EMAGE mouse gene expression spatial database

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EMAGE:10910

Grwd1 glutamate-rich WD repeat containing 1 ( MGI:2141989)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006537_01	euxassay_006537_02	euxassay_006537_03	euxassay_006537_04

euxassay_006537_05	euxassay_006537_06	euxassay_006537_07	euxassay_006537_08	euxassay_006537_09

euxassay_006537_10	euxassay_006537_11	euxassay_006537_12	euxassay_006537_13	euxassay_006537_14

euxassay_006537_15	euxassay_006537_16	euxassay_006537_17	euxassay_006537_18	euxassay_006537_19

euxassay_006537_20	euxassay_006537_21	euxassay_006537_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10910_wholemount_strong.wlz

10910_wholemount_moderate.wlz

10910_wholemount_weak.wlz

10910_wholemount_possible.wlz

10910_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10910_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6770
Entity Detected:	Grwd1, glutamate-rich WD repeat containing 1 ( MGI:2141989)
Sequence:	sense strand is shown >T6770 GGCGCGCAAGGGCCGGAGTCGCACGTGTGAAACCGGAGAACCCATGGAGGCTGAAACCTGCGACCCAGGA ACCGAAGGCCCATCCCAGGTCTACCTGCCCGGCAGAGGGCCGCCACTGAGCGAGGGAGAGGAGCTGGTAA TGGATGAAGAGGCCTACGTGCTGTACCATCGTGCGCAAACTGACTGATGATGCTTCGGATGCACAATCTC CATGGCACCAGGCCGTCACCCTCAGAGGGCAGTGATGACGATGAGGAAGATGAAGATGAAGAGGATGAGG AGGAGCAGAAACCTCAGCTGGAACTGGCCATGGTTCCTCACTACGGTGGCATCAACCGAGTTCGGGTATC TTGGCTGGGGGAGGAACCTGTGGCTGGAGTATGGTCAGAGAAGGGCCAAGTGGAGGTGTTCGCACTGCGG CGGCTCCTGCAGGTGGTGGATGACCCCCAGGCCCTGGCCATCTTCCTCCGAGATGAGCAGGCCCGAATAA AACCCATCTTCTCCTTTGCTGGCCACATGGGAGAGGGCTTTGCTCTTGACTGGTCCCCTCGGGTGCCCGG CCGCTTGCTGACTGGTGACTGTCAGAAGAACGTCCACCTCTGGACACCCACGGAGGGTGGTTCCTGGAAT GTGGACCAGAGGCCCTTTGTGGGCCACACACGGTCTGTGGAGGATCTGCAGTGGTCACCAACTGAGGACA CGGTGTTTGCCTCCTGCTCTGCTGATGCCTCTATTCGCATCTGGGACATCCGAGCAGCCCCAGGCAAGGC CTGCATGCTCACCACAGCCACTGCCCATGATGGAGATGTTAATGTCATCAGCTGGAGCCGCAGGGAGCCC TTCCTGCTCAGCGGTGGGGATGATGGTGCCCTCAAAGTCTGGGACCTGCGACAGTTTAAGTCTGGCTCCC CTGTGGCCACCTTCAAGCAACATATGGCCCCCGTGACCTCTGTTGAATGGCACCCTCAGGACAGTGGGGT CTTTGCAGCCTCAGGTGCTGACAACCAGATCACACAATGGGACCTGGCAGTTGAACGGGACCCAGAGTCG GGGGAGACAGAGACAGACCCTGGGCTGGCGGCACTCCCCCAGCAGTTGCTGTTCGTGCACCAAGGTGAGA CTGACCTGAAGGAGCTACACTGGCACCCACAGTGCCCGGGGGTCCTGATCAGCACCGCCCTGTCAGGCTT CACCGTCTTCCGCACCATCAGTGTTTGAGGTGGCCAACACTTGGCCTCAAGACTTGGCTCCACATGGGAA CTGGATCGGTTAACTTGGCAAGGATCATTAGGCCTGTAGACTGCACCTCAGGAAGGAAGACTTGTTCAAC TGGCCTACGTGGAACAGAAGGAACGGACTCTACTGACCTGGTATCTCTAAGGATTTGGATTGACCCTTGG CTCTGCCTTACACCAAAGACTAGGTGCAGGCAAAGATCCTTGCCTGTCCCAGATACGGCCTCAGGATGAG ATTTTTGTCAGTGTTGACTCCTGGCTTGACCCCAAGGACCTCTGACCAGGGACCTAATTCTGGCTGTTGA TCAACTTCAGGAACTTTAAGGGTTTTGCCATAGCTGGGCCAAGTCTGGGTCTGTGTCACTCCAGTGGAAT TTAATTCTCTCTCTGCCTTCTGGCCAGTTAGATTTGGTTGAGATCCAAGTAGGGAGCCATCTGGGGTGCC CTCCTGTGCTGACCACTACTGTACTGGAGGAGGGTCTGGGGCTTCTGCTGTAATAAAGGACCACAATAAG GCCCCAGACACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3674738 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3674738 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10908 same embryo
	EMAGE:10907 same embryo
	EMAGE:10909 same embryo
	EurExpress:euxassay_006537 same experiment
	MGI:4825247 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source