EMAGE mouse gene expression spatial database

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EMAGE:10740

Ppp1r17 protein phosphatase 1, regulatory subunit 17 ( MGI:1333876)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001162. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001162_01	euxassay_001162_02	euxassay_001162_03	euxassay_001162_04

euxassay_001162_05	euxassay_001162_06	euxassay_001162_07	euxassay_001162_08	euxassay_001162_09

euxassay_001162_10	euxassay_001162_11	euxassay_001162_12	euxassay_001162_13	euxassay_001162_14

euxassay_001162_15	euxassay_001162_16	euxassay_001162_17	euxassay_001162_18	euxassay_001162_19

euxassay_001162_20	euxassay_001162_21	euxassay_001162_22	euxassay_001162_23	euxassay_001162_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10740_wholemount_strong.wlz

10740_wholemount_moderate.wlz

10740_wholemount_weak.wlz

10740_wholemount_possible.wlz

10740_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10740_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 19

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 16

cervical ganglion

weak

regional

weak expression: see section 09 17

thoracic ganglion

weak

regional

weak expression: see section 12 14

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 18 19

neural retina

weak

regional

weak expression: see section 01 02 03 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4329
Entity Detected:	Ppp1r17, protein phosphatase 1, regulatory subunit 17 ( MGI:1333876)
Sequence:	sense strand is shown >T4329 GAGCCCCAGCGCGGTGAGAAGAACTGCCTTTACCTTCTCGGCTCAACGCCTGCACTGATTGAAAAGACTC TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTCAACTGAGGATGTCCACTGAGAT GATGACCACCGAGCCAGTGCCGCCTCTGGAGCTTTCAGACGACATACTAGGCAAGCTGGATCCTCAGTGC AGCCCCTCAGATGACCTTTCAGACCAGTTCATTAAGGACTGTGACCTCAAAAAGAAGCCAAGGAAGGGGA AGAATGTACAGGCCACTCTGAATGTTGAGTCAGATCAAAAGAAACCCAGGAGGAAAGACACACCAGCAGT GCACATCCCACCTTTCATCCCAGGTGTGATTTCGGAACACTTAATCAAAAGATATGATGTTCAAGAGAGA ATTCCAAAGGCCAAATCAGGCCCAGCTCTTCATAACTCTGATATGGAACAAAAAAGGCCAAGGAGAAAAG ACACGCCTGCCTTGCACATGCCTCCCTTTGTTGCAGGTCTGACTCTGCTCCGGGATGAGAGTGCAGGAGT GATTCTAGAGGATGAAGAAATGGATGGTGACAAGCTAGCTATTTAAAGACATCTCCCCTGAGATTGCGTG TAAATAGATTACACTGGTTCCCTATGGTGATCTAGAGGGAAAAAATAGATTTGTCTCTGCTCTCATTTTG TCCTAGTCGACTCTAAGATCTAGACGCCTCCTCCCCAGGAGACCTACTACTCAGATTGCTGGTCAGGTCA CCTCTTTGTCTTTCCTTTCTTGCTTTTTAACCCCACCTCCTAAACTTTCATTTTTCTATACTGTGCAAAG GAAAGTGATTTTTTTCTTCCAAAGTAAGAGAGGTGAGACCTCAGTGCTTCATGTCTGCAAGCAAAAATCC TGGATAAAAGATGGGGAGGGGGGAGGGGAGGCGGTCCTTTCCACCTGGTTTAGTAGCAGGGCAGTCCGAT TCAGCATCAAACACTCAGGAGTACAGCAAGGCCACAGCTGCTGGTTAGACTGTAAGAACATTTGGACTCT AACTCATGGACTGCTATGATGAAATGTTTTTTTCTTTCTTCCTTCCTATCTTGAGAGTCCAACTCTGATC ATCCTCTCCTCTGAAGACCCTCATTACAGATGTTATAATGGTTCAAGAAGAGGAAGCCAGCCCATTATGC AAGAATAAAAGACATCAAAGGCAGAGGATGCTGGTCTATGTAGGGAGGTCTTATACTTAATCTATTGTTA AAAAAAAAGAGAGTTCTTAACTATATTGGTTGTCGTGATATTTGTGAATTATGTCATAATGGGAGGTAAA TAAATACCAATGTAAGGAATTTATTTTTTTAAATCTAAGGCCAGTTGAAGGTTGTTCTGATATTTTTGCC ATCTCACTTATTTAGACTCTGATACGTTTTCTTATATAAGAGGTGGTGTTGTCTAGTGAACACAGATGTG CATTTGGCTCCTGTGCAGCTGGCAATCTCCAAGTGCCTGTGGGGACTCTTTGCATCTGTACTTCATCAGC CAAACAGCAGACAGAGAGGAGGTGAGGACTAAAAGGGTGAAAAGCAGAGTATCTAGTGTTCAGACCAAGG CAGGAGGCATGGGACAGCTCTGTACATACTGAGGTCCAGCTGTTTGTTATCCTTTCTGTCTTTCTGTTCC CCTTACAAAGATGTGAAAGATGCAATACATTTCAGAAATAAAGATACTTCCGCCCCCAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4511839 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511839 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10739 same embryo
	EMAGE:10743 same embryo
	EMAGE:10742 same embryo
	EMAGE:10741 same embryo
	EMAGE:10744 same embryo
	EurExpress:euxassay_001162 same experiment
	MGI:4825250 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source