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EMAGE:10876

Rhbdf1 rhomboid family 1 (Drosophila) ( MGI:104328)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10876 EMAGE:10876 EMAGE:10876 EMAGE:10876 EMAGE:10876
"Pseudo-wholemount" of euxassay_003347. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003347_01 euxassay_003347_02 euxassay_003347_03 euxassay_003347_04
EMAGE:10876 EMAGE:10876 EMAGE:10876 EMAGE:10876 EMAGE:10876
euxassay_003347_05 euxassay_003347_06 euxassay_003347_07 euxassay_003347_08 euxassay_003347_09
EMAGE:10876 EMAGE:10876 EMAGE:10876 EMAGE:10876 EMAGE:10876
euxassay_003347_10 euxassay_003347_11 euxassay_003347_12 euxassay_003347_13 euxassay_003347_14
EMAGE:10876 EMAGE:10876 EMAGE:10876 EMAGE:10876 EMAGE:10876
euxassay_003347_15 euxassay_003347_16 euxassay_003347_17 euxassay_003347_18 euxassay_003347_19
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euxassay_003347_20 euxassay_003347_21 euxassay_003347_22 euxassay_003347_23 euxassay_003347_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10876Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10876_wholemount_strong.wlz
10876_wholemount_moderate.wlz
10876_wholemount_weak.wlz
10876_wholemount_possible.wlz
10876_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10876_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6350
Entity Detected:Rhbdf1, rhomboid family 1 (Drosophila) ( MGI:104328)
Sequence:sense strand is shown

>T6350
TCCGGGAGCTGGACCTGCCCAGCCAGGACAATGTGTCCCTAACCAGCACCGAGACACCACCTCCCCTCTA
CGTGGGGCCATGCCAGCTGGGCATGCAGAAGATCATAGACCCTCTGGCCCGAGGCCGGGCCTTTCGCATG
GCTGATGACACTGCTGATGGCCTGAGTGCCCCCCACACTCCCGTCACACCAGGTGCAGCCTCCCTCTGCT
CTTTCTCCAGCTCCCGCTCAGGTTTCAACCGACTCCCTCGGCGTCGCAAACGTGAATCGGTGGCCAAGAT
GAGCTTCCGGGCCGCTGCAGCATTGGTGAAGGGTCGGTCTATTAGGGATGGCACTTTACGAAGGGGACAG
CGCCGAAGCTTTACCCCTGCCAGCTTTCTTGAGGAAGACATGGTGGACTTCCCTGATGAGTTGGATACAT
CTTTCTTTGCTCGGGAAGGTGTCCTCCACGAGGAGATGTCCAC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGACATCCATCACACAGAC; Reverse Primer - name:unspecified, sequence:GTGAGCCAGTAAGTGAAGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10878 same embryo
 EMAGE:10875 same embryo
 EMAGE:10877 same embryo
 EurExpress:euxassay_003347 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS