EMAGE mouse gene expression spatial database

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EMAGE:10867

BC031781 cDNA sequence BC031781 ( MGI:2384788)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003360. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003360_01	euxassay_003360_02	euxassay_003360_03	euxassay_003360_04

euxassay_003360_05	euxassay_003360_06	euxassay_003360_07	euxassay_003360_08	euxassay_003360_09

euxassay_003360_10	euxassay_003360_11	euxassay_003360_12	euxassay_003360_13	euxassay_003360_14

euxassay_003360_15	euxassay_003360_16	euxassay_003360_17	euxassay_003360_18	euxassay_003360_19

euxassay_003360_20	euxassay_003360_21	euxassay_003360_22	euxassay_003360_23	euxassay_003360_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10867_wholemount_strong.wlz

10867_wholemount_moderate.wlz

10867_wholemount_weak.wlz

10867_wholemount_possible.wlz

10867_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10867_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 17 weak expression: see section 07 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6745
Entity Detected:	BC031781, cDNA sequence BC031781 ( MGI:2384788)
Sequence:	sense strand is shown >T6745 AGAGGTTCCGTCACCAGACGAAATGGCGGAGGCTGCGGTGGTGGCGTGGGTTCGCGGCCCCGGTACCGTG TGGAAGGCGCTGCCGTGTGCCTCGGTTGGGTGCTCCGTGCGCGATGTGATCTACCGACACTGCCAGGAAC AGGAAGTTCCCGTGGAATGCTTCTTTGTGACGTGCAATGGAGTCCTCGTTAATGCCGGTGATAAGGTGCA GCATGGAGCCGTGTACAGTTTGGAGCCCAGGCTTCGTGGGGGGAAAGGAGGTTTTGGGTCTATGCTTCGA GCACTTGGTGCACAGATAGAGAAGACAACCAATAGAGAAGCCTGCCGGGATCTCAGTGGGAGGAGATTAC GAGATGTCAATCATGAGAAAGCGATGGCTGAGTGGGTAAAACAGCAAGCTGAGCGAGAGGCTGAAAAGGA GCAAAGGCGCCTGGAGAGGCTGCAGCGAAAGCTTGCCGAGCCTGCACACTGCTTCACCAGTCCCGACTAC CAGAGGCAGTGCCATGAGATGGCTGAGCGTCTGGAGGACTCAGTCCTCAAAGGTATGCAGGCTGCATCCA GCAAGATGGTATCAGCAGAAATCACTGAGACTCGGAAACGGCCTAACAAGTCAAAAACGGACCAGGAAAC TAGTGCAAAAAAAAGGAAAAGGAAATGCTTTTGGTTGGGCATGGATGGGCTGGAGGCTGCCGAGGGGTCC AGTACTGGAAGCTCAGAGGACAGCTCAGAGGATGACAGTGAGGATGCTCCTGGGACTTCGGAGCAGAGCT GCTGTGCTCGGGAAGACGGCATTGATGCTGTAGAGGTGGCAGCCGACCGACCTGGCAGTCCTCGGAGCTC GGCCTCTGGTACACACTCTGAGTCACCTGAAAAGCTTCAGTGCCCCGTGACCGAGCCTGGGCAGGGCATT TTGGAGAACACAGGAACTGAGCCAGGAGAGACCTCTGACAAGGAGTGCAACGAGAGGAAGACAGTCACAG ACCCAGAGGAGACGCCAGCAAGGAAGGAAACAGAGAGTCATGAGGCCACAGAAAAGGACCAAAAGACAGG AATGTCTGGTGGGGACAGAGCTGCCATGGTTTTGTCTGGAGAAGACAGGAAAAGTGTCCCTGCTGCTAAT CTGGAGGGAAACAACTCAGGAGACACAGCTCTTGGTCTGGAAGCGGTGGATCTGTCAGCATTCAGCTCTG