EMAGE mouse gene expression spatial database

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EMAGE:10941

Dbn1 drebrin 1 ( MGI:1931838)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006495. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006495_01	euxassay_006495_02	euxassay_006495_03	euxassay_006495_04

euxassay_006495_05	euxassay_006495_06	euxassay_006495_07	euxassay_006495_08	euxassay_006495_09

euxassay_006495_10	euxassay_006495_11	euxassay_006495_12	euxassay_006495_13	euxassay_006495_14

euxassay_006495_15	euxassay_006495_16	euxassay_006495_17	euxassay_006495_18	euxassay_006495_19

euxassay_006495_20	euxassay_006495_21	euxassay_006495_22	euxassay_006495_23	euxassay_006495_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10941_wholemount_strong.wlz

10941_wholemount_moderate.wlz

10941_wholemount_weak.wlz

10941_wholemount_possible.wlz

10941_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10941_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6815
Entity Detected:	Dbn1, drebrin 1 ( MGI:1931838)
Sequence:	sense strand is shown >T6815 GGCGGCGCTAGGACCGAGCGAGCCGAGGGAGTGAGCCGGGCGGAGCCAGCGCCCCCCGCCCCCCGCCCCC CGCAGCATGGCCGGCGTCAGCTTCAGCGGCCACCGCCTGGAGCTGCTGGCGGCGTACGAGGAGGTGATCC GGGAGGAGAGCGCAGCCGACTGGGCTCTGTACACATACGAGGATGGCTCAGATGACCTCAAGCTTGCAGC GTCAGGAGAAGGGGGCTTGCAGGAGCTTTCCGGCCACTTCGAGAACCAGAAAGTGATGTATGGTTTCTGC AGCGTCAAGGACTCCCAAGCTGCCCTGCCAAAATATGTGCTCATCAACTGGGTTGGTGAGGATGTGCCTG ATGCCCGAAAATGTGCTTGCGCCAGTCATGTGGCCAAGGTGGCTGAATTCTTCCAGGGTGTTGATGTCAT TGTGAATGCCAGCAGTGTGGAAGACATCGATGCTGGTGCCATTGGGCAGCGGCTCTCCAATGGACTGGCA CGGCTCTCCAGCCCAGTATTGCACCGCCTGCGCCTTCGGGAGGATGAAAATGCTGAACCGGTGGGTACCA CCTACCAGAAGACGGATGCAGCAGTGGAGATGAAGCGGATTAACCGTGAGCAGTTTTGGGAGCAGGCCAA GAAGGAGGAAGAGCTGCGGAAGGAGGAGGAGCGGAAGAAGGCTCTGGACGCCAGGCTCAGGTTTGAACAG GAACGGATGGAGCAGGAGCGGCAGGAGCAGGAAGAACGTGAGCGGCGCTACCGGGAGCGGGAGCAGCAGA TTGAGGAGCACAGGAGGAAACAGCAGAGTCTGGAAGCTGAAGAAGCCAAGAGGAGGTTAAAGGAGCAGTC TATCTTTGGTGACCAGCGGGATGAAGAGGAAGAGTCCCAGATGAAGAAGTCGGAGTCAGAGGTGGAGGAG GCGGCTGCCATCATTGCCCAGCGGCCTGATAACCCACGGGAGTTCTTCAGACAGCAGGAACGAGTGGCAT CGGCCTCTGGTGGCAGCTGTGACGCGCCTGCGCCTGCACCCTTCAACCACCGACCAGGCAGCCACCTGGA CAGCCACCGGAGGATGGCACCCACTCCTATTCCCACCCGGAGCCCATCTGATTCCAGCACAGCCTCTACC CCCATCGCTGAGCAGATCGAGAGGGCCCTGGATGAGGTCACATCCTCGCAGCCTCCACCTCCACCTCCAC CACCTCCACCAACTCAAGAGGCCCAGGAGACTACCCCAAGCCTGGATGAAGAGCTCAGCAAGGAGGCCAA AGTAACAGCAGCTCCTGAGGTCTGGGCTGGCTGTGCGGCAGAGCCCCCTCAGGCACAGGAACCTCCCCTG TTGCAAAGCAGCCCCCTGGAGGACTCGATGTGCACAGAATCTCCAGAGCAGGCTGCCCTGGCTGCCCCTG CGGAGCCTGCTGCCTCTGTCACCTCAGTAGCTGATGTCCATGCAGCTGACACCATTGAGACCACCACTGC CACTACTGACACCACTATTGCCAACAACGTCACCCCTGCCGCTGCCAGCCTCATTGATCTATGGCCTGGC AACGGGGAAGAGGCCTCAACACTTCAGGCTGAACCCAGGGTGCCCACACCACCCTCAGGTGCTGAGGCCT CCCTGGCAGAGGTGCCCCTGCTGAATGAGGCCGCTCAGGAGCCGCTGCCGCCGGTAGGCGAAGGCTGTGC TAACCTTCTTAATTTTGATGAGCTGCCAGAACCTCCAGCCACCTTCTGTGACCCAGAGGAGGAAGTAGGA GAAACGCTGGCTGCCTCCCAGGTCCTAACTATGCCCTCAGCTCTAGAGGAGGTAGATCAGGTGCTGGAGC AGGAGCTGGAGCCAGAACCTCACCTGCTGACCAATGGAGAGACCACTCAAAAGGAGGGGACCCAGGCCAG CGAAGGATACTTCAGTCAGTCACAGGAGGAAGAGTTCGCCCAATCAGAAGAGCCATGTGCAAAGGTTCCG CCTCCTGTATTTTACAACAAGCCTCCAGAAATCGACATCACCTGCTGGGATGCAGACCCAGTTCCTGAAG AGGAAGAGGGCTTCGAGGGTGGTGATTAGTAGCGGCGACTGCCCCCTGGCTGCCCTCGCCAAGGCTGCCT ACCTGCAGTGGCCTCTGGCCAGCCGGCTTGCAGTGCCAGCATTAGCAGCAGCCCCGCCTGGCTCCCACTC TGGATTCCGGCACTGGCCGGGGACCTGTCTGCTTCCTTACCCACAGGGCCTGACTTTTACAGCTTTTCTC TTTTTTTTAAAAGTTGATAGACTTGTACAATTGACTGGTTTTCCTCCCGTTGGTAGTTGAAACAATGTTG CAGATTCCACCCCCTTCCTGCCCAGACCAGATTGTAGCTTAGTTCTTTCTGCTCAGCTGACCAGGGTGGA GGCCTCACCTTGTTTGGGACCTGGTGGGGGTCGGGGGTTCTGGTGGGAAAATGTTCCCCCCATCACTTTT CCTAGTTTTATGTTTCTTGGAAAAAAAATCACTTTGTATTCTCTAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3710497 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710497 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10942 same embryo
	EMAGE:10940 same embryo
	EMAGE:10939 same embryo
	EurExpress:euxassay_006495 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source