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EMAGE:10343

Efna4 ephrin A4 ( MGI:106643)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10343
euxassay_018951_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10343Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10343_wholemount_weak.wlz
10343_wholemount_possible.wlz
10343_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10343_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50377
Entity Detected:Efna4, ephrin A4 ( MGI:106643)
Sequence:sense strand is shown

>T50377
AACTCCTCTAACCCCAGGTTGCTCCGAGGAGATGCCGTGGTGGAGCTGGGCTTCAACGATTACCTAGACA
TCTTCTGCCCACATTATGAAAGCCCAGGGCCCCCAGAAGGCCCGGAAACCTTTGCATTATACATGGTGGA
CTGGTCAGGCTACGAGGCCTGCACGGCAGAGGGGGCAAATGCCTTCCAGCGCTGGAATTGCTCGATGCCT
TTTGCCCCTTTCAGCCCTGTTCGATTCTCAGAAAAGATTCAGCGCTACACACCCTTCCCGCTGGGCTTTG
AGTTCTTGCCTGGAGAGACTTACTACTACATCTCGGTGCCGACTCCGGAGAGTCCTGGCCGGTGCCTGAG
ACTCCAGGTGTCTGTCTGCTGCAAGGAGAGCGGGTCATCACATGAGTCAGCTCATCCTGTTGGGAGTCCT
GGAGAAAGCGGTACGTCCGGGTGGCGGGGAGGACACGCGCCCAGCCCCCTGTGTCTCTTGCTGCTGCTGC
TGCTCCCAATCCTCCGTCTCCTGAGAGTTCTGTGAGCCAAGCACACCTTCCAAGTCACCCCAAGGAACCG
CAGCCCTTGTCAAGAGCCTCTGTGGAAGGGGTTCCTGCCACCATACAGCGATGCCAAACCAGGCAGACAG
GATGAAAC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AACTCCTCTAACCCCAGGTTGCTC; Reverse Primer - name:unspecified, sequence:GTTTCATCCTGTCTGCCTGGTTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10339 same embryo
 EMAGE:10340 same embryo
 EMAGE:10342 same embryo
 EMAGE:10344 same embryo
 EMAGE:10341 same embryo
 EurExpress:euxassay_018951 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS