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EMAGE:10510

Rela v-rel reticuloendotheliosis viral oncogene homolog A (avian) ( MGI:103290)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510
"Pseudo-wholemount" of euxassay_000778. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000778_01 euxassay_000778_02 euxassay_000778_03 euxassay_000778_04
EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510
euxassay_000778_05 euxassay_000778_06 euxassay_000778_07 euxassay_000778_08 euxassay_000778_09
EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510
euxassay_000778_10 euxassay_000778_11 euxassay_000778_12 euxassay_000778_13 euxassay_000778_14
EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510
euxassay_000778_15 euxassay_000778_16 euxassay_000778_17 euxassay_000778_18 euxassay_000778_19
EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510 EMAGE:10510
euxassay_000778_20 euxassay_000778_21 euxassay_000778_22 euxassay_000778_23 euxassay_000778_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10510Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10510_wholemount_strong.wlz
10510_wholemount_moderate.wlz
10510_wholemount_weak.wlz
10510_wholemount_possible.wlz
10510_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10510_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diaphragm
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23 24
limb
strong strong
regionalstrong expression: see section 01 02 03 04 05 21 22 23 24
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 19 20 21 weak expression: see section 22
nasal capsule
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
tongue
moderate moderate
homogeneousmoderate expression: see section 10 11 12 13 14 15
hindgut
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 06 07 08 18 19 20
lower jaw molar
moderate moderate
regionalmoderate expression: see section 08 09 10 12 13 14 15 16 17
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 08 10 16 17 18 19 20
upper jaw molar
moderate moderate
regionalmoderate expression: see section 09 10 14 15 16
axial skeleton
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1019
Entity Detected:Rela, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ( MGI:103290)
Sequence:sense strand is shown

>T1019
TCTCGAGNCTGTTGGCCTACTGGATTTTTCCGCCTCTGGCGAATGGCTTTACTTTAGCGCGNCGTGGGCT
CAGCTGCGANCCCGGCCCCGCCCCCGGGACCCTGACCATGGACGATCTGTTTCCCCTCATCTTTCCCTCA
GAGCCTGCCCAGGCTTCTGGGCCTTATGTGGAGATCATCGAACAGCCGAAGCAACGGGGCATGCGATTCC
GCTATAAATGCGAGGGGCGCTCAGCGGGCAGTATTCCTGGCGAGAGAAGCACAGATACCACCAAGACACA
CCCCACCATCAAGATCAATGGCTACACAGGACCAGGAACAGTTCGAATCTCCCTGGTCACCAAGGATCCA
CCTCACCGGCCTCATCCACATGAACTTGTGGGGAAGGACTGCCGGGATGGCTACTATGAGGCTGACCTCT
GCCCAGACCGCANTATCCATAGCTTCCAGAANCTGGGGATCCAGTGTGTGAAGAAGCGAGACCTGGAGCA
ANCCATTAGCCNGCGANTCCAGACCAACAATAACCCCTTTCACGNTCCTATAGAGGAGCA
Notes:The probe template was PCR amplified from IMAGE:2076600 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2076600 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10509 same embryo
 EMAGE:10511 same embryo
 EMAGE:10508 same embryo
 EurExpress:euxassay_000778 same experiment
 MGI:4827690 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS