EMAGE mouse gene expression spatial database

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EMAGE:10600

Hook2 hook homolog 2 (Drosophila) ( MGI:2181664)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000942. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000942_01	euxassay_000942_02	euxassay_000942_03	euxassay_000942_04

euxassay_000942_05	euxassay_000942_06	euxassay_000942_07	euxassay_000942_08	euxassay_000942_09

euxassay_000942_10	euxassay_000942_11	euxassay_000942_12	euxassay_000942_13	euxassay_000942_14

euxassay_000942_15	euxassay_000942_16	euxassay_000942_17	euxassay_000942_18	euxassay_000942_19

euxassay_000942_20	euxassay_000942_21	euxassay_000942_22	euxassay_000942_23	euxassay_000942_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10600_wholemount_strong.wlz

10600_wholemount_moderate.wlz

10600_wholemount_weak.wlz

10600_wholemount_possible.wlz

10600_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10600_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 18 19 20 21 weak expression: see section 07 08 17

foregut-midgut junction

moderate

homogeneous

moderate expression: see section 14 15 16 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 18 19 20 weak expression: see section 10 11 12 13 14 21 22

esophagus

moderate

regional

moderate expression: see section 13 weak expression: see section 12

stomach

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 weak expression: see section 03

hindgut

moderate

homogeneous

moderate expression: see section 16 17

midgut

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

kidney calyx

weak

homogeneous

weak expression: see section 10 11 19 21 22

kidney pelvis

weak

homogeneous

weak expression: see section 20

laryngeal cartilage

moderate

regional

moderate expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T433
Entity Detected:	Hook2, hook homolog 2 (Drosophila) ( MGI:2181664)
Sequence:	sense strand is shown >T433 GTTTCACCCCCCTGTGCCAGTCCCCAGGACCTGAGCAGCGGACTCGCCATAGCCCATGTGCTGAATCAAA TAGACCCTTCCTGGTTCAACGACGAATGGCTCCAGGGCATCTCAGAGGACTCAAGCCCCAGCTGGAGGTT GAAGGTCAGGAAACTGGAGAAGATCTTACAGAGCTTGGTGGAATACTCGAAGAATGTTCTGGGGCACCCT GTGTCAGATCAGCATCTCCCAGATGTGAGCCTCATTGGCGAGTTCTCAAATCCTGCAACTCGGCAAACTG CTTCAGTTGGTACTGGGCTGTGCTATCAGTTGTGAGAAAAAACAGGAGTATATCCAAAGAATTATGACCC TGGAGGAGTCTGTTCAACATGTAGTGATGGAAGCCATCCAGGAGCTCATGACCAAAGACGCTCCTGATTC CCTGTCACCAGAGAATTACGGGAATTTTGATACACAGTCCCGCAGGTACTATTTCCTGAGTGAGGAGGTG GAGGAG
Notes:	The probe template was PCR amplified from IMAGE:3164670 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3164670 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10601 same embryo
	EMAGE:10599 same embryo
	EurExpress:euxassay_000942 same experiment
	MGI:4825408 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source