EMAGE mouse gene expression spatial database

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EMAGE:10553

Igsf5 immunoglobulin superfamily, member 5 ( MGI:1919308)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000968. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000968_01	euxassay_000968_02	euxassay_000968_03	euxassay_000968_04

euxassay_000968_05	euxassay_000968_06	euxassay_000968_07	euxassay_000968_08	euxassay_000968_10

euxassay_000968_11	euxassay_000968_12	euxassay_000968_13	euxassay_000968_14	euxassay_000968_15

euxassay_000968_16	euxassay_000968_17	euxassay_000968_18	euxassay_000968_19	euxassay_000968_20

euxassay_000968_21	euxassay_000968_22	euxassay_000968_23	euxassay_000968_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10553_wholemount_strong.wlz

10553_wholemount_moderate.wlz

10553_wholemount_weak.wlz

10553_wholemount_possible.wlz

10553_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10553_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09 10 weak expression: see section 04

hindgut

moderate

regional

moderate expression: see section 12 13

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

kidney calyx

moderate

homogeneous

moderate expression: see section 17 19 weak expression: see section 16 20

kidney pelvis

moderate

homogeneous

moderate expression: see section 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T970
Entity Detected:	Igsf5, immunoglobulin superfamily, member 5 ( MGI:1919308)
Sequence:	sense strand is shown >T970 TCCTCGAGNCTGTTGGCCTACTGGAGTCAGAAGTGTTCCCGTGTTTCTATAGAGAGCAGCCAGAAGCCGG GAAGCCCTGTGTGAACGAGATACAGAGATTTACCTGCCTGAGGTAAGGAAGATCATGCTGAGATGGAGGG CAGCTGGAGAGATGTCCTGGCTGTGCTGGTCATCCTGGCTCAGCTGACAGCTTCCGGATCCAGTTATCAG ATCATAGAAGGTCCTCAGAATGTAACAGTCCTAAAGGACTCAGAGGCTCACTTCAACTGCACCGTGACTC ACGGCTGGAAGCTTCTCATGTGGACTCTTAACCAAATGGTGGTGCTGAGTCTCACCACCCAAGGACCCAT CATCACCAACAACCGCTTCACCTATGCCAGTTACAACAGCACTGACAGCTTCATCTCGGAGTTGATCATC CATGATGTGCAGCCCAGTGACTCGGGATCCGTGCAATGCAGCCTGCAGAACAGCCATGGGTTTGGATCTG CCTTCCTCTCAGTGCAAGTCATGGGGACCCTGAACATTCCTAGCAACAACCTTATAGTCACTGAGG
Notes:	The probe template was PCR amplified from IMAGE:1971395 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1971395 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10552 same embryo
	EMAGE:10554 same embryo
	EMAGE:10556 same embryo
	EMAGE:10555 same embryo
	EurExpress:euxassay_000968 same experiment
	MGI:4825547 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source