EMAGE mouse gene expression spatial database

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EMAGE:10514

Tmem54 transmembrane protein 54 ( MGI:1913510)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003286. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003286_01	euxassay_003286_02	euxassay_003286_03	euxassay_003286_04

euxassay_003286_05	euxassay_003286_06	euxassay_003286_07	euxassay_003286_08	euxassay_003286_09

euxassay_003286_10	euxassay_003286_11	euxassay_003286_12	euxassay_003286_13	euxassay_003286_14

euxassay_003286_15	euxassay_003286_16	euxassay_003286_17	euxassay_003286_18	euxassay_003286_19

euxassay_003286_20	euxassay_003286_21	euxassay_003286_22	euxassay_003286_23	euxassay_003286_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10514_wholemount_strong.wlz

10514_wholemount_moderate.wlz

10514_wholemount_weak.wlz

10514_wholemount_possible.wlz

10514_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10514_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2338
Entity Detected:	Tmem54, transmembrane protein 54 ( MGI:1913510)
Sequence:	sense strand is shown >T2338 CTCGAGGCCAGAATTCGTCGACATGGTGCCCTTGGACTCCTGGCCTCCATTGCAGTGACCTTCGCCACCA AGGGTAGAGCACTATTGGCGGCCTGTACTTTTGAGAACCCTGAACTCCCAACACTGGCACCTGACTGTCC TTTTGACCCTACTCGAATTTATAGCTCTAGCCTGTGCCTCTGGGCCATCTCTCTCATATTCTGTCTGGCG GAGAGTATGTCTGCCGTGCGCTGCGCCCAGCTCATGCATGGACTACTGGAACTAAGGCCCTGGTGGGGGA AGAGCTACCACCACACGATACAAGCAAGCCCAGAGCCCCTGGATGCTCATGACCTATTGAGCTGCGCCAG TTCTTGCAGCTGACCCTCTGACACTTCAGCAGCGCCATGGCTACTGAGGCACGTGACCACACCTGTATTG ACCCGTGCCCAACCAGCCATGGAAGTTTAGTGGGATCCAGGATTCCTAGAGCACGCCCATGAAAAGTCAA TGGACTACTGGGGACGTGGGAGAGGGCTTTGAGCGCCCTCCCCTGGCCACCGAGTACAATGCACTGAAAC AA
Notes:	The probe template was PCR amplified from IMAGE:1167629 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1167629 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10515 same embryo
	EMAGE:10513 same embryo
	EMAGE:10512 same embryo
	EurExpress:euxassay_003286 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source