EMAGE mouse gene expression spatial database

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EMAGE:11015

Cyc1 cytochrome c-1 ( MGI:1913695)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003443. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003443_01	euxassay_003443_02	euxassay_003443_03	euxassay_003443_04

euxassay_003443_05	euxassay_003443_06	euxassay_003443_07	euxassay_003443_08	euxassay_003443_09

euxassay_003443_10	euxassay_003443_11	euxassay_003443_12	euxassay_003443_13	euxassay_003443_14

euxassay_003443_15	euxassay_003443_16	euxassay_003443_17	euxassay_003443_18	euxassay_003443_19

euxassay_003443_20	euxassay_003443_21	euxassay_003443_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11015_wholemount_strong.wlz

11015_wholemount_moderate.wlz

11015_wholemount_weak.wlz

11015_wholemount_possible.wlz

11015_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11015_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 16 18 19 weak expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6826
Entity Detected:	Cyc1, cytochrome c-1 ( MGI:1913695)
Sequence:	sense strand is shown >T6826 GGCCGCACTAAAGGGGGCCGACATGGCGGCGGCGGCGGCTTCGCTTCGCCGGACGGTACTGGGCCCGCGG GGCGTGGGGCTTCCAGGTGCAAGTGCTCCGGGTCTGCTGGGCGGCGCGCGGTCCCGGCAGCTTCCATTGC GGACACCGCAGGCAGTGTCCTTGTCCTCGAAGTCTGGCCCTTCTCGAGGCCGAAAGGTGATGCTGTCAGC GTTGGGCATGCTGGCCGCGGGGGGTGCAGGGCTAGCTGTGGCCCTTCATTCTGCCGTGAGTGCCAGTGAC CTGGAGCTGCACCCCCCCAGCTACCCATGGTCTCATCGTGGCCTCCTCTCCTCCTTGGACCACACCAGCA TTCGGAGGGGTTTCCAGGTATACAAGCAGGTGTGCTCTTCCTGCCACAGCATGGATTATGTGGCGTACCG CCACCTGGTGGGAGTGTGCTACACGGAGGAAGAAGCCAAGGCGCTGGCTGAGGAGGTGGAGGTCCAGGAT GGCCCTAATGATGATGGGGAGATGTTCATGCGGCCAGGGAAGTTGTCTGACTATTTTCCAAAACCATACC CTAACCCTGAGGCTGCAAGAGCTGCTAACAATGGAGCTTTACCCCCTGACCTCAGCTACATCGTTCGAGC TAGGCATGGTGGTGAGGACTACGTATTTTCCCTGCTCACTGGCTACTGTGAGCCCCCCACTGGGGTGTCA TTGCGAGAAGGCCTCTATTTCAACCCTTACTTTCCCGGCCAGGCCATTGGCATGGCTCCTCCCATCTACA CAGAAGTCTTGGAGTATGATGATGGCACCCCAGCTACCATGTCACAAGTAGCTAAGGATGTCGCCACCTT CCTTCGCTGGGCATCAGAACCAGAGCATGACCATCGAAAACGCATGGGTCTCAAGATGTTGTTGATGATG GGCCTGCTGCTGCCCCTGACCTATGCCATGAAGCGGCATAAGTGGTCAGTCCTGAAGAGTCGAAAGCTGG CTTATCGGCCACCCAAGTGACCCTGTTCAGTATCTGCTTGTCATCTTGCCAGAACAAGCTCTCAGCAGCC CAGGAACAGTCCATGGCCTTCTCAGGCCTGGCCCTTTTCATCAGATACAAGGGCCAGGAGACCAGACTCT TGCTTCTGATCTTCCTTCAGCCCTCAGTGTGGGAATAAATTAAGTTTCTCAACATAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3711323 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3711323 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11013 same embryo
	EMAGE:11017 same embryo
	EMAGE:11014 same embryo
	EMAGE:11016 same embryo
	EurExpress:euxassay_003443 same experiment
	MGI:4824151 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source