EMAGE mouse gene expression spatial database

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EMAGE:10379

S100a9 S100 calcium binding protein A9 (calgranulin B) ( MGI:1338947)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000521. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000521_01	euxassay_000521_02	euxassay_000521_03	euxassay_000521_04

euxassay_000521_05	euxassay_000521_06	euxassay_000521_07	euxassay_000521_08	euxassay_000521_09

euxassay_000521_10	euxassay_000521_11	euxassay_000521_12	euxassay_000521_13	euxassay_000521_14

euxassay_000521_15	euxassay_000521_16	euxassay_000521_17	euxassay_000521_18	euxassay_000521_19

euxassay_000521_20	euxassay_000521_21	euxassay_000521_22	euxassay_000521_23	euxassay_000521_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10379_wholemount_strong.wlz

10379_wholemount_moderate.wlz

10379_wholemount_weak.wlz

10379_wholemount_possible.wlz

10379_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10379_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 02 03 05 14 18 19 20 21 22 23

otic capsule

moderate

regional

moderate expression: see section 01 02 03 15 16 17 18 19 20 21 22 23 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

meckel's cartilage

moderate

regional

moderate expression: see section 10 11 12 18 19 weak expression: see section 06 07 08 09 20 21 22

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 14 15 16

lower jaw molar

moderate

regional

moderate expression: see section 17

upper jaw incisor

moderate

regional

moderate expression: see section 13

liver lobe

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

axial skeleton

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

cranium

strong

regional

strong expression: see section 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 01 02 03 04

pectoral girdle and thoracic body wall skeleton

moderate

regional

moderate expression: see section 09 10 11 12 13 21 weak expression: see section 17 18 19 20 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1731
Entity Detected:	S100a9, S100 calcium binding protein A9 (calgranulin B) ( MGI:1338947)
Sequence:	sense strand is shown >T1731 TGGCCTCGAGCCAGAATTCGGCACGAGGCTTTAGCCTTGAAGAGCAAGAAGATGGCCAACAAAGCACCTT CTCAGATGGAGCGCAGCATAACCACCATCATCGACACCTTCCATCAATACTCTAGGAAGGAAGGACACCC TGACACCCTGAGCAAGAAGGAATTCAGACAAATGGTGGAAGCACAGTTGGCAACCTTTATGAAGAAAGAG AAGAGAAATGAAGCCCTCATAAATGACATCATGGAGGACCTGGACACAAACCAGGACAATCAGCTGAGCT TTGAGGAGTGTATGATGCTGATGGCAAAGTTGATCTTTGCCTGTCATGAGAAGCTGCATGAGAACAACCC ACGTGGGCATGGCCACAGTCATGGCAAAGGCTGTGGGAAGTAATTAAGAGGTCAGCCATGTGACAGCTGC CCAACCAAGTCTAAAGGGAATGGCTTACTCAATGGCCTTTGTTCTGGGAAATGATAAGATAAATAATAAA TAAGTCTTTATCCATTCAAAAAAAAAAAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:480712 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:480712 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10380 same embryo
	EMAGE:10382 same embryo
	EMAGE:10383 same embryo
	EMAGE:10381 same embryo
	EurExpress:euxassay_000521 same experiment
	MGI:4827854 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source