EMAGE mouse gene expression spatial database

EMAGE:10517

Il13 interleukin 13 ( MGI:96541)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000820. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000820_01	euxassay_000820_02	euxassay_000820_03	euxassay_000820_04

euxassay_000820_05	euxassay_000820_06	euxassay_000820_07	euxassay_000820_08	euxassay_000820_09

euxassay_000820_10	euxassay_000820_11	euxassay_000820_12	euxassay_000820_13	euxassay_000820_14

euxassay_000820_15	euxassay_000820_16	euxassay_000820_17	euxassay_000820_18	euxassay_000820_19

euxassay_000820_20	euxassay_000820_21	euxassay_000820_22

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3878
Entity Detected:	Il13, interleukin 13 ( MGI:96541)
Sequence:	sense strand is shown >T3878 GGCACGAGTTTAAATGAGTCTGTCAGTATCCCGGTGGGACATGGTTTGCTGCCTATGCCCTGGGGGTCCA GCATTGAAGCAGTGGGCTCTGGGGTCCCTGGCAATATTACTGTATACATAATTCTGCTACCTCACTGCAG CCTCCAGGTCTCACCCCAGGCAGGAGATGGGAGGGGAGGCCAGAGCAACACTCCTGTCTGCCACGGAGCA ACCAGCCCTCAGCCATGAAATAACTTATTGTTTTGTTCTTATATTTAAAGTATTAAATAGCTTAGCAAAG AGTTAATAATATATGGAAGAATGGCCTGTTACACTCAAGGTGATGTGTAGTGAATGGGGGGAGGGTGGTG GGTTTGTCACTGAACAAAATTTTCATTGACTGTCAAACTAGAAACCGGAAATAAAGATGGTGACAGATAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1140097 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1140097 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10519 same embryo
	EMAGE:10521 same embryo
	EMAGE:10518 same embryo
	EMAGE:10520 same embryo
	EMAGE:10516 same embryo
	EurExpress:euxassay_000820 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source