EMAGE mouse gene expression spatial database

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EMAGE:11176

Dusp3 dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related) ( MGI:1919599)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001212. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001212_01	euxassay_001212_02	euxassay_001212_03	euxassay_001212_04

euxassay_001212_05	euxassay_001212_06	euxassay_001212_07	euxassay_001212_08	euxassay_001212_09

euxassay_001212_10	euxassay_001212_11	euxassay_001212_12	euxassay_001212_13	euxassay_001212_14

euxassay_001212_15	euxassay_001212_16	euxassay_001212_17	euxassay_001212_18	euxassay_001212_19

euxassay_001212_20	euxassay_001212_21	euxassay_001212_22	euxassay_001212_23	euxassay_001212_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11176_wholemount_strong.wlz

11176_wholemount_moderate.wlz

11176_wholemount_weak.wlz

11176_wholemount_possible.wlz

11176_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11176_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 09 10 19 20

not examined

regional

not examined expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4202
Entity Detected:	Dusp3, dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related) ( MGI:1919599)
Sequence:	sense strand is shown >T4202 CCGCTGACCCGGCTTCTCCCCCTCGTTCGCTCCTGCCCTGGCGTGCAGCGCCCCGCCGCCGCCATGTCCA GCTCGTTCGAACTCTCGGTGCAAGATCTCAACGACCTGCTCTCGGATGGCAGCGGCTGCTACAGCCTGCC GAGCCAGCCCTGCAACGAGGTCGTCCCGAGGGTCTACGTGGGCAACGCGTCTGTGGCTCAGGACATCACC CAGCTGCAGAAACTGGGCATCACCCACGTCCTGAATGCTGCCGAGGGCAGGTCCTTCATGCACGTCAACA CCAGTGCTAGCTTCTACGAGGATTCTGGCATCACCTACTTGGGCATCAAGGCCAATGATACGCAGGAGTT CAACCTCAGTGCTTACTTTGAAAGGGCCACAGATTTCATTGACCAGGCGCTGGCCCATAAAAATGGCCGG GTGCTTGTCCATTGCCGCGAGGGCTACAGCCGCTCCCCAACGCTAGTTATCGCCTACCTCATGATGCGGC AGAAGATGGACGTCAAGTCTGCTCTGAGTACTGTGAGGCAGAATCGTGAGATCGGCCCCAACGATGGCTT CCTGGCCCAACTCTGCCAGCTCAATGACAGACTAGCCAAGGAGGGCAAGGTGAAACTCTAGGGTGCCCAC AGCCTCTTTTGCAGAGGTCTGACTGGGAGGGCCCTGGCAGCCATGTTTAGGAAACACAGTATACCCACTC CCTGCACCACCAGACACGTGCCCACATCTGTCCCACTCTGGTCCTCGGGGGCCACTCCACCCTTAGGGAG CACATGAAGAAGCTCCCTAAGAAGTTCTGCTGCTTAGCCATCCTTTCCTGTAATTTATGTCTCTCCCTGA GGTGAGGTTCAGGTTTATGTCCCTGTCTGTGGCATAGATACATCTCAGTGACCCAGGGTGGGAGGGCTAT CAGGGTGCATGGCCCGGGACACGGGCACTCTTCATGACCCCTCCCCCACCTGGGTTCTTCCTGTGTGGTC CAGAACCACGAGCCTGGTAAAGGAACTATGCAAACACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4489142 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4489142 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11180 same embryo
	EMAGE:11179 same embryo
	EMAGE:11177 same embryo
	EMAGE:11178 same embryo
	EurExpress:euxassay_001212 same experiment
	MGI:4824417 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source