EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11021

Nudt6 nudix (nucleoside diphosphate linked moiety X)-type motif 6 ( MGI:2387618)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003447. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003447_01	euxassay_003447_02	euxassay_003447_03	euxassay_003447_04

euxassay_003447_05	euxassay_003447_06	euxassay_003447_07	euxassay_003447_08	euxassay_003447_09

euxassay_003447_10	euxassay_003447_11	euxassay_003447_12	euxassay_003447_13	euxassay_003447_14

euxassay_003447_15	euxassay_003447_16	euxassay_003447_17	euxassay_003447_18	euxassay_003447_19

euxassay_003447_20	euxassay_003447_21	euxassay_003447_22	euxassay_003447_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11021_wholemount_strong.wlz

11021_wholemount_moderate.wlz

11021_wholemount_weak.wlz

11021_wholemount_possible.wlz

11021_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11021_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6246
Entity Detected:	Nudt6, nudix (nucleoside diphosphate linked moiety X)-type motif 6 ( MGI:2387618)
Sequence:	sense strand is shown >T6246 CGGAAAGGCTAGGTGCAGCCATGTGGTGGGTGAGGAGGACCCGCTACTGGTTCTCCACATTGCTTTACGC GGGCGGAGCCAGGCTCCGGCCGGGGCGGCGCACAGCCTCGGGCGGCCTGGAGACCCCGGGCTCCTGCGGG GCCGAGCTACAAGGCGAGCTGGACAGATTTGGGGGCGTCTCGGTGCACTTGTCGCGGCACCATACGTTGC ACGGGCTGGATGCCGCCGCCTTCCGGAGGCTCTTGCAGGCTGCCATTCAGCAGTGGCGCTCAGAAGGACG GATAGCTGCCTGGCTACACATCCCCATCCTGCAGAGCCACTTCATTGCCCCTGCTGCTTCCCTGGGCTTT TGCTTCCACCACGCAAAACCCCATTCCTCAACACTGACTCTGTGGCTGGGAGAAGGACCCAGCAGACTCC CGGGATATGCTACACACCAAGTTGGAGTTGCAGGTGCTGTATTTGATGTTAGCACCAGGAAAGTACTGGT TGTACAAGATCGAAATAAATTGAAAAACATGTGGAAGTTTCCAGGAGGCCTGTCGGAGCCAGGAGAAGAT ATTGACACAGCAGTCCGAGAGGTTTTTGAAGAGACTGGTGTCAAGTCAGAATTCAGGTCCCTGTTGAGCA TCCGGCAGCAACACAGGAGCCCTGGGGCCTTCGGGATGTCAGACATGTACCTGGTCTGCCGCCTGCAGCC TCGTTCCTTCACTATCAACTTCTGCCAGCAGGAATGCTTGAAGTGTGAATGGATTGATCTAGAAAACCTG GCCCGGACTAAACACACGACCCCCATCACCAGCAGGGTGGCTAGGCTACTGCTGTATGGACTCAGGGAAG GGTTTGACAAAATTGACCTCAGCATGGAGGAGCTCCCTGCAGTGTACACAGGCCTGTTTTACAAACTCTA TCACAGGGAGCTGCCGGAGAGTTACAAAGCCGCAACGGGAGCAGACTGAATACCAGATTTCAACCTGACG CCAGTTTTCTCACATATGTTATGTCCATGGTGGAAATTCATAAATGTACATATTTTTAAATCCTCTTTTC AATAAAGGAAATATGTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3500531 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3500531 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11023 same embryo
	EMAGE:11022 same embryo
	EMAGE:11019 same embryo
	EMAGE:11020 same embryo
	EMAGE:11018 same embryo
	EurExpress:euxassay_003447 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source