EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11108

Acot13 acyl-CoA thioesterase 13 ( MGI:1914084)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001075. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001075_01	euxassay_001075_02	euxassay_001075_03	euxassay_001075_04

euxassay_001075_05	euxassay_001075_06	euxassay_001075_07	euxassay_001075_08	euxassay_001075_09

euxassay_001075_10	euxassay_001075_11	euxassay_001075_12	euxassay_001075_13	euxassay_001075_14

euxassay_001075_15	euxassay_001075_16	euxassay_001075_17	euxassay_001075_18	euxassay_001075_19

euxassay_001075_20	euxassay_001075_21	euxassay_001075_22	euxassay_001075_23	euxassay_001075_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11108_wholemount_strong.wlz

11108_wholemount_moderate.wlz

11108_wholemount_weak.wlz

11108_wholemount_possible.wlz

11108_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11108_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

weak

regional

weak expression: see section 15 16 17

midgut

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17

chondrocranium

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1120
Entity Detected:	Acot13, acyl-CoA thioesterase 13 ( MGI:1914084)
Sequence:	sense strand is shown >T1120 CCTCNANNCTGTTGGCCTACTGGCTAGGACCAGGGCGACCGGGGCGCGCGATCCTTTCTCCCGAGCACGA CGCGACAGCGGCCGGGCTTGCAGACTTGACCTTCCACACCCTTGTCCTCTCACAAACGTCTCTTCCAGAG TTCATTCTCGCAGAGCCCAGACTCTTGCTTTGCGTCCACGATGAGCAGCATGACCCAGAACCTACGAGAA GTAATGAAGGTTATGTTCAAAGTTCCCGGTTTTGATAGAGTTTTGGAAAAGGTGACGCTTGTCTCGGCTG CTCCTGAGAAACTAATCTGTGAGATGAAGGTGGAAGAGCAGCATACTAATAAACTGGGCACGCTCCATGG AGGCTTGACAGCAACCTTAGTGGACAGCATCTCGACCATGGCTCTAATGTGCACAGAAAGAGGAGCACCC GGAGTCAGTGTGGACATGAACATAACGTACATGTCACCTGCTAAGATAGGAGAAGAAATAGTGATCACAG CACACATTCTGAAGCAAGGAAAGACACTTGCATTTGCCTCAGTGGATCTGACCAACAAGACCACAGGAAA ATTAATAGCACAAGGCAG
Notes:	The probe template was PCR amplified from IMAGE:2135423 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2135423 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11110 same embryo
	EMAGE:11111 same embryo
	EMAGE:11109 same embryo
	EMAGE:11107 same embryo
	EurExpress:euxassay_001075 same experiment
	MGI:4822925 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source