EMAGE mouse gene expression spatial database

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EMAGE:10802

Sart1 squamous cell carcinoma antigen recognized by T-cells 1 ( MGI:1309453)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003249. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003249_01	euxassay_003249_02	euxassay_003249_03	euxassay_003249_04

euxassay_003249_05	euxassay_003249_06	euxassay_003249_07	euxassay_003249_08	euxassay_003249_09

euxassay_003249_10	euxassay_003249_11	euxassay_003249_12	euxassay_003249_13	euxassay_003249_14

euxassay_003249_15	euxassay_003249_16	euxassay_003249_17	euxassay_003249_18	euxassay_003249_19

euxassay_003249_20	euxassay_003249_21	euxassay_003249_22	euxassay_003249_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10802_wholemount_strong.wlz

10802_wholemount_moderate.wlz

10802_wholemount_weak.wlz

10802_wholemount_possible.wlz

10802_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10802_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18

vibrissa

moderate

regional

moderate expression: see section 05 06 07 20 21 22

dorsal root ganglion

moderate

regional

moderate expression: see section 16 17 weak expression: see section 09 10 11

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 weak expression: see section 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 12 weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4873
Entity Detected:	Sart1, squamous cell carcinoma antigen recognized by T-cells 1 ( MGI:1309453)
Sequence:	sense strand is shown >T4873 CTAGGGTAGCGGCCCGGCTCCAGGCGGACGCAATAATCATGGGGTCGTCAAAGAAGCACCGTGGCGAGAA GGAGGCGGCCGGGACGACGGCTGCGGCCGGGACTGGGGGCACGACGGAGCAGCCGCCACGGCATCGCGAG CATAAGAAACACAAGCACCGGAGCAGCGGCGGCGGCAGCAGCGGCGGCGAACGACGGAAGCGGAGCCGGG AGCGTGGGAGTGAGCGCGGGAGCGGGCGGCGTGGGACCGAAGCCGAGGCCCGCAGCGGCGCGCACGGTCG CGAACGAAGCCAAGCGGAGCCCTCGGAGCGGCGCGTGAAGCGGGAGAAACGCGATGACGGCTACGAGGCC GCTGCCAGCTCCAAAGCCAGCTCTGGCGATGCCTCATCCCTCAGCATTGAGGAGACCAATAAACTCCGAG CAAAGTTGGGGCTGAAGCCCTTGGAGGTCAATGCTGTTAAGAAAGAGGCGGGCACCAAGGAGGAGCCCGT GGCAGCCGATGTTATCAACCCCATGGCCTTGCGACAGCGGGAAGAACTGCGGGAGAAGCTGGCAGCTGCC AAGGAAAAACGCTTGCTGAACCAAAAATTGGGGAAAATAAAGACACTGGGGGAGGATGACCCCTGGCTGG ATGATACTGCAGCCTGGATAGAGAGGAGCCGGCAGCTACAGAAAGAGAAGGACCTGGCAGAGAAGCGGGC TAAGCTACTAGAGGAGATGGACCAAGAGTTTGGTGTGAGCACTTTAGTGGAGGAGGAGTTTGAGCAGAGG CGACAGGACCTGTACAGTGCCCGGGACCTGCAAGGCCTCACTGTGGAACATGCCATCGATTCTTTTCGAG AAGGGGAGACTGTGGTCCTCACCCTCAAGGACAAAGGGGTACTGCAGGACGGGGAGGACGTGCTGGTGAA TGTGAACATGGTGGACAAGGAGCGGGCGGACAAGAACGTGGAGCTTCGGAAAAAGAAGCCTGACTACCTG CCCTATGCGGAAGATGAGAGTGTGGACGATTTGGCACAGCAAAAACCTCGTTCTATCCTGGCCAAATATG ATGAGGAACTGGAGGGCGAGCGACCACATTCCTTCCGTCTGGAGCAGGGTGGGATGGCCGATGGCCTGAG GGAACGAGAGTTAGAAGAAATCCGCACCAAGCTACGGCTGCAGGCTCAGTCTCTGAGCTCCGTAGGGCCC CGGCTTGCCTCTGAGTACCTCAGTCCCGAGGAGATGGTGACCTTCAAGAAGACCAAACGGAGAGTGAAGA AAATCCGAAAGAAGGAGAAGGAGGTGATAATGCGGGCAGATGACTTGCTGCCCCTGGGCGACCAGACTCA GGATGGGGACTTTGGATCCAGGCTCCGGGGCCGGGGTCGGCGCCGAGTTCCTGAGGTGGAGGAGGAGGCC CTTGAGGATGAGGAGAAGGACCCTGTGGCCCAGCCGCCACCATCGGATGACACTCGAGTAGAGAACATGG ACATCAGTGATGAAGAGGATGGGGGAGCTCTTCCGCCAGGGTCCCCAGAGGGGCTGGAGGAAGACGAGGC AGAGCTGGAGCTGCAGAAGCAGCTGGAGAAGGGGCGCCGGCTGCGGCAGCTGCAGCAACTGCAGCAGCTC CGAGACAGCGGTGAGAAGGTGCTGGAGATTGTGAAGAAACTGGAGTCTCGCCAGCGCGGCTGGGAGGAGG AGGAGGACCCTGAGAGGAAGGGGACCATCGTGTTCAATGCCACTTCTGAATTCTGCCGCACGCTGGGGGA GATCCCCACCTACGGGTTGGCTGGCAACCGCGAGGAGCAGGAAGAGCTCATGGACTTTGAAAGGGATGAG GAGCGCTCGGCCAACGGTGGCTCTGAATCAGATGGGGAAGAGAACATTGGCTGGAGCACCGTCAACCTGG ATGAGGAGAAGCAGCATCAGGATTTCTCTGCTTCCTCTACCACCATCCTGGATGAGGAGCCCATCGTGAA CAGAGGGCTGGCTGCTGCCCTGCTCCTGTGTCAGAACAAAGGACTGCTGGAGACCACAGTACAAAAGGTG GCCCGGGTGAAGGCTCCCAATAAGTCACTGCCCTCAGCAGTGTACTGCATCGAGGATAAGATGGCCATTG ATGACAAGTACAGCCGACGGGAGGAGTACAGGGGCTTCACCCAGGACTTCAAGGAGAAAGATGGCTACAA GCCTGATGTTAAGATTGAATACGTGGATGAGACGGGCAGGAAACTGACTCCCAAGGAGGCATTCCGGCAG CTGTCCCACCGCTTCCACGGAAAGGGCTCAGGCAAGATGAAGACTGAGCGGCGGATGAAGAAGCTGGATG AGGAGGCGCTCCTGAAGAAAATGAGCTCCAGTGACACGCCCCTGGGCACCGTGGCACTGCTCCAGGAGAA GCAGAAGGCCCAGAAGACACCGTATATCGTGCTCAGTGGCAGTGGCAAGAGCATGAATGCGAACACCATC ACCAAATGAAGCCGCCCTCCTCCCTGGCCCAGATGTAGTGTTAAATAAAGTTCCCTCCTTATTTTTCAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5097832 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5097832 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10803 same embryo
	EMAGE:10804 same embryo
	EMAGE:10801 same embryo
	EMAGE:10805 same embryo
	EurExpress:euxassay_003249 same experiment
	MGI:4827872 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source