EMAGE mouse gene expression spatial database

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EMAGE:11159

Sec11c SEC11 homolog C (S. cerevisiae) ( MGI:1913536)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003588. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003588_01	euxassay_003588_02	euxassay_003588_03	euxassay_003588_04

euxassay_003588_05	euxassay_003588_06	euxassay_003588_07	euxassay_003588_08	euxassay_003588_09

euxassay_003588_10	euxassay_003588_11	euxassay_003588_12	euxassay_003588_13	euxassay_003588_14

euxassay_003588_15	euxassay_003588_16	euxassay_003588_17	euxassay_003588_18	euxassay_003588_19

euxassay_003588_20	euxassay_003588_21	euxassay_003588_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11159_wholemount_strong.wlz

11159_wholemount_moderate.wlz

11159_wholemount_weak.wlz

11159_wholemount_possible.wlz

11159_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11159_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 17 18

trachea

weak

regional

weak expression: see section 12 13

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 21 22 weak expression: see section 20

clavicle

moderate

homogeneous

moderate expression: see section 08 09 10 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6724
Entity Detected:	Sec11c, SEC11 homolog C (S. cerevisiae) ( MGI:1913536)
Sequence:	sense strand is shown >T6724 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCGGACGCGTGGGCAGACAGAGCCGGCCGGCCGCCT GGGCCCCCAGAGACCCCGCCATGGTTCGTGCGGGCGCCGTGGGGACGCATCTCCCCACGTCCAGCCTGGA CATCTTTGGGGACCTGAGGAAGATGAACAAACGACAGCTCTACTACCAGGTTTTAAACTTTGCCATGATC GTGTCTTCTGCGCTCATGATCTGGAAAGGCCTGATTGTTCTCACGGGCAGCGAGAGTCCCATCGTGGTGG TACTCAGTGGCAGTATGGAGCCGGCCTTCCACAGAGGAGATCTGCTGTTCCTCACGAATTTCCGGGAGGA CCCCATCAGAGCTGGTGAAATAGTTGTTTTTAAGGTTGAAGGAAGAGACATTCCGATAGTTCACAGAGTA ATCAAGGTTCATGAAAAAGATAATGGTGACATCAAGTTTCTGACTAAAGGAGATAATAATGAAGTCGATG ATAGAGGCTTGTACAAAGAAGGCCAGAACTGGCTGGAGAAGAAGGACGTGGTAGGAAGAGCTCGAGGGTT CTTACCTTATGTTGGCATGGTCACCATCATAATGAATGACTATCCAAAATTCAAGTATGCTCTTTTGGCT GTGATGGGTGCATATGTGTTACTAAAACGTGAATCCTAACATGAGAAGCAGTTCCTGGGACCAGACCGAA ATGAGTTCTGTTGAAAAAGAGAAAAGCTAATATATTTGAAATGTTCCACTTTCTGTATAAAGAGAAACCA GGTGGGGATGTTTTTGTCTTATCTAAATAAATGATTCACCAGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3666130 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3666130 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11163 same embryo
	EMAGE:11161 same embryo
	EMAGE:11158 same embryo
	EMAGE:11160 same embryo
	EMAGE:11162 same embryo
	EurExpress:euxassay_003588 same experiment
	MGI:4827930 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source