGCCAACACTGACCCCTTCAAA GACGACCTCAAGATTGAACTGATGCCTCACGACCTAATCACTCAGCTTTTGCGGGTTCTGGCCATCGAGA CCAAGCAGGAAAAGGCAATGACCCATGCTGACCCCACGGAGCTCACACTGAGTGGTCTGGAGGCCTTCTC CTTTGACTACATGGTCAAATGGCCCCTGTCACTGATTATTAACAGGAAGGCACTCACCCGCTACCAGATG CTCTTCCGACACATGTTCTACTGCAAGCACGTGGAGCGGCAGCTCTGCAGT
Notes:	The probe template was PCR amplified from IMAGE:947602 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:947602 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10185 same embryo
	EMAGE:10183 same embryo
	EMAGE:10182 same embryo
	EurExpress:euxassay_000101 same experiment
	MGI:4829018 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source