EMAGE mouse gene expression spatial database

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EMAGE:10214

Ppp1r1b protein phosphatase 1, regulatory (inhibitor) subunit 1B ( MGI:94860)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000095. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000095_01	euxassay_000095_02	euxassay_000095_03	euxassay_000095_04

euxassay_000095_05	euxassay_000095_06	euxassay_000095_07	euxassay_000095_08	euxassay_000095_09

euxassay_000095_10	euxassay_000095_11	euxassay_000095_12	euxassay_000095_13	euxassay_000095_14

euxassay_000095_15	euxassay_000095_16	euxassay_000095_17	euxassay_000095_18	euxassay_000095_19

euxassay_000095_20	euxassay_000095_21	euxassay_000095_22	euxassay_000095_23	euxassay_000095_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10214_wholemount_strong.wlz

10214_wholemount_moderate.wlz

10214_wholemount_weak.wlz

10214_wholemount_possible.wlz

10214_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10214_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nucleus pulposus

weak

regional

weak expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2205
Entity Detected:	Ppp1r1b, protein phosphatase 1, regulatory (inhibitor) subunit 1B ( MGI:94860)
Sequence:	sense strand is shown >T2205 TGGCCTCGAGGCAGATTCGGATCCTTCCTGTCTTTCCCTGCTAGGGCTGCGGCTTCTGACTTTTAGAAGA CTAAGGCTGGTCTCTGTTTGCTTTGGCTGAAGCCCAGAGTCCCCAATGCCTTTCCCTGCCAGTCATTCTT CTATTTACCCGGTGGGAGTAGGGAGGTGAGAGAGCCCACACTGGAAACCCCAGAAGGCCATAAACAACCA TTTATTCTCCACAAGTCCCCTCCCTCTCCAGATTCTCTCCCCTGGGCACAGGAGACCCACAGGGCAGGTC CTAAGATCTGGGGAAAGGAGGTACTGAGAGCCTACAGGTGCCCTGAGATCTCCTCCTTCCCCTACCAGTG GTGGGCGTTGAGTCCCCAACCCTCTCATAGACTTCAACCAGGGCTCAGGTCTCACGCATTCTTTGCAGAG CCTCAACATGTCCCTCCCTTAGTATCCCATTCCTGCTGGGTGCCTGCAAGATGGACTGGCAGAGGCTGCT TGCTGCGTTTTGTTTGTGCTTTGGTGCCAGGAATGCACTTACTAT
Notes:	The probe template was PCR amplified from IMAGE:921764 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:921764 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10215 same embryo
	EurExpress:euxassay_000095 same experiment
	MGI:4827373 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source