EMAGE mouse gene expression spatial database

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EMAGE:10679

Snrpa1 small nuclear ribonucleoprotein polypeptide A' ( MGI:1916231)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000860. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000860_01	euxassay_000860_02	euxassay_000860_03	euxassay_000860_04

euxassay_000860_05	euxassay_000860_06	euxassay_000860_07	euxassay_000860_08	euxassay_000860_09

euxassay_000860_10	euxassay_000860_11	euxassay_000860_12	euxassay_000860_13	euxassay_000860_14

euxassay_000860_15	euxassay_000860_16	euxassay_000860_17	euxassay_000860_18	euxassay_000860_19

euxassay_000860_20	euxassay_000860_21	euxassay_000860_22	euxassay_000860_23	euxassay_000860_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10679_wholemount_strong.wlz

10679_wholemount_moderate.wlz

10679_wholemount_weak.wlz

10679_wholemount_possible.wlz

10679_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10679_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 09 10 11 17 18 19 weak expression: see section 08 20

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15 16

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 17 18 19 20

dorsal root ganglion

strong

regional

strong expression: see section 17 18 19 moderate expression: see section 10 11 12

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

liver lobe

strong

homogeneous

strong expression: see section 01 02 03 04 05 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

kidney calyx

weak

homogeneous

weak expression: see section 08 09 10 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T904
Entity Detected:	Snrpa1, small nuclear ribonucleoprotein polypeptide A' ( MGI:1916231)
Sequence:	sense strand is shown >T904 CCTCNAGNCTGTTGGCCTACTGGACTGTGGGACACGGCACTGGCGGGAGGCGCAGGCTGGTGTCGGAGGT GAGGGTCCGCGGGCTGCGACAGGATGGTGAAGCTGACGGCGGAGCTGATCGAGCAGGCGGCGCAGTACAC AAATGCCGTACGGGACCGTGAACTGGACCTCCGGGGATATAAAATTCCGGTTATTGAGAATCTGGGTGCC ACCTTAGACCAGTTTGATGCTATTGATTTTTCTGACAATGAGATCCGGAAACTGGACGGTTTCCCTTTGT TGAGAAGACTGAAAACATTATTAGTGAACAACAACAGAATTTGCCGTATAGGTGAGGGACTTGACCAGGC TCTTCCCTGTCTGACAGAGCTCATCCTCACCAACAACAGTCTAGTGGAACTGGGTGATCTGGACCCGTTG GCATCTCTCAAATCACTGACATATCTAAGCATCCTAAGAAACCCTGTAACCAATAAGAAGCATTATCGAC TTTATGTGATTTATAAAGTTCCACAAGTCAGAGTACTGGACTTTCAGAAAGTGAAACTCAAA
Notes:	The probe template was PCR amplified from IMAGE:1924594 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1924594 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10676 same embryo
	EMAGE:10677 same embryo
	EMAGE:10680 same embryo
	EMAGE:10675 same embryo
	EMAGE:10678 same embryo
	EurExpress:euxassay_000860 same experiment
	MGI:4828354 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source