EMAGE mouse gene expression spatial database

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EMAGE:11156

Lcp2 lymphocyte cytosolic protein 2 ( MGI:1321402)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003585. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003585_01	euxassay_003585_02	euxassay_003585_03	euxassay_003585_04

euxassay_003585_05	euxassay_003585_06	euxassay_003585_07	euxassay_003585_08	euxassay_003585_09

euxassay_003585_10	euxassay_003585_11	euxassay_003585_12	euxassay_003585_13	euxassay_003585_14

euxassay_003585_15	euxassay_003585_16	euxassay_003585_17	euxassay_003585_18	euxassay_003585_19

euxassay_003585_20	euxassay_003585_21	euxassay_003585_22	euxassay_003585_23	euxassay_003585_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11156_wholemount_strong.wlz

11156_wholemount_moderate.wlz

11156_wholemount_weak.wlz

11156_wholemount_possible.wlz

11156_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11156_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6701
Entity Detected:	Lcp2, lymphocyte cytosolic protein 2 ( MGI:1321402)
Sequence:	sense strand is shown >T6701 GCAGAAAAGAGTCCTCTTCAACAAGGGTGTTCCTGAGAGCATCTGGGAATCAGAAGAGCCAAGGACACCT CAGAGCTCTGGATCTGGGCTTTTCCCCTGTTCATGGCTGCATCGCCTCTCCTCAGTTTGTGAGCCTCTCC CTGTAGAGCAGCCATGGCCTTGAAGAATGTCCCGTTTCGCTCAGAGGTCCTAGCCTGGAACTCAGACAAC CTCGCTGACTATTTCAGGAAGCTGAACTACAGGGACTGTGAGAAGGCTGTGAAGAAATACCACATTGATG GTGCTCGGTTTTTGAACCTGACAGAAAATGACATTCAGAAGTTCCCTAAGCTGAGGATGCCACTTCTCAG TAAGTTGAGTCAAGATATCAACAAGAACGAAGAGAGAAGGAGCATTTTCACACGCAAACCCCAGATTCCA CGGTTTCTTGAGGAGACAGAAAGCCACGAAGAAGACGATGGTGGCTGGTCATCCTTTGAAGATGACTATG AAAGTCCCAATGATGATGATCCAGATGGGGAGGACGATGGTGACTATGAGTCCCCTAATGAGGAGGAACA GGCCCTGGTGGACGATGCTGCTGACTATGAGCCTCCACCCTCCAACAACGAGGAGGCTCTGCAGAGCTCC ATCCTGCCCCCCAACTCTTTCCACAACACCAACTCCATGTACATCGACCGACCTCCCACTGGCAAGGTCT CCCAGCAGCCTCCAGTGCCCCCACTGAGACCAAAACCTGCCCTCCCTCCCCTGCCAACAGGCAGGAATCA CTCGCCACTGTCTCCACCCCATCCCAACCACGAAGAGCCCAGCAGAAGTGGAAACAACAAGACAGCAAAG TTGCCTGCTCCATCAATAGACAGAAGCACAAAACCTCCCCTGGACCGCTCACTGGCCCCTCTTGACCGAG AGCCCTTCATACTAGGAAAGAAACCACCATTTTCTGATAAGCCCTCAGCCCCATTGGGGAGGGAACATTT ACCCAAGATACAAAAGCCTCCTTTGCCACCAGCCATGGACAGACACGAAAGAAATGAAAGACTCGGACCC GTGACAACGAGGAAGCCACCAGTTCCAAGGCATGGAAGGGGACCAGACAGAAGAGAGAATGATGAAGATG ATGTGCATCAGAGACCTTTGCCACAGCCGTCACTACCTTCCATGAGCTCCAACACATTCCCTTCGAGATC TGTCCAGCCCAGCTCCAAGAACACATTCCCATTGGCCCACATGCCGGGAGCCTTCTCAGAAAGCAACATT GGCTTTCAGCAGAGTGCCTCCCTGCCGCCATACTTCTCTCAAGGTCCAGGCAACAGGCCACCTCTCAGAA GTGAAGGCAGAAACTTACCCTTGCCGGTTCCAAACAGGCCTCAGCCTCCATCTCCTGGGGAAGAAGAGAC ACCCTTAGATGAAGAGTGGTATGTCTCTTATATCACCCGACCAGAGGCAGAAGCTGCTCTGAGGAAGATA AACCAGGATGGCACCTTCCTGGTTAGAGACAGCTCTAAGAAGACAGCGAACAATCCGTATGTCCTCATGG TGCTGTACAAAGACAAAGTTTACAACATCCAAATCCGCTACCAGGAGGAAAGTCAAGTCTACTTGTTGGG CACTGGACTTAGAGGAAAAGAGGACTTCCTGTCTGTATCAGATATTATTGACTACTTCAGGAAAATGCCG CTGTTGCTCATTGATGGAAAGAATCGAGGGTCCAGATACCAGTGCACACTAACACACGCTGCAGGCTGTC TGTAGCAAGTTAGCCAAGAAATGAGCCTTTCTGACTTGGTGGTCACTGCGGCGAAATCACGTGACCTTAG CAAATCGCCGAGCACTTAGGAGTAGACTGTTAACTCGGGCCCAGAGTTCTGCCCTGCCCTTTTAAAGTAT CTGAAGATTGTTGAATACCACATGATTTATTTATTTTCTTTGATGTTTATAGAGTATAGAAGTGATCATG TTCACATTTGAAGTCTAAGAGTGCATGGCAGACATGAGATTTTGAACATCCATCCCAAAATACAGTACTT GATACAGTACAGAAATAATGTGGAGCAAGCTGAAAAAGTACTGAAACTGGTCATTTTTGATATATTTTAT AATGAATCTTTTGTCAGGCCGAGGCATGTTCTGTTTTTTACACAACTTAAAATGATTAAAATTCATTTTG GAGCAGCAATAACTTAAGAGGCTCCAAAAAAAAAAAAAATAGAACTCCATCGTTTCTTATTTGGTTTTAC AGGGTGTGTTTTTATGTGTTCCCTTTCGTAATGAGATTAATGCATGTGGGGATAACTTCCTGGGATTAAA GTTATCGTTTGCTTTACTTTTTTCGATCCTTAAGACAACGTTGTTTGGTCCTTGCTGTTATATTTTTATT TTAAAGCAAATGTAAGCTACCTTTCTGAACATACAAGAAGAAAATAGGCTTAGGTTCAGTGATGCAGCTT TGCTGTCAAACATGCTGGGCTTCTTGTTGCTTCTCTTATCACAGACCCTCATGTCTCCATCCATGGTGGC CTCCGTTCTAGTACTGAAGGTCTCCTGGGAAGACGAGCAATGCTTGGTAAACACACACACACGCACACAC ACACACACACACACACACACACACACACACACACACTTCGAGCCTACCTTCCTACTAGATGATACTTGCA TTCGCTGGCCTTCAGTTAGACAGTTTGACTTTCTGGGATGGTCGCCTGTTCCTGTTCCTTTGGGCCTGCA ACAGTTCATAGAGGATCATGAAGTGAGAATGTGAGGACATCCGTTCACTTCATGGTGACTGGGAAACAAA AGAAAGGACAAGAAGCACATTTCCCTCCATGTCTTGCATCTTCTGCCTAACTGGGCTTCATCTCCTAAGA TTCCACCACTGCCCAATGCTGCCCTGGTCGGGGGCAGGGGGCCAAGCTTCTACCATGTGGGCCTTTGGAG AATTCTATCCAAACCTCTGCTCTGGTTTTCTGCTTTATTAGCCCAACAAGTATGTTGCAAAACTGCTATC TTGTCCTCAGATTGGCTTTTAAACCCTTTGAAGCACCTCAGATCTCCTTCAGTTTTGAAATCACACTTAA CAAAAGGTAGAGAGAGATTTCTGGCCTATGTTGGTTTAACTTGCCATTTTTGACTTTATGATGGCATGGA AGTGATGCTTGTTAAGCACGAACTGTACGTCAAAGTTTGAATTTTGCTTTTCTGGGGCTAGCAGCAGATG CTATGAAATGTAAGAGTCTTGGCTAGCAGGTGATCACCAGGGCACCCATCAGTACTTTACATGTTTTATG TTGAAGCTGATTTTTTTCTTTTTGTGGAGGGGCTGCTATGAACGCTCAATAAATTAAATAATGCATTCAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3603181 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3603181 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11157 same embryo
	EMAGE:11153 same embryo
	EMAGE:11152 same embryo
	EMAGE:11155 same embryo
	EMAGE:11154 same embryo
	EurExpress:euxassay_003585 same experiment
	MGI:4825889 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source