EMAGE mouse gene expression spatial database

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EMAGE:10893

Fam84a family with sequence similarity 84, member A ( MGI:2145011)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003388. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003388_01	euxassay_003388_02	euxassay_003388_03	euxassay_003388_04

euxassay_003388_05	euxassay_003388_06	euxassay_003388_07	euxassay_003388_08	euxassay_003388_09

euxassay_003388_10	euxassay_003388_11	euxassay_003388_12	euxassay_003388_13	euxassay_003388_14

euxassay_003388_15	euxassay_003388_16	euxassay_003388_17	euxassay_003388_18	euxassay_003388_19

euxassay_003388_20	euxassay_003388_21	euxassay_003388_22	euxassay_003388_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10893_wholemount_strong.wlz

10893_wholemount_moderate.wlz

10893_wholemount_weak.wlz

10893_wholemount_possible.wlz

10893_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10893_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 16 17 18 19 weak expression: see section 08 09

vibrissa

moderate

regional

moderate expression: see section 02 03 04 05 18 19 21 22 weak expression: see section 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10 11 13 14

urethra of male

moderate

regional

moderate expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5988
Entity Detected:	Fam84a, family with sequence similarity 84, member A ( MGI:2145011)
Sequence:	sense strand is shown >T5988 CAGGACTTGCCAGGTGGAGCAGACGCCCAAGCCAGCGTAGACACGGAGCGCATCCGCACCGCTCTGGGTA CCTCACCTCACTGCTCGTCTCCGGATAGATCGGAACCGCAGTCCCACGCGACTCCAGGACCCACTCGCCA CTGCCGCCTCCACAGCACCCATGGGGACCTGGAGACTTTAAAAGGAGTTTGGGGTTTCGGGCGCAGGGAA ATCACGGATCTCAGTTGCTGGCCCTGGCCTCGCCGCGTCATTGATGGGCAACCAACTGGACCGCATCACC CACCTCAACTACAGCGAGTTGCCCACTGGGGACCCATCTGGGATTGAGAAGGACGAGCTGCGAGTCGGGG TTGCCTACTTCTTCTCGGATGAGGAGGAGGACCTGGACGAACGCGGGCAGCCGGACAAGTTTGGTGTGAA GGGCCCCCCAGGTTGCAGCCCTTGCCCAGAGAGCCCCAGCCGCCACCACCACCACCTGCTGCACCAGCTC GTCCTCAACGAGACTCAGTTCTCCGCCTTCCGGGGCCAGGAATGCATCTTTTCCAAAGTGACCGGCGGCC CTCAGGGCGCCGACTTGAGTGTCTACGCGGTCACTGCGCTGCCGGCGATCTGCGAGCCAGGCGACCTGCT AGAGCTCTTGTGGCTACAGCCGGCGACCGAGCAGCCCGCGCCAGCCCCTCACTGGGCCGTCTACGTGGGC GGCGGGCAGATCATCCACCTGCACCAAGGCGAGATCCGCCAGGACAGCCTGTACCAGGCGGGCGCGGCCA ACGTGGGCCGGGTGGTGAATAGCTGGTACCGCTACCGCCCGCTGGTGGCCGAGCTAGTGGTGCAGAACGC CTGTGGCCACCTGGGCCTCAAGAGCGAGGAGATCTGCTGGACGAACTCCGAGAGCTTCGCTGCCTGGTGC CGCTTTGGAAAGCGCGAGTTCAAGGCTGGCGGGGAGGTCCCCGCAGGAACGCAGCCGCCGCAACAGCAGT ATTATCTCAAAGTGCATCTGGAGGAGAACAAAGTCCACACGGCCCGGTTCCACAGCCTGGAGGACCTCAT CCGCGAGAAGCGTCGCATAGACGCCAGTGGCCGCCTGCGAGTGCTGCAGGAGCTTGAGGACTTTGTGGAC GACAAGGAGTAGCCTCAGAGGGGCAGCTCGGCGTGCCAGTTCCCACGCCCGGAGTTCCCTTTCGGTCCCC