EMAGE mouse gene expression spatial database

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EMAGE:10914

Prpf31 PRP31 pre-mRNA processing factor 31 homolog (yeast) ( MGI:1916238)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006548. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006548_01	euxassay_006548_02	euxassay_006548_03	euxassay_006548_04

euxassay_006548_05	euxassay_006548_06	euxassay_006548_07	euxassay_006548_08	euxassay_006548_09

euxassay_006548_10	euxassay_006548_11	euxassay_006548_12	euxassay_006548_13	euxassay_006548_14

euxassay_006548_15	euxassay_006548_16	euxassay_006548_17	euxassay_006548_18	euxassay_006548_19

euxassay_006548_20	euxassay_006548_21	euxassay_006548_22	euxassay_006548_23	euxassay_006548_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10914_wholemount_strong.wlz

10914_wholemount_moderate.wlz

10914_wholemount_weak.wlz

10914_wholemount_possible.wlz

10914_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10914_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6762
Entity Detected:	Prpf31, PRP31 pre-mRNA processing factor 31 homolog (yeast) ( MGI:1916238)
Sequence:	sense strand is shown >T6762 GCAGATTTCTTGGAAACGATTGTGAGAGGATCGAAGCGGCCTTGGGATGTCTCTGGCTGATGAGCTCTTG GCTGACCTCGAGGAGGCAGCAGAAGAAGAGGAAGGAGGAAGCTATGGAGAAGAGGAGGAGGAGCCAGCAA TTGAGGATGTACAGGAGGAGACCCAGCTGGATCTTTCTGGAGATTCAGTCAAGAGCATTGCCAAGCTATG GGACAGCAAGATGTTTGCTGAGATCATGATGAAGATTGAGGAGTATATCAGCAAGCAAGCCAACGCGTCG GAAGTGATGGGACCAGTGGAGGCAGCTCCTGAGTACCGAGTCATTGTGGATGCCAACAACCTGACTGTGG AGATAGAAAATGAGCTGAACATTATCCACAAGTTCATCCGGGATAAATACTCAAAGAGATTCCCTGAGCT AGAATCCTTGGTGCCCAATGCTCTGGATTACATCCGCACAGTCAAGGAGCTGGGCAACAGCCTGGATAAG TGCAAGAACAATGAGAACCTCCAGCAGATCCTGACCAATGCTACTATCATGGTTGTTAGTGTCACTGCCT CTACTACCCAGGGGCAGCAGCTGTCGGATGAGGAGCTAGAGCGGCTAGAGGAGGCCTGTGACATGGCACT GGAGCTGAATGCCTCCAAACACCGCATCTATGAGTATGTGGAGTCCCGGATGTCCTTCATTGCACCCAAC TTGTCCATCATCATTGGAGCATCCACAGCTGCCAAGATCATGGGTGTGGCTGGAGGGCTCACCAACCTCT CTAAGATGCCTGCCTGCAACATCATGCTGCTGGGAGCCCAGCGCAAGACCCTGTCTGGCTTCTCTTCTAC CTCAGTGTTGCCTCATACTGGTTATATCTACCACAGTGACATTGTGCAGTCTCTGCCCCCAGATCTCCGG CGGAAGGCGGCCCGCCTAGTGGCTGCCAAGTGTACACTGGCAGCTCGTGTGGACAGCTTCCATGAGAGCA CAGAAGGGAAGGTGGGCTACGAACTAAAGGATGAGATTGAGCGCAAATTTGATAAGTGGCAGGAGCCACC ACCCGTGAAGCAGGTGAAGCCCCTGCCTGCACCCCTTGATGGGCAGCGGAAGAAGCGAGGGGGCCGAAGG TACCGCAAGATGAAGGAACGGCTAGGACTGACGGAGATCCGGAAGCAGGCCAACCGCATGAGCTTTGGAG AGATTGAGGAGGATGCCTATCAGGAAGACCTGGGCTTTAGCCTGGGCCACTTGGGCAAGTCAGGCAGTGG GCGTGTGCGGCAGACCCAGGTGAATGAGGCCACCAAGGCCAGGATCTCCAAGACACTGCAGCGGACCTTA CAGAAACAGAGCGTGGTGTATGGTGGGAAGTCAACAATTCGTGACCGCTCCTCAGGGACTGCCTCCAGTG TGGCCTTCACTCCACTCCAGGGTCTAGAGATTGTGAACCCACAAGCAGCCGAGAAGAAGGTGGCAGAGGC CAACCAGAAGTATTTCTCTAGCATGGCCGAGTTCCTCAAGGTCAAGGGTGAGAAAAGTGGCACCATGTCT ACCTGAAGGAGCCCGCACTCCAGGTGACCATCCACTGAAAGGACATAAAGGACTGGAGCTTCTGAAGTGA CAGATATCCAGCCAGGAAGTTGTGGTGTGGTGGAGGCAGCCTTCCATGCTTTGCCTTTGGCCCAAGGCCA TAGCATCCAAGGAAAAGGATGAAGAACCTTAGCCTGCCTCCCCCAGCATCCATGCTGCTGCTTCCCTAAA CGGGCAGAAAGGTCTTCCTCACACTCAGCTTTTTTACTTACAGGGGGAAAGGCAGTGCTTTTATAGAACG AGTACAATTAAATGTGTAGTGTCGGGCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3673893 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3673893 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10918 same embryo
	EMAGE:10916 same embryo
	EMAGE:10917 same embryo
	EMAGE:10915 same embryo
	EurExpress:euxassay_006548 same experiment
	MGI:4827444 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source