EMAGE mouse gene expression spatial database

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EMAGE:10647

Tmem68 transmembrane protein 68 ( MGI:1919348)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003250. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003250_01	euxassay_003250_02	euxassay_003250_03	euxassay_003250_04

euxassay_003250_05	euxassay_003250_06	euxassay_003250_07	euxassay_003250_08	euxassay_003250_09

euxassay_003250_10	euxassay_003250_11	euxassay_003250_12	euxassay_003250_13	euxassay_003250_14

euxassay_003250_15	euxassay_003250_16	euxassay_003250_17	euxassay_003250_18	euxassay_003250_19

euxassay_003250_20	euxassay_003250_21	euxassay_003250_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10647_wholemount_strong.wlz

10647_wholemount_moderate.wlz

10647_wholemount_weak.wlz

10647_wholemount_possible.wlz

10647_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10647_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4302
Entity Detected:	Tmem68, transmembrane protein 68 ( MGI:1919348)
Sequence:	sense strand is shown >T4302 GGCACTACCCGCGTGTCTGCTTCTCCCGGCTCTGCCGCCGACCAGCTGCCGAGGTCGCCGATTTCCTGTG ACTTTCTTGTCAGAGTATATGCATTGAAGCTAAAGACAAGATATTTGTGTAATTTGTGTAATCTGGCATT GAAGTTGGAACATATGGCAATGAAGAAAGAGACAGGATTTGGGATTTTCATCTAAGAACATGATAGATAA CAACCAAACCTGTGCTGCAGGACAGGACTCTGTGCCCTATGTGACCTGTATGATTTATGTGCTTGAGGAG TGGTTGGGTGTGGAGCAATTGGAGGACTATCTGAATTTTGCAAACCATCTTTTGTGGGTTTTCACCCCAC TGATACTTTTAATACTTCCTTATTTTACCATCTTTCTTCTCTACCTTACTATTATTTTCCTCCATATCTA CAAGAGGAAGAATGTATTAAAAGAAGCCTACTCTCATAACTTGTGGGATGGTGCAAGGAAAACGGTGGCA ACTCTGTGGGATGGACATGCGGCTGTTTGGCATGGTTATGAAGTTCATGGGATGGAAAAGATACCAGAAG GAGCTGCACTTATAATTTTTTATCATGGAGCCATCCCCATAGACTTTTACTACTTCATGGCTAAAATTTT CATCCAGAAAGGCAGAACTTGCCGAGTAGTAGCTGACCACTTTGTCTTTAAAATTCCAGGGTTCAGTTTA TTACTTGATGTGTTTTGTGCTCTTCATGGACCAAGAGAAAAATGTGTTGAAATCTTGAGGAGTGGTCACT TGTTAGCTATCTCACCGGGCGGAGTTCGAGAAGCCTTACTTAGTGATGAAACCTACAACATCATATGGGG TAATCGTAAAGGCTTTGCTCAGGTTGCAATTGATGCAAAAGTGCCCATTATTCCTATGTTTACACAAAAT ATTCGAGAAGGATTTAGATCACTTGGAGGCACAAGATTATTTAAGTGGCTTTATGAAAAATTCCGCTATC CATTTGCTCCAATGTATGGAGGTTTTCCTGTAAAGTTGCGGACCTTCTTAGGTGATCCCATACCATATGA CCCAAAGGTAACAGCAGAAGAATTAGCCGAAAAGACCAAGAATGCTGTTCAAGCTTTGATTGACAAGCAC CAAAGGATACCGGGGAACATTAGGAGTGCTCTGCTGGACCGCTTTCATAAAGAGCAGAAGGCTCATTAGC AGATAGCTTAATGTGTCTGCAGTGACAAGTTTGCATCTGTGGTACTGTCTTGTAGGTTGTATATTGATTA GTGTTTTTAAAACGTTGTGCTAATGAACATCTTTCTTTAGAACTTAAACTTTATATGGTTAATCTTATCC TTTCAATCAGTTGTGTCAAATTTAATAAGTAGCTCATCATTTGCTTGACTCAGCAAATGATCATGTTGCA TTTGCAGTTTTTTTAGTAGTATATGATAAGCACAGAATCTTAGAGTTAAGTCTTCCTTGGTATCCGCAGA TTAGGTCAGGCAGACTTTACTAAATCATGTTTCTTATGTTTGTGTCTGTTGTGCCAATATGATAGTAGGG TGTAGCACTTGCTTAATTAAGATGAGTGATGCCTTTATGTATCTGTCCTTCATTCCATTCACAACTGCTG GCAAGTTGTGGAACTCTTTTGCACCTCAATGATTTATGTTAAGCATGCTAATACTGTGCTGATGAACATT ATTTTAAGTTGCTTATATCTACTGTGATATATCCATATATGATAGATATCTGTAGTGAGAATCTATAATT GAATTGATTCCTAAATCCTCTAGGAGTTATGTATATAATTTTGTCCATTGGATGTTGAAACTACTCCTTC AGGTATGATGTCCAGTTGCCAAGCACATTGAAAGAGATGTACTATTTTATAAATCAAATCATTGTTATTT GTAGTATTTATTAACCGAATTGTTTAGTATTTATTTTAAATAAGTATCTAAGGAAATGTATAGGATGAGT TGTCAGCACAGTGTTTTATGGTGTAAAGACAAAATCATGGGCATGCTCTGCAGTTCATGTTAGAAGTGGG TTCTAGGCTAAGGAATAACATGCTAAGACAGCTCTGGTCTCGCTCAGTATCCGGGCTCTCTTCCTGTGCT TTGCAGCCAGTAACGTGGTTATTATTTTAATAAAACGTACTTGAATGATAAACTTCTGTAAACATGGAGC TCTCTGAAAATGTAATTGCTTTTGATTTGTTGTCTTTTTGAAAACTTATTTTAAAAGTATTTAAACAACA AAGTATCATAAGCTCACTGCAGTATGTCTGTCTCCTACCACCTTTGTATACTTGGGTTTTATATGGACTT TTTAACTACAAGTGTTTATTTATGACATATATAATGTATATAGAAATGATGTGAAAATAAAGCTGAATTG CACCTCCTTCTCTAGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506688 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506688 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10644 same embryo
	EMAGE:10645 same embryo
	EMAGE:10643 same embryo
	EMAGE:10646 same embryo
	EurExpress:euxassay_003250 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source