EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10880

Smg6 Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) ( MGI:2144117)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003343. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003343_01	euxassay_003343_02	euxassay_003343_03	euxassay_003343_04

euxassay_003343_05	euxassay_003343_06	euxassay_003343_07	euxassay_003343_08	euxassay_003343_09

euxassay_003343_10	euxassay_003343_11	euxassay_003343_12	euxassay_003343_13	euxassay_003343_14

euxassay_003343_15	euxassay_003343_16	euxassay_003343_17	euxassay_003343_18	euxassay_003343_19

euxassay_003343_20	euxassay_003343_21	euxassay_003343_22	euxassay_003343_23	euxassay_003343_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10880_wholemount_strong.wlz

10880_wholemount_moderate.wlz

10880_wholemount_weak.wlz

10880_wholemount_possible.wlz

10880_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10880_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6188
Entity Detected:	Smg6, Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) ( MGI:2144117)
Sequence:	sense strand is shown >T6188 CGGACGCGTGGGGCAGAGGCTTGTGAAAGGGAGGAGAGCAGCCCAGGAAAGGCTAGAAACAGACCATTGG GAACCCTAGGATGGAGACATTCCCTGCAGTGGCTGAGAAGGTCCTTAAGGAGTTCCAAGTATTGCTGCAG CATAGCCCATCTCCTATTGGAAGTACCCGTATGCTGCAGCTTATGACCATCAACATGTTTGCCGTGCATA ACTCCCAGTTGAAAGGTAAGGGAAATCTAATTTCTCCATCTAGACAGAGTTGAATTTAGTATCTTTGCTA CCTTATCACTAACCCCATCTCCACACACACAGACACACAACACACAACACACAACACACACACACACACA CACACCTCTCCCTTCTATTGCTTCTTAGATCTTTATTTTAGTCTCTAAATTCTGTAAGACTCTATAGTGC TTGGTTGGGATATTAAGGAGAGATTTGTAAATATAAAGTTTAATTTAAAAATCCAGAGATGCTTTTGCAC ATCTGGCTATGCTAATGGCAGCAGCAAGGCCTCTTAGAGGCTGCATACCCAGGACTTCTCCCAAGAGAGA GGCATTGCTGAAATGTTTCATTCCAGAGACCGTATGCTTGGCAGGGATTAGGCATTGCTGTTTCTGGACT CGTGTACCCTGTTTGTTCAATATGGAAAAGTCTAGTTCCCTATGGGATATCCCTTATGGGACCCTGCCAG AGAGTAAGAACAGAGAATTGATGAGCTTTGGTGACTCAAATCTGGAAGCACAAAACCCACCAGGCCCAGA ATCTGGCCAGTGTACTGGTATGCGTTCTGAAGTGTTTTTCTGAACACTTCTCCCCACACCCCCGTATGAG GCGGGAGGCCAGGAGGGAAGGGCAGATTGGCAGAATTTTAAAATGTTCTGATGACCATGCTGCATTTGAA GGATGTTTCTAGGATCCCTTCCCCTCCCTCTTCCTCACTTAGGTAAGGCTGATGTGGTTTGACTTGGTAG AAATATGTAGTCTGAGTGACATTATTTTGTGTTTCTTTACACCATGTTGCCAAGGAGTCATTCCAGAGCA TCAACAGGGAATGTTTGCTCAGATTCTTATCTGCAGTGCCAGGAAGTAGGAATTGCCCCATGGAGACTGT GTCACAAAGCAGATGTAATGTCTCTGGACAGTGCCACTGGCTCTTCAGTTCACCTTTTCTCAGAAATTTC TCCTCTCACCTGCACTTGATCTGCTTCCCTTCCTGTCCATATTAGGATCTATGTTCAACTTGCTGCTGCT AGGGAACTTCCGTAATGAGTGAGCCTGCCGAACTTTCCCTATGGACAGGGCTTCTCTTGGGATTTGAGGA GCTTCTTAGCTGGTCTGCCTGGGTTTTCAATCATATCAGTCCTGGAAGACATTTCCTCATCAGGTTGACT TATACAGCATCCAAAATGTTCAGCAATGGGCAAGATACAGAGGCTTAGGAGCCTTCACAACTTAGCCAAT TTTTTGCTGAGGTGGCTGTTTGCTGAGGTGGGGTTGCCAGAGGCCTGGAGCCTTAAGGGACAGCACTGAT TTCTAAGGAGAAGGAAATAAATAAGTTTGTAAAATGCTGCCCTAGTGAGGCTGTGCAGTCCTAAAGGGAG GGAAATTTTGCTACCAAGAATGAAGCTCTCAGGGTGTGGTGGCACATGCCTTTAATTTTGGTCTACTTAT CGAGTTCCTGACTAGCCAGAACTACATAGTAACACTGTCTGCTCAATGGGCTCTGTAAGTAGGGCCTCCA GTTGCCCCTGTATTCATTTTCTGCTTTCCTACCCAAGCCACCACTGCCGTGTGAGCTGGAGAGCTGGGAC ACACAGTTCAGCAACAACTGACATATGGTCTCTATAGATGTGAATATAAAGAACCTGGTTTCAACACTTT TGGTGGAAAGGAGTACCCTAGACTTTCATAGACCACCAGTGAGTCTTTTTCTTGTAGCTGTTAAGTAAAG GTTAAGAAAATAAGGGTGTTTGATATCAGTGGGAGGAACAGCCCTTGGGCCTGAGGGGGTTCGATATCCC AGTGTAGGGGAATGCCAGGGCGGAAAGGTGGGAGTTGGTGGGTGGGTAGGGGAGCACCCTTATAGAGGCA GGGGGGAGAGAGAATGGGATAGGAGATTTCTAGGGGGCAGGGGGAGACCTAGGAAGGGGGTAAAATTTGA AATATAAATAAAGAAAATATCCAATAAAAGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497983 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497983 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10879 same embryo
	EMAGE:10882 same embryo
	EMAGE:10881 same embryo
	EurExpress:euxassay_003343 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source