EMAGE mouse gene expression spatial database

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EMAGE:10822

Elmod3 ELMO/CED-12 domain containing 3 ( MGI:2445168)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001218. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001218_01	euxassay_001218_02	euxassay_001218_03	euxassay_001218_04

euxassay_001218_05	euxassay_001218_06	euxassay_001218_07	euxassay_001218_08	euxassay_001218_09

euxassay_001218_10	euxassay_001218_11	euxassay_001218_12	euxassay_001218_13	euxassay_001218_14

euxassay_001218_15	euxassay_001218_16	euxassay_001218_17	euxassay_001218_18	euxassay_001218_19

euxassay_001218_20	euxassay_001218_21	euxassay_001218_22	euxassay_001218_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10822_wholemount_strong.wlz

10822_wholemount_moderate.wlz

10822_wholemount_weak.wlz

10822_wholemount_possible.wlz

10822_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10822_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 12 13 14 16 17 weak expression: see section 11 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4182
Entity Detected:	Elmod3, ELMO/CED-12 domain containing 3 ( MGI:2445168)
Sequence:	sense strand is shown >T4182 CCCACCTGAACATGAGTCATGAGTGAAACATCTTGCTCTTTTTTTATTGAGAAAGAATTTCAAGATGGGC AGCTAGAAAATGTCTCTGCTGGGCTCTCTTCATCGTACAAGGACAAGGGTGCACTCATGGCTTTCAGAGG AATTCCTATCTCCGAGCTGACGAACCATGGCATTCTCCAGGCCCTGACGGCAGAAACCAATGGGTGGCAG CCAGGTGTTGTGAGTGAAGAGGTCCTCAGAGCCCAGGAAGAGTGGGAAGTGGTGGACACCATCCATCCAG ACATAGAGAGTGGAGTCCACTGCCAGCAGCCTGGCCAGCTCATCTCCTTCAACGAGGCCCTGGAGCACTT CCAGAGTGTGGACCTTTCCTCTTTCAAGAAAAGAATCCAGCCCACTATTCAAAGGACTGGGCTCGCCGCC CTCCGGCATTGCCTTTTCGGGCCGCCAAAGCTGCACCAGGGTCTTCGGGAAGAAAGAGACTTGGTTCTCA CCATTGCTCAGTGTGGCCTGGACAGCCAAAACCCAACGCATGGCCGAGTGCTCCAGACCATCTACAAGAA GCTGACTGGCTCCAAGTTCGACTGTGCCCTTCACGGAGACCATTGGGAAGACCTGGGCTTCCAGGGAGCA AACCCAGCCACAGACCTCCGGGGGGCAGGCTTCCTGGCCCTCCTGCACCTACTCTACCTGGTCATGGACT CAAAGACGTTTCTGATGGCCCAGGAGATCTTCCGCCTGTCTCATCACCATATCCAGCAATTCCCCTTCTG TTTGATGTCTGTGAACATCACCCGCATTGCCATCCAGGCCTTGAGAGAGGAGTGTCTCTCCAGGGAGTGT AATCGGCGGCAGAAGGTCATCCCGGTGGTGAACAGCTTCTACGCGGCTACTTTCCTTCATCTGGCTCGCG TGTGGAGGACCCAGCAGAAGACTATTTTAGACTCAGGCTTTGTCCTCAAAGACTTGGAAGCGTTGGCCAA GAAGAGCCCAAAACGACTGCTCAAGACCCTGGATGACTACCTCGCCCAGGTGTCAAAGGGACAGACCTCC TTGTTGGAGGCACACAAGCGCCCTGGATCACAGGGACCTCACTCCAGGGATCTCACTTTCACAGGCGTGT GTGACCTGCAGTCTCACTCATTCGAGAGTGCAGGGTTGATCTGACCCACCACGGAGAGACCAGGACAGGC TGGGTCGCTGGGGCTCTCTGAACTAAAGAATCATGTCTGGGCCAAAGCCCTTCGTGCCCCTGCAGATGAG ACTAGGAGGCTCCCGGGTGTGTGTAGTGACACCCACGCCCACATTTCTACATCAGCAGAGCACATTCAGG CAGCTAGAATTACCCCCCCATGCCGTCCTCCAAGCACGGAGTCTTCTGTCCTTCCCAGCTGGACTCCACT TCACAGGGTTGGACTCCTGATATCTCAGTGGAACTGAACAGAGCACAGGGCCAGACAGCCCTATCAGAAT TGCCTACACGTCTGCACCTAGAGGCAGAGACCCTGGAAGCAACAGCCAAGATTCTGACTCCCAACAGCTG TCCCCAGTGTCTGTGGTTGGAATTGGAAGGACAGAGACAGCAATATCTTCAGCCATCAGGGGGCAGCACA ACACAGAAAAAGTGACTGATAGGCAGGTCCTCTCTTCCTCCAGCCACCGATTTTTCAAGACACTTGTTTC CACCCTACCCTTCTCCTCTTTCCCCTCCCTCTCCTTTGCCCACCATTGTTTGTAATCTAGAAATGGGTAC TCGGCTGGCTAGACTAGTCATTGAGCGTCCCACCCCCAACGCCCACAGGCTGGAGCGCCTATGGAGACCA CTACCTATCTACCCTGGCTGAGAGGCAAAGGAAACTCACCCACCAGCAGTTTTAGAATAAAGGAATTGTT GTAATCTCTGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4487200 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4487200 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10819 same embryo
	EMAGE:10820 same embryo
	EMAGE:10821 same embryo
	EMAGE:10818 same embryo
	EurExpress:euxassay_001218 same experiment
	MGI:4824530 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source