EMAGE mouse gene expression spatial database

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EMAGE:11126

Lrrc46 leucine rich repeat containing 46 ( MGI:1916547)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001006. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001006_01	euxassay_001006_02	euxassay_001006_03	euxassay_001006_04

euxassay_001006_05	euxassay_001006_06	euxassay_001006_07	euxassay_001006_08	euxassay_001006_09

euxassay_001006_10	euxassay_001006_11	euxassay_001006_12	euxassay_001006_13	euxassay_001006_14

euxassay_001006_15	euxassay_001006_16	euxassay_001006_17	euxassay_001006_18	euxassay_001006_19

euxassay_001006_20	euxassay_001006_21	euxassay_001006_22	euxassay_001006_23	euxassay_001006_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11126_wholemount_strong.wlz

11126_wholemount_moderate.wlz

11126_wholemount_weak.wlz

11126_wholemount_possible.wlz

11126_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11126_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 10 11 14 15 16 17 18 19 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 11 12 13 16 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4252
Entity Detected:	Lrrc46, leucine rich repeat containing 46 ( MGI:1916547)
Sequence:	sense strand is shown >T4252 GCGAGACCTCAAGGAACGGTTCTTTCTATTCCTTCTGGTCCTTTCAGTTTAAGGCCAGTAAAGCCACGCT ACACACCTCTAACTCCAGATCCTTCGTTCCCATTTACTCCTATTTTCCACCGCACCCCCAATAATTCTCA GAACCCCCTGCCCTTGTCTCCTCAGTCTTCCCTCCAACCTGGGTTCAACCAGCCCAACCGTCTAAGCTTC TCTGCTCGCCTTCGTCCATCTACCTGCCTCAAGCAGCCGCTGTTTATTTTAGATCATGCCAGGAGACGAG CAGGAAGCTAAGAAAGCTGCCCAGAGAACCGAGGAAGGTGTCCACATTACTGAAGCCCTTATCACCAAGC GGAACCTGACCTTCCCTGGAGATGAAGACCTGTCAGAAAAGATGTTTCACACTCTCGGTGAACTGGAGAC TGTCCGCCTGGACGGGGAAGGGATTACTTGTATCGGGAATTTAGAGAAACTCCGGAATATTCACAGCCTC TACCTGCAATCGAATAAGATCCAGCGCATTGAGAACTTGGCATGCATCACCTCCCTGCGCTTCCTGTCTT TGGCAAGAAACCAAATCAGGCATGTGGAAAACCTCCTTGACCTCCAGTACCTCCAGTTTCTGGACCTGTC TGAGAACCTGATAGAAACGCTGAAGCTAGATGAGCTCCCTGAGAGCCTTCTCATCCTCAACCTGTGTGGA AATCCCTGCACCAACCAGGAAGGCTACAGGAAGATGGTGATAGGAGCCCTGCCACTGCTCTTGGACCTGG ACAAGCAGCCTATCCTGGAGCGGTGGACCTCAGATGAGGAGGATAAATCCTCAGATGATGATGATGAGTT TCCAGAGCTCAATGGCCCCTTCTGCGCAGAGCGAGGATTCTTCAAGGACTTGGAGCAGGAACTGCATCAG CATCAGGAACGCAGGCAGCAGGCAGCACTGACAGAGCACTTGTCAAGGATGGAGACACAGCCTGTCCTCA CAGACCTGCCCCTGCTGCCTGCTGTGCCCATGGCTGGGGACTGCAGCTCTACTGCCACTGACCAGCCTGG GAAGGAGTCAGCCCCTAAAGCTACCTCCTCAACTCAGACTGCCTCTACTACCAAGAAACAGGTTTCCAAG AACCAGAAAAGTTCTGTCCAGGCAAGGAAGGGGGCTCTAGCAGCCACAACCTCCAAGACCTCTCAGGCTG CAACCCCCAGCATGACAAAAATGACAAACAAGAAAAGCACAAAGTAGCGCACAGCCTCTATGCACGCGCC ACCTCCTCAGGCTTTGCCCCAATAAAGTGTCTCTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503387 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503387 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11124 same embryo
	EMAGE:11123 same embryo
	EMAGE:11125 same embryo
	EMAGE:11122 same embryo
	EMAGE:11127 same embryo
	EurExpress:euxassay_001006 same experiment
	MGI:4825996 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source