GCAGTTCTGCACCCCATGTTCCCCTAACGCGCCCGCCCAGGGCCAGCGGCTGGAAGCTTCGGGAACTCAC CACCACCCACCGCACTGGGCGGGAGCAGCTGCGCGCTCCTAAGACAACCCTGGTGTTGGTGATCATGCCA GGGTAATCCAGCCTGCGCCTCTGCCCTGGGTTGTGAAGGGAAAGAGGGAGTGGGCAAAAGGGGAGCAAGG ACACTACCTTTCCAGGGAGCCAAGGGTCAGTGACTCTTCTGCAGACAGGGCAAATATCCTTAGTCACAGG GAACCGTCACCCACCACCCCCAGATGTGCACACACAAACCAGTCGGAGGTGAGAGTTGGTCTTTTCAGGA CACAATTCAACTCCCGAATGGAGAGTGGATGTGCCCCCGGATATCAGAATTCCCATGAAACCGAGCCTGT CTCTGTACTTTGGAGCACTTCAGGGACTCCAGGTTACCCTGAGGCTGCCTGGAACTGTCTTGCAGCTCTC CAGCCCAGAGTCTCCTGACAAAGCATTCCAGGCTGAGCTAGATAGGATTGACTTCTCAGTTTGAGCAACT GGAAGCCGATGCCATTCCCGGCTTGACCGACACTTGCCAGAAGGAAAGCCTTCTGGGTCCCGGGAGGAAA ATGGTTGAAGCACCTTTTTGGGAGAGGAGGGCCTGCTGGCTCCGGAAAAGGAGCTAATTCACCTGGCCTG TGGGCACTTGAACTTAGGAAGGAGGAGGCTTTTTGCTCGGAAGCCCTTGATTGCTTCCCTTCTCACTCAG AGTCCTGTATTCAGCGGGACTGAAGGAGAGGGAGGCAGGGAAGATCTACAACATTCCAGAAACCAGCATT AGGACAGCCTAGCCAGTGTCTTTAGTTTGGCTTTTCCTAACTAGAAGGCAGGAGGCAGGAGGCAGGTGGA GATTGTTTGTAAAATGAAACAGCAGTTTTCCAACGATATCAACAAAGCCTACAGTGTCGATGCTTCTCTT GATCATTTTTAGTAACATGCTAATGAAATTTCAAATTGAAATTTTTATTTTCATGGCTTTAATCCATGAT AGCTTAAATACTGGGGGCCATTAGGAGTGGAATTTAGCTAAGACCTTAGCTGACATTGCTTTCACTCTGG TTTTTCTCAACTCTTATGAGAATTCTGTTTGGGGATATTATAATTATTCCTTTTTTTTTTTTTTTTCCTG GCTTTACAAAGTAGGCCTGCAATGGTGGAGCTGTTTATTAGTGTGTATATTGTACTGAATTTTTTCAGTT CAAGGGTCTGCTGCTTTGGTGAAAATTGCTAGCAAGTTCCATGAGGGTTCTTTTCTTCATTCTGTTTACC ATCATCATCTCATCTTTGCATTCCCCCCTCTCCTCACCTCTTGATCCCCCTTGAAAGGAATTTAGAGTTC ATGGGCTTTGGGGCCCGCTTTTGTAGCTTGTCCTGATGTTTATTTCTGAGAACAGGGAAGCGCTCTTAGA AGCAGGACAGAATGTGTGTCTTTCTAAACATGAAGGTTATGTGACGCTCCTTTCTTTGGGCAAGAGTTTG TGGATTTTTCTCCAAGGGGTACAACCCCAGCCTAACTGATCGTTACTTGATTCTTTGTGTTACACACACC TTGGAAGGTGCACAGTCTCTCATGAGGGAGTTTGTCAAATAATGATGTTTAGCTCATGGTTGCAAGCAAT TGGATACTATGGTTTCATTGAACAGCAAACACTATGTCCAAAGCCACTTTCTTGGCATTTCAAAACTAAT GCAATAAAAACTAGTAGAAGTTAGCAGGCAGGGAAATGCCATTTCCATGGTAAATGGTTTGTTTCTGTTT TCTGGGGATTTTTGTTGTTTTATTTTGTTTCATTTTGTTCTCATGCTGGATTCATTCTCTGATCTTGAGT CAAAAGGTCAAATCAATGAAATATGAACTAAAGTATTTTTCTGAAGCCAATCGAGTGATTTATTTTTTAA AACTGCTTATGTTTTTTCTTTCAGCACATACAACAGCAAAACTTTTGTAAACTTAACCTTTGCATGTATG GAGAACTGAAAGTCATTTATTTCTCTAACTTAGTCTTTTGGCAAATGACAGATTGATGACCATAAACCGA ATTCTTTCTTGTGTCTACACACACCATATCCTTTCCTGTGTCCTACTACTGTATCTTGGCTCCCTGCTGA ATTAAATACCATCCTAAGCAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3486254 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3486254 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10894 same embryo
	EMAGE:10891 same embryo
	EMAGE:10889 same embryo
	EMAGE:10892 same embryo
	EMAGE:10890 same embryo
	EurExpress:euxassay_003388 same experiment
	MGI:4824769 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source