EMAGE mouse gene expression spatial database

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EMAGE:10785

Pqlc2 PQ loop repeat containing 2 ( MGI:2384837)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003028. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003028_01	euxassay_003028_02	euxassay_003028_03	euxassay_003028_04

euxassay_003028_05	euxassay_003028_06	euxassay_003028_07	euxassay_003028_08	euxassay_003028_09

euxassay_003028_10	euxassay_003028_11	euxassay_003028_12	euxassay_003028_13	euxassay_003028_14

euxassay_003028_15	euxassay_003028_16	euxassay_003028_17	euxassay_003028_18	euxassay_003028_19

euxassay_003028_20	euxassay_003028_21	euxassay_003028_22	euxassay_003028_23	euxassay_003028_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10785_wholemount_strong.wlz

10785_wholemount_moderate.wlz

10785_wholemount_weak.wlz

10785_wholemount_possible.wlz

10785_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10785_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4832
Entity Detected:	Pqlc2, PQ loop repeat containing 2 ( MGI:2384837)
Sequence:	sense strand is shown >T4832 CCACGCGTCCGCGGATCCTGAAGCTGACACCGGCTGTGGATCTTGAACTGGTTGTCATGCACGCAGAGTG ACCACATAGGAACTCTGTGCAGGTGGCAAGGAACTGAGAGGGCGTCTTTCTTCCTGCTCCTTCAACCAGC CATACTCTGGCTTGGGCTGTGGCTTCCTTGTAGGATAAACCTTTGCCAGCCTCTGACCCCTTGCCTGTGT TCATACACCTGTACCTTCTGGGACCCTCCCTCTGCAGCCATGGTCTGGAGGACACTGGGCGCCAGCAACT TTTCCACCTGTCCCAATGGCTCTGTCCAGTGGATCTGGGATGTGTTTGGTGAATGTGCCCAGGATGGCTG GGATGAGGCCAGCGTGGGCCTGGGCTTGGTCTCCATTCTCTGCTTCGCTGCGTCTACCTTCCCCCAGTAC ATCAAAGCCTGCAAGACAGGCAACATGGACCAGGCCCTATCACTGTGGTTCCTGCTGGGCTGGATCGGTG GAGATTCCTGCAACCTCATTGGCTCCTTTCTGGCAGACCAGCTACCCCTGCAGACCTACACGGCTGTGTA TTATGTCCTGGCCGACCTGATGATGCTGACGCTGTACTTCCATTATAAGTTCAAGAAGCGACCTTCTCCG TTGTCTGCCCCTATCAATTCTGTGCTCTTGTTCATCTTGGGGACGGTGTGTATCACACCACTGTTGAGCA GCACTGATCCTGTGGCTGTCCCCAGGGAAGGCTTCCGGGGGCGGACACTCCTGTCTGTGGAGCCAGGCAA TAAGCCCTTCACAAAGAAGGAGGTCATCGGCTTTGTCATCGGCTCCGCCTCCAGCTTGCTGTACCTGCTC TCGAGGCTGCCTCAGATTCGCACCAATTTCATACGGCAGTCAACCCAAGGCATCTCCTACTCGCTGTTCG CACTGGTGATGCTGGGGAACACGCTGTACGGGCTCAGCGTGCTGCTCAAGAACCCCGAGGTGGGCCAGAG TGAGGGCAGCTACCTGCTGCACCACCTGCCCTGGCTCGTGGGCAGCCTGGGGGTGCTGCTGCTCGACACC ATCGTATCCTTCAAAGCGTGTGATGTTGGCACCGTTGTGTGGGCAAGACGGCCTCTGCTTCAGTGTGCAC ATCCCTGCCAGCCCACCCCTCTGTCTACATACTCATCCACCTGCCCATTAGCACATTCACCTATTCGTCC GTGTGTCCTTCCATCTACAATCCAGCCTTTAACCACCCTTCATTTACCCATCCAACCTCATCCACCCACC CATCACCCACTTAGACACCTATCCATCTATGCACTCACCTAATCATACACACATCCCATTTGTCCACCCA TCCTTCATCTATAATCCAACATTTCATCCAACCTCCATTTACCTGTCCATCCCCATCCATCTACCCACCG TCTGTCCATCATCCATCCATCCATCATCTGTCCATCCATCCATTATCTATCCATCCACCTATCTACCCAT CTTCCAGTGTATCTGCCCATCCAGCCTCCCACCCACCTGTCCATTCACTCGACTACCCATTCAGTTTCCC ATTTATCCATCCATTTATTCACCCACACCCACCCATCCACCCATCTGTGAGTCCATCAGTCCATCCATGA TGGGTGGACCCAGCCAGGACCAGTCCCATCAGTGAGCAAGGACAGAGCGTGCACTAGATTTTGGGGTGGT TGCCTAACTTCAGTGGCACCCAGCGCCGTGGAGTGAATCACCACTGGACTCTCCTGGTTTGGGGGAAACA GAGGCCCCAGCAGGTCATCGCACCGGGGCCGGGGGAGAAGTTATTCAGGCACAAGGGAACAGAGTCCCAG CACAGCCTGTGCAAAGGCCCCAAGGGACAGAAGAGTGTGTGCACTGTGGAAACGAGAGCTGAGTCATGGA GACAAGTTTGCGTAGGCTTCGCTCCTCTTCTCCGTTTCCTCCCTGATGACAAGGGTGAACTGCCAGTCAG ACCCTCAGGGTTTCAGTCACTGTCCCTGCTGGCATCAGTGTCTGAACACCCTCTCCATGTGACCTGTGAC CTGTTAGTGTTGCCAAGTGGTTCGGCTGGACGTTATTTGGCAAGAAGTAAACACGATCTTTGTGTCAACA TTTGGAGAGTCCCAGTGGGACGTGGTGAGAGAGAAAGGCTTAAAGTCTAGAGGGCCCACGTGGCCCAGCC TCTGGGTGTTGTAGGGAGGAAGAGCGCCCTATTCCAGGGTCCCTGATGGCAGAGTCAGGGCATCAAGGAG GGCACTCGGTGGTGGGAGAGCAAGGCGACAGGAGCAGGGCTGGCCCAGTGTGGCTGCAGTACCTTAACAG TGTCCTCAGATCTCCATCCAGTTCCTGGTGTACAGAAGCCATGAGACTGCTGCTGCCTCAGAGCGAGAGC CCCTCCTCCCCAGCTGATCATCGTCCTGGATGATGGGAACCTCAGCTGCCAAACCAGGAAGGATGAGGTG TGGGCAGAGATACCGCCGCCGCTGGACTGGGCCTAGGAGGCTGCCTGGAACTCTGGGCCTGGCTGCCTAG AACTCCTCCATGCCTGACCTTCAGACAGATCTGGAGTTGGCTTGTGCTGCACAGGGGCATACCTCTCAGG TGTATGACAGCTGCCCGAGGAGCCTCTAGGGCACCCATAGCTGAGACCCCAGAAGACGGTATGGTCACCT CCCATCTACCTACCTCAATCTGACTGTACTCTTGGTTACATCATAGTCAGGGGACCTAGGGTTGTCCCTC TGACTGGATACAACCTAGTAAATGGTACCTTCCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065163 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065163 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10786 same embryo
	EMAGE:10787 same embryo
	EMAGE:10783 same embryo
	EMAGE:10788 same embryo
	EMAGE:10784 same embryo
	EurExpress:euxassay_003028 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source