EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11116

Rab30 RAB30, member RAS oncogene family ( MGI:1923235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001143. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001143_01	euxassay_001143_02	euxassay_001143_03	euxassay_001143_04

euxassay_001143_05	euxassay_001143_06	euxassay_001143_07	euxassay_001143_08	euxassay_001143_09

euxassay_001143_10	euxassay_001143_11	euxassay_001143_12	euxassay_001143_13	euxassay_001143_14

euxassay_001143_15	euxassay_001143_16	euxassay_001143_17	euxassay_001143_18	euxassay_001143_19

euxassay_001143_20	euxassay_001143_21	euxassay_001143_22	euxassay_001143_23	euxassay_001143_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11116_wholemount_strong.wlz

11116_wholemount_moderate.wlz

11116_wholemount_weak.wlz

11116_wholemount_possible.wlz

11116_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11116_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate

moderate

regional

moderate expression: see section 09 10 11 12 15 17 18 19

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 13 15

metencephalon basal plate

moderate

regional

moderate expression: see section 09 10 12 15 17 19 weak expression: see section 11 18

facial vii ganglion

weak

regional

weak expression: see section 05 06 21 22 23

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 20

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vagus x ganglion

weak

regional

weak expression: see section 09 19

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 09 19 20

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 15 18 weak expression: see section 14 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4265
Entity Detected:	Rab30, RAB30, member RAS oncogene family ( MGI:1923235)
Sequence:	sense strand is shown >T4265 GCAGGACCAGGGCAGGGACTGACTCCAGTCCTTGGACTTAGGACACCGCAGCACCTTCTCTTGCGTGCGT GACTCATTCGTATATCATTTCTTTTTATGCCTGGGGTGGGGAGAAAAATCCCAAACCAGCTGTGTAAATG AGTATGGAAGATTATGATTTCCTGTTCAAAATTGTTTTAATTGGCAACGCTGGAGTGGGAAAGACGTGCC TAGTCCGAAGATTCACTCAGGGTCTTTTCCCCCCAGGTCAAGGAGCCACAATTGGAGTTGATTTTATGAT TAAGACAGTGGAGATTAATGGTGAAAAAGTGAAGTTACAGATCTGGGACACCGCAGGTCAAGAGAGATTT CGCTCCATCACTCAAAGTTACTATCGAAGCGCCAATGCCTTGATCCTTACCTATGACATCACCTGTGAGG AATCCTTCCGCTGCCTTCCTGAGTGGTTGCGGGAGATAGAACAGTATGCTAGCAATAAAGTCATCACTGT GTTAGTAGGCAACAAGATTGACCTGGCTGAAAGGCGAGAGGTCTCCCAGCAGAGAGCAGAAGAGTTCTCA GAGGCTCAGGACATGTATTACCTGGAGACTTCAGCCAAGGAATCCGACAATGTGGAGAAACTCTTCCTTG ACTTAGCATGCCGACTCATCAGCGAAGCAAGACAGAACACACTGGTGAACAATGTATCGTCACCCTTACC CGGAGAGGGGAAAAGCATCAGCTATTTGACTTGTTGTAATTTCAACTAAAGGCTGAGGCAAAGAGAATCA AAGGGAATCAGTAGTTGCCTTGGTGGGCCGTACGTTGCTAGGGAATCTGGCAATGACTATGGCTCCCGCT CTTGGACCTTCTGACTCCTGTAGGCTCCAGAGCTTACCAAGCATGCAGGCCAAGGGCCTTGACTGCAGGC CAGCATTAGCAGAACACATAATGGTTTCACCCTTTTGCAGTCTGGCGTTGGAGCAAGGAGAAAATTGCAC TAAGCGCTCCATGATCTGCAGAGCATGTTGCTTTTGTTTTTAAAAAAGCAAGTAAAAAATGCATTCCTGA ACACAGAGCAGGGGATCGTGTACTGTAGGAATCCTTCTGTGTGTCAGCGGGTGCATGAGGGGCTATTCTT TAGGCTTCAGTGGTAATCTGGTGCCCATGGATTTCTTGACTACCAGGTAAACCAAAACTGGAAAGGCCAA GTACTGTCTCTGTAGTACTTATTGAGGACCCCATGGAGATTTTGAAAAGTGTTATTTCTTTTGTCCACAA GCACTTTCAAAACCTTTGGGATATAAAAATGGGAACTCTTCTCACGACCAACAGTAAAAATTATTGTTTA ACAATATATACAAGCTCCATGACTCTTTTTGGTGCTAATGATTATGCTATTTCACAGACCAAACGTTTTA GTACATTGATACCCTTAGATGTATTACCTAAAAATAAAAAGAGAATGGGGGAAATCCATGAGTCAGCAGT CTTCTTCAGTCTCATTACCACCATTTCTAGGATGGGTTTGGATATGAGAAGCCCTTCTTCTTTCATATTC TCCTTGAAATTTATTAACATTCTTCTATTCTTTCACACTTATCGTGCCCTTTAGGTAAATCAAAGTATTA AAAAAATGGGGAAAAATATAAATTATGACATTCTAATAAAGAAGATTAGTTGTAGGACATGTTGAGTAGC ATGAGAAAACAGAGCAGATATGACCAAAATACTGTGGACTATGGACTGACTGCAGGGCAGACTTCCAGAC AGAGTGCAGCTCTCTAACAGGTCACGCCTTTTCCTTTCGGGAACTAGCCTTGGAGAGGCCAAGGTTTAGG ATTTTGTAGACATTTTTAGTATAATAAGTGAGAATAGCAAGAGATGAAAAGTCTCACAAATCATTTTTGT ATCTTCAATAAACTTAGGAAAGTGTGCTCAGGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503909 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503909 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11113 same embryo
	EMAGE:11114 same embryo
	EMAGE:11117 same embryo
	EMAGE:11112 same embryo
	EMAGE:11115 same embryo
	EurExpress:euxassay_001143 same experiment
	MGI:4827574 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source