EMAGE mouse gene expression spatial database

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EMAGE:10824

Tmprss4 transmembrane protease, serine 4 ( MGI:2384877)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001224. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001224_01	euxassay_001224_02	euxassay_001224_03	euxassay_001224_04

euxassay_001224_05	euxassay_001224_06	euxassay_001224_07	euxassay_001224_08	euxassay_001224_09

euxassay_001224_10	euxassay_001224_11	euxassay_001224_12	euxassay_001224_13	euxassay_001224_14

euxassay_001224_15	euxassay_001224_16	euxassay_001224_17	euxassay_001224_18	euxassay_001224_19

euxassay_001224_20	euxassay_001224_21	euxassay_001224_22	euxassay_001224_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10824_wholemount_strong.wlz

10824_wholemount_moderate.wlz

10824_wholemount_weak.wlz

10824_wholemount_possible.wlz

10824_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10824_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

weak

regional

weak expression: see section 12 13 14 15 16 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 14 15 16 weak expression: see section 07 08 09 17 18 19

anal canal

moderate

regional

moderate expression: see section 13 14

pharynx

weak

regional

weak expression: see section 11 12 13 14 15

esophagus

weak

regional

weak expression: see section 13 14 15

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08 09 weak expression: see section 10 11

hindgut

weak

regional

weak expression: see section 13 14

midgut

moderate

regional

moderate expression: see section 05 06 07 08 09 weak expression: see section 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4698
Entity Detected:	Tmprss4, transmembrane protease, serine 4 ( MGI:2384877)
Sequence:	sense strand is shown >T4698 CCACGCGTCCGGGCAGGTGGTAGCTGGCTTGCGGAAGGAAGAGGAAGAAGAGAATGTGGGCCATCAAGGA GCAAGGCCAGCCTTGCACTTGGGCCCCCTCTGCTCAGTGCTGACCAGGGCTTTCTGAGCCGCTTCCTAAT GAGGCTCATTTGAAGACCCCCCCACCCCCCTCCTGCTGTCTTGGGTGGCAGAGCTAGCTCCAGGCTGTAA GAAAATTAGGAGGATTACCAAAGCAGTATGGAGTCAGACAGTGGCCAACCCCTCAACAACCGTGATATTG TTCCCTTTCGCAAACCCCGAAGGCCCCAGGAGACCTTCAAAAAGGTGGGGATCCCCATCATTGCAGTGCT GCTGAGCCTGATAGCCCTCGTGATTGTGGCCCTTCTCATCAAGGTGATTCTGGATAAATACTACTTCATC TGCGGCAGTCCCCTGACCTTCATTCAGAGGGGCCAGTTGTGTGACGGCCACCTTGACTGCGCCTCAGGGG AGGATGAGGAACACTGTGTCAAGGACTTCCCTGAAAAGCCCGGAGTGGCAGTCCGGCTCTCCAAGGACAG ATCCACCCTGCAGGTGCTGGATGCAGCCACAGGGACCTGGGCCTCAGTCTGTTTCGACAACTTCACAGAA GCACTGGCCAAGACAGCCTGCAGACAGATGGGCTATGACAGCCAGCCCGCTTTCAGAGCAGTGGAGATCC GTCCAGATCAGAACCTCCCTGTTGCTCAAGTCACAGGAAACAGCCAGGAACTTCAGGTGCAGAATGGAAG CAGATCCTGCCTCTCAGGCTCCCTGGTTTCCTTGCGCTGCCTTGACTGTGGAAAGAGCCTGAAGACTCCT CGTGTGGTGGGTGGGGTGGAGGCCCCTGTGGATTCTTGGCCGTGGCAGGTCAGCATCCAGTACAACAAGC AGCATGTCTGTGGTGGGAGCATCCTGGATCCCCACTGGATCCTCACAGCAGCCCACTGCTTCAGGAAGTA TCTTGATGTGTCAAGCTGGAAGGTCAGGGCAGGCTCAAACATACTGGGTAACTCTCCATCCTTGCCTGTG GCCAAGATCTTCATCGCTGAACCCAATCCTCTGTACCCCAAAGAGAAGGACATTGCCCTTGTTAAGCTGC AGATGCCACTCACATTCTCAGGCTCAGTCAGGCCCATCTGCCTGCCCTTCTCTGATGAGGTGCTTGTCCC AGCCACACCAGTCTGGGTCATTGGATGGGGCTTTACAGAAGAAAACGGAGGAAAGATGTCTGACATGCTA CTGCAGGCATCAGTCCAGGTCATCGACAGCACACGGTGCAATGCAGAGGATGCCTACGAAGGGGAAGTGA CCGCTGAGATGCTGTGTGCAGGTACCCCACAGGGTGGCAAGGACACCTGCCAGGGTGACAGTGGTGGGCC TTTGATGTACCATTCTGACAAGTGGCAGGTAGTAGGCATCGTGAGCTGGGGCCATGGATGCGGCGGCCCA AGTACTCCTGGAGTGTATACCAAGGTCACTGCCTATCTCAACTGGATCTACAATGTTCGGAAGTCTGAGA TGTAACGCTGCCGTCCCCCACATCCAGAAGCTGCTTCCCTTCAGACCTACCTACGGCATGACCCCTCAAA GTCAGATATGGGACAAGAGCCTCCTTGAACAAACTCTGGTATCCCTGCAGCAAGCAAGGATACATTGCAG AGGTGCCCGGAGTGGAGTCAGATGGGCTAGCTCAGCCACCCCTGCATCTCCCAAACCCTGGGAGACATGT GGCCCATGGGAGTAAATCCAGGACATTGACTCAACTCTCAGAAGTGTTATTCAGTCAAGGAGGCTCTCCC TTCCACTGAAGGAAGGAAAGTCAGCTCTCTCCTGAAAGGCCAGATCACTGGCTGAGTAGATGAGACAAGG GTATGAAAGGCCTTTGCCATCTTCTTTGCCCAGTCCTGAAAGCACTGACGTAAGAGACCAGTCAGTTCTA ATGTAAGGTGTATATTTTAGTCTCAGGGTATTGCAATTGTCACCCCTGTGGTCAATATCATTAAACAGGT ATGAGAATTCGCTGGCATAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5030266 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5030266 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10826 same embryo
	EMAGE:10823 same embryo
	EMAGE:10825 same embryo
	EMAGE:10827 same embryo
	EurExpress:euxassay_001224 same experiment
	MGI:4828830 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source