EMAGE mouse gene expression spatial database

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EMAGE:10568

Psmb4 proteasome (prosome, macropain) subunit, beta type 4 ( MGI:1098257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000823. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000823_01	euxassay_000823_02	euxassay_000823_03	euxassay_000823_04

euxassay_000823_05	euxassay_000823_06	euxassay_000823_07	euxassay_000823_08	euxassay_000823_09

euxassay_000823_10	euxassay_000823_11	euxassay_000823_12	euxassay_000823_13	euxassay_000823_14

euxassay_000823_15	euxassay_000823_16	euxassay_000823_17	euxassay_000823_18	euxassay_000823_19

euxassay_000823_20	euxassay_000823_21	euxassay_000823_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10568_wholemount_strong.wlz

10568_wholemount_moderate.wlz

10568_wholemount_weak.wlz

10568_wholemount_possible.wlz

10568_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10568_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3921
Entity Detected:	Psmb4, proteasome (prosome, macropain) subunit, beta type 4 ( MGI:1098257)
Sequence:	sense strand is shown >T3921 CTAAGATGGAAGCGTTTTGGGAGTCACGGGCTGGCCACTGGGCCGGGGGTCCGGCTCCGGGGCAGTTTTA CCGCATCCCGTCCACCCCCAGCGGCCTCATGGACCCGGCGTCGGCGCCCTGCGAGGGTCCCATCACTCGG ACCCAGAACCCCATGGTGACCGGGACATCGGTACTCGGGGTGAAGTTCGACGGCGGAGTGGTGATTGCTG CAGACATGCTGGGCTCCTACGGCTCCCTGGCTCGTTTCCGCAATATCTCTCGTATTATGCGAGTCAACGA CAGCACTATGCTGGGTGCCTCGGGAGACTACGCTGATTTCCAGTATTTGAAACAAGTTCTCGGCCAGATG GTGATTGATGAAGAGCTGTTGGGAGATGGACACAGCTATAGCCCTAGAGCTATTCATTCATGGTTGACAA GAGCCATGTACAGCCGCCGCTCCAAGATGAATCCCCTGTGGAACACCATGGTCATTGGAGGCTATGCTGA CGGAGAAAGCTTCCTCGGTTATGTGGACATGCTTGGTGTAGCTTATGAAGCCCCTTCACTGGCCACTGGT TATGGTGCATACTTGGCTCAGCCTCTGCTTCGAGAAGTTCTAGAGAAGCAGCCAGTGCTGAGTCAGACTG AGGCTCGGGAGCTTGTGGAGCGCTGCATGAGAGTGCTGTACTACAGAGATGCCCGTTCGTATAACCGGTT TCAAATTGCCACTGTGACTGAAAAGGGTGTCGAGATAGAAGGACCGCTGTCCGCACAGACCAACTGGGAC ATCGCTCACATGATCAGTGGCTTTGAATGAAATCCAGTTGAATGTCCAGAGTGTTGAAGCTTTGCTCTTC TAAACGTGACTGTAGCTGGCTCAAAGGCAGACTTTTGTAAAATAAATCAGTCGTTTGAAGTGTTCAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2654421 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2654421 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10571 same embryo
	EMAGE:10570 same embryo
	EMAGE:10569 same embryo
	EurExpress:euxassay_000823 same experiment
	MGI:4827477 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source