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EMAGE:11024

Commd3 COMM domain containing 3 ( MGI:88218)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11024 EMAGE:11024 EMAGE:11024 EMAGE:11024 EMAGE:11024
"Pseudo-wholemount" of euxassay_003440. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003440_01 euxassay_003440_02 euxassay_003440_03 euxassay_003440_04
EMAGE:11024 EMAGE:11024 EMAGE:11024 EMAGE:11024 EMAGE:11024
euxassay_003440_05 euxassay_003440_06 euxassay_003440_07 euxassay_003440_08 euxassay_003440_09
EMAGE:11024 EMAGE:11024 EMAGE:11024 EMAGE:11024 EMAGE:11024
euxassay_003440_10 euxassay_003440_11 euxassay_003440_12 euxassay_003440_13 euxassay_003440_14
EMAGE:11024 EMAGE:11024 EMAGE:11024 EMAGE:11024 EMAGE:11024
euxassay_003440_15 euxassay_003440_16 euxassay_003440_17 euxassay_003440_18 euxassay_003440_19
EMAGE:11024 EMAGE:11024
euxassay_003440_20 euxassay_003440_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11024Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11024_wholemount_strong.wlz
11024_wholemount_moderate.wlz
11024_wholemount_weak.wlz
11024_wholemount_possible.wlz
11024_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11024_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 15 16 17 18
pancreas
moderate moderate
regionalmoderate expression: see section 08 09 10 11 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5929
Entity Detected:Commd3, COMM domain containing 3 ( MGI:88218)
Sequence:sense strand is shown

>T5929
GCGGCGCGCTCACAATGGAGCTCTCGGAGTCTGTGCAAAGAGGCATCCAGACGCTGGCGGATCCCGGTTC
CTTTGACTCCAACGCCTTCGCGCTTCTCCTCCGGGCGGCTTTCCAGAGCCTGCTGGACGCCCGGGCGGAC
GAGGCCGCGCTAGATCACCCATACTTGAAACAGATCGACCCAGTGGTTTTAAAGCATTGTCATGCAGCAG
CTGCAACTTGCATCCTGGAGGCAGGAAAGCACCAAGTTGACAAGTCTACTCTAAGCACTTATTTAGAAGA
CTGTAAATTTGACCGAGAGCGAATAGAACTGTTTTGCACCGAGTATCAGAACAATAAGAATTCTCTAGAA
ACCCTACTGGGAAGTATAGGCAGATCTCTCCCTCATATAACTGATGTTTCTTGGCGCTTGGAGTACCAGA
TAAAGACCAACCAACTTCATAAGATGTACCGACCTGGATATTTGGTGACCTTAAATGTAGAGAACAATGA
TTCCCAATCCTACCCAGAGATTAATTTTAGTTGCAACATGGAACAGTTACAGGACTTGGTGGGGAAACTT
AAGGATGCTTCAAAAAGCCTGGAAAGAGCAACTCAGTTGTAATTTGGGGAGGCTGATGACCCCAGGAGCC
TAGAGGAAAACCAGACGCGCCTTGTCTCCTGCTGGACCATCGTTCGCGCGAGCTGGATGTCATTTTCAGT
GGAAAAAGAGCTAGCACCCTTTTGAAGTGATGCCAACCATCAATTTTGACACTTTTTTTACACATGTGAA
ATGGTTAAGAGGGAGACTCTTAAGCATCTGGATTTTCAGTATTACTCTTGCGTTCCTTCTATCTTGAGCG
TCTTCAAGCCCTATTAAAGTATCGTACCCTGAATAAATACGGTGGTATTCCCACAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3482368 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3482368 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11025 same embryo
 EMAGE:11026 same embryo
 EurExpress:euxassay_003440 same experiment
 MGI:4823999 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS