EMAGE mouse gene expression spatial database

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EMAGE:10721

Fbxw4 F-box and WD-40 domain protein 4 ( MGI:1354698)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003165. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003165_01	euxassay_003165_02	euxassay_003165_03	euxassay_003165_04

euxassay_003165_05	euxassay_003165_06	euxassay_003165_07	euxassay_003165_08	euxassay_003165_09

euxassay_003165_10	euxassay_003165_11	euxassay_003165_12	euxassay_003165_13	euxassay_003165_14

euxassay_003165_15	euxassay_003165_16	euxassay_003165_17	euxassay_003165_18	euxassay_003165_19

euxassay_003165_20	euxassay_003165_21	euxassay_003165_22	euxassay_003165_23	euxassay_003165_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10721_wholemount_strong.wlz

10721_wholemount_moderate.wlz

10721_wholemount_weak.wlz

10721_wholemount_possible.wlz

10721_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10721_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon roof plate

weak

regional

weak expression: see section 12 13 15 16 17

midbrain ventricular layer

weak

regional

weak expression: see section 10 11 12 13 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5377
Entity Detected:	Fbxw4, F-box and WD-40 domain protein 4 ( MGI:1354698)
Sequence:	sense strand is shown >T5377 GCGGCATCCCGGGAACCCGGACGGCCATGGCGGAGGACGCGGCGGAGGATGCCGCAGCGGCGGCCGTGGA GCCGGCCACCCGTCCGGCCGCGGGGCCCGCGCTCTGGCGCCTGCCGGAGGAGCTGCTGCTGCTCATCTGC TCCTACCTCGACACGCGGGCCCTCGGCCGCCTGGCCCAAGTCTGCCGCTGGCTGCGGCGCTTCACCAGCT GCGACCTGCTCTGGCGCCCGATAGCCCGGGCCTCGCTCAACACCGGCTTCACGCGGCTCGGCACCGACCT CATGGCAGGCATTCCAGTAAAGGAGCGCGTGAAACTGTCTCAGAACTGGCGGCTGGGGCGCTGCCGAGAT CGGATTCTGCTGAAGTGGAGATATAGTCAGATGCCTTGGATGCAGCTACAGGATGCTTCTCTGTACCTAT CACAGGCTAATTTCATCCTGGCCTACCAGTTCCGCCCGGATGGTGCAAGCTTGAACCGACGGCCCTTCAG AGTCTTCTCTGGGCATGATGAGGACGTCTGTCACTTTGTGCTGGCCAACTCCCACATCGTCAGTGCAGGA GGAGACGGGAAGATCGGTGTTCACAAGATCCACAGCACCTTCACTGTCAAGTACTCGGCTCACGAGCAGG AGGTGAACTGTGTGGACTGTAAAGGTGGCATCATTGTGAGTGGCTCCCGGGACAGGACAGCCAAGGTATG GCCTCTGGCCTCGGGCCGGCTGGGGCAGTGCTTACACACCATCCAGACCGAAGACCGAGTCTGGTCCATT GCTATCAGCCCATTACTCAGCTCTTTTGTGACTGGAACAGCTTGTTGTGGGCACTTCTCACCTCTGAGAA TCTGGGACCTCAACAGTGGGCAGCTGATCACACATCTGGGCAGTGACTTTCCCCCAGGGGCTGGGGTGCT AGATGTCATGTATGAGTCCCCCTCTACACTGCTGTCCTGTGGTTATGACACCTATGTTCGCTATTGGGAC CTCCGCACAAGTACACGGAAATGTGTCATGGAGTGGGAGGAGCCTCATGACAGTACCTTCTACTGCCTAC AGACAGACGGCAACCACCTGCTGGCCACAGGCTCCTCCTACTACGGTCTCGTGCGGCTGTGGGACCGACG CCAGCGGGCTTGCCTGCATGCCTTCTCATTGACGTCGACCCCACTCAGCAGCCCTGTGTACTGCCTGCGG TTCACCACTAGGCATCTCTATGCTGCGCTGTCTTACAACCTCCACGTCTTAGACTTTCAGAACCCATGAC AGAGCAGCCCTGCTTAGGGGGCTGGGCAAGTCAGCTACTCAGGGACCTCTCCTGCCTGGCAGGTGAGGGG ATACCTCACTCTTCTTCCCAGCCCTGCTGAGTGAGCTCCTAGGTCTCTGGCCACCATGCCCTGGCACCTG AAGCACTGAGTCCTGGACGCTGGGCTGACTCTGACGTGGGCCCTCCTGTGCTCTTAGGAGAATGTACCTG AACTTAGGTGTTTACAGTAGGGTCAGGCCTTCTCCTGAGCCCTTCCCAGCACTACTGCAGCCTACAGAGT GAAATGAGGGCTCTCACCATCCCGGACAGAAAGAGATGCAGTTGCTAGAACTTGTGCTGGTCCCAGCCAC CTCCTCAGTCAGATGGTAACTCTGGCCAACATCTTGGAGCTGGGCTCGCTCCTGAGGTACCAGTCTGCTT TGCTTTGAAACGAAGGAAGGACAAAGAAAGCCTGTCAGCTTGGGGTTCCGGACTAGCTGTTCCTCAGTCC TGCTATGCGAAACATGCTACCATTGTAATTTTTATAAAAATAAAATTTAATTGTTGAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364017 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364017 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10725 same embryo
	EMAGE:10724 same embryo
	EMAGE:10723 same embryo
	EMAGE:10722 same embryo
	EurExpress:euxassay_003165 same experiment
	MGI:4824816 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source