CAGCCGAGTTGGAGTCGCTGGGTTTGGAGAGGCTCAAGTGTGAACTGATGGTCCTTGGACTGAAGTGTGG GGGGACCCTGCAGGAGCGCGCAGCCAGGCTCTTCTCTGTCAGAGGCCTCACAAAGGAACTCATTGACCCA GCTTTATTTGCCAAGCCTTCCAAGGGGAAGAAGAAGTGAATTTCCTTGGCACACTTCCTCCGCTGTGACA GACTTGTGGCCTGTGTTCCTTTTGATACTAAAAGCCAATTTTTTAATAATTCGGTTCTAACTAGTCTCTT TTCAAACTAGTCTCTTTTCAGTGGTACCTGAACTTGAGGTTTTCGTTGGAGGAGAAAACTTTTGTATGTT ATTTTTCTTTGTTTTTGATGTTTTTGTAAGAGATACTCTGTAGCCCAGACTGACCAAGAACTGTGTGGTT CACTTTGGTCTCAAACTTGCAGTGATCCTCCTGTCTCGCATGAGCTGCTGTGCCTGCTTTGTCAGAGGTT TATACATTTCTTTGGCTTTATATACTGTGTCACTTGAGTAAGATATTTTCAAGAAATTTATTTGTATAAC CCAAAGCAGTCAGGTTTGTAGACACAGATATTCATAACATTCATTTTAAAAACTTGTTTCTCTTTCTAGG AGAGAGAAAGAGAGAGAATGAGAATATGAATGTGTGTGTGTGTGTGTGTGTGTGAGACCAGGGATGGAGC CTAGCTGCTTCCAGGCCTCTCACTGGGGATTTTTATTCTAGCTAGGGACCTATTTTGTTTGGTTTTCAAA GAATCAGTTTTTTGTCTTTGTGGATTTTTCTCAGTTGTTGATCTCATTGGCATTTGAATTGTATTTCCTC TCCTACATGCCTTGTGTTTATCATTTTTCTTTAGCTCCTTAAGTCTTTGATCACTGATTATAGATTTTAT TTTTATTATTATTAGTGTATTGCCCAATTAGCAAATGTTTGTGTTTTCTGTAAATGTTTTATTGATAGTG ACTCTCTGACTATTCAGGACAGAAAGTACCTGGCATCCTTGTTGTGACTGACCCAGGATAGGGTCTGACA TGATCTGTGCTGTGTGTGTTGGAAAGAACGTACCATCAGCCGGCAGCAGGGTGGGTTCTGGACACCTATC ATGTGAGTGGTTGGTCGGATCGGTCAGATGACCGCCTTCGCTGGTTCATTTTCATAAGGAACTAAGAGCA AAATCAAATCTCAATTAGGATTGTGGCAATTGCCTTTTTTGAGATTAAAAATAACTTCATCAGCGTTTGC TAAGTTGTCAGTTGTATATTAATTCAGTGACTTTATATGTCTGGCTCTGGGCTGGAGAGACCGCACTGAC TGCTCTTCCAAAGGTCCTGAGTTCAGTTCCCAGCAACCACATAATGGCTCACAACCATCAGTACAGCTAC AGTGTACTCATATACTATACATAAAATAAATAAGTAAATAAATCTTTTAAAAAATATATGGCTCTGATGG GTTTTGGATTTTTTTCCCCATCTGACAGTTCACGCATGGACACTGCGTCCTGATTAGGTCCACAGTTTCT ACTTCTCTAACTCTTCTGGTTTACTTTATATGTTAAAGCTACTGTTCAGTTCTTACACTTATTGAACATT ACATCATGAAATTCCCTTCATCTCTGGTGAGTTTCTTGCTCACCTGTATTATCTAAAGCACAGCTTTGTC CTTGTTTCCACAGTGCGTTTTCTGTTCCTCTCTCCTTGCTCCTCCTGTCTAACACACAGCAGGTGTGTCT CGCATCTGTGTCTGACTTCTTGTCATCATCATCACTGTCATAGCCATGATTGTCATTGTCATCATCTTCA TCTGGTCCCTGTCTCTGATGACAGGTCAGCAGTGAGTCACATGGTTGTTTCGGTCATAACATTTTCTTTG GGGTGTTTTCAGGTGTGCCTAAATGTCTACTCTTCTGCTCTGGAGTTTGGTGAACTTCATGGTTTTGTAT ATTTATTCCATTAATAAATTTGGAAAAAATTTTAAGCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3672316 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3672316 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10868 same embryo
	EMAGE:10865 same embryo
	EMAGE:10866 same embryo
	EMAGE:10864 same embryo
	EurExpress:euxassay_003360 same experiment
	MGI:4823424 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source