EMAGE mouse gene expression spatial database

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EMAGE:11084

Fbxo30 F-box protein 30 ( MGI:1919115)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003659. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003659_01	euxassay_003659_02	euxassay_003659_03	euxassay_003659_04

euxassay_003659_05	euxassay_003659_06	euxassay_003659_07	euxassay_003659_08	euxassay_003659_09

euxassay_003659_10	euxassay_003659_11	euxassay_003659_12	euxassay_003659_13	euxassay_003659_14

euxassay_003659_15	euxassay_003659_16	euxassay_003659_17	euxassay_003659_18	euxassay_003659_19

euxassay_003659_20	euxassay_003659_21	euxassay_003659_22	euxassay_003659_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11084_wholemount_strong.wlz

11084_wholemount_moderate.wlz

11084_wholemount_weak.wlz

11084_wholemount_possible.wlz

11084_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11084_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6668
Entity Detected:	Fbxo30, F-box protein 30 ( MGI:1919115)
Sequence:	sense strand is shown >T6668 CCACGCGTCCGCCCACGCGTCCGTGTGTGGAAGCTGATGGTACTTCAGAACCTTCCAGTTCACTTGTAGT ACCAGAACAACTGAGGGAAATAAGTCCTTTCAGTGCTTTGCCAGATAGTACATTTCAGCAAATCCTCATG CCAGATGAAGACGATGAGAAGGACTTGTGTTGGAAAAAAGTAGACTTGGGAGACTTGAAGGATGTTAATG GTTCACCTTTCAGTCATGCGCCTTCATTCAAGTTTCTTTCTAATTCATGGTATATACCAAAGGAAGACAA AGCAGTTGATACATCAGATTTAGAAGTTGCAGAAGATCCCATGGGCCTCCAAGGAATAGATCTGATTACA GCAGCATTACTGTTTTGTCTGGGAGATTCTCCAGGTGGGAGAGGTATATCTGATAGCCGCATGACTGATG TTTATCACGTTGACTTTGGGACACAGACTTTTTCACTTCCATCTGCAATATTAGCTACAAATACAATGGT TGGAGAAATAGCTTCGGCTTCAGCTTGTGATCATGCCAATCCACAGCTTTCAAATCCAAGCCCTTTTCAG ACACTTGGGCTGGACTTAGTTTTGGAATGTGTTGCTAGGTACCAGCCTAAGCAGCGTTCAATGTTTACCT TTGTATGCGGACAGCTATTTAGAAGAAAAGAATTTTCTTCCCATTTTAAGAATGTGCATGGTGACATTCA TGCTGGACTCAATGGATGGATGGAACAGAGGTGTCCTTTAGCTTATTATGGTTGTACCTATTCTCAGCGT AGATTTTGCCCATCGACACAAGGAGCAAAGATCATACATGATCGCCATTTAAGGTCATTTGGAGTTCAGC CATGTGTATCTACAGTATTAGAAGAACCTTCTAGAAACTGTGTGTTGGGATTGCGTAGTGACCATCTAAG TAGTCTCCCTTTTGAGGTACTCCAACATATTGCAGGCTTCCTTGACGGCTTCAGTTTATGCCAGCTTGCA TGTGTATCCCGGCTAATGAGGGACGTTTGTGGCAGTTTACTTCAGTCTCGTGGAATGGTAATCTTGCAGT GGGGGAAGAAGAAGTATCCGGAAGGAAATTCATCATGGCAGATAAAAGAGAAGGTATGGCGATTCAGTAC TGCATTTTGTTCTGTTAATGACTGGAAATTTGCTGACATCCTGAGCATGGCTGACCACTTGAAGAACTGC AGTTACAACGTGATAGAGAAACGGGAGGAAGCCATCCCATTGCCGTGTATGTGTGTGACTCGAGAGCTTA CAAAAGAAGGACGTTCCCTGCGCTCAGTTTTAAAGCCTGTGCTTTAAGGCTGTAACTTCTTCACTTGCAC ACACATGCAAGCACCTGGTATCAGTTCTTTGTATGATGTGATACTTTATTAATGCATTAAAGACTACAAC TAGCCAAATTGATTGTCACTATGTATATACTGTTTTGCAGTGACATATATTTTTATAATGTACTATTAGT TAGAAAAATGTTGCCTTAATATTTGAAATGTGTGAAATTGTTGGGACATGCTAGACAGGGTGATCTTTTC TGCATAATGTTAGTATTAATGGTGTGCACATTTAAATTCTGAAATCTTTGCTCCTCAAACTAAAACGATC TTGACCAAAACAATTCTTTATATTTTCTATGGATTGAAGCCCATGTTATCAAAAAGGAAAACTTTGATTC GAAATTTTTGTTGCATAGATTTTTCACATAATACATTTGATGTTGAAACACTGCCATAAGATTATAATCA AGTTTTCCTGGTACTTTCATAATTATTGATCACAAGTGTAATACAGTATTCACATAAGGCACAATAGTGA CTTCATGTCTTTAAAACAACATACAAAGATATTTTATCTTCTGTTCTTAAAACTCTTATGCAAAAAATGG TCATATGCAGGTCTTTTTTTTTCGTTAAATACCGTATCTGAAGAGTTGTGGCACAATGTATTATATGACA GATTAAAGTGAAAACAAAGGAAGCCTGTGAATTTAACTTGAAAATTTTCATGGTATTTCATATTTTCTAG GTATCCAATGTTTGAAAAGAAAATATACCTCACTAAAAGTTTGAGTTTGGAATATTGTTCGAATAAACTT CAAATATTCAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601336 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601336 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11088 same embryo
	EMAGE:11087 same embryo
	EMAGE:11089 same embryo
	EMAGE:11086 same embryo
	EMAGE:11085 same embryo
	EurExpress:euxassay_003659 same experiment
	MGI:4824804 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source