EMAGE mouse gene expression spatial database

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EMAGE:11207

Coro1a coronin, actin binding protein 1A ( MGI:1345961)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003404. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003404_01	euxassay_003404_02	euxassay_003404_03	euxassay_003404_04

euxassay_003404_05	euxassay_003404_06	euxassay_003404_07	euxassay_003404_08	euxassay_003404_09

euxassay_003404_10	euxassay_003404_11	euxassay_003404_12	euxassay_003404_13	euxassay_003404_14

euxassay_003404_15	euxassay_003404_16	euxassay_003404_17	euxassay_003404_18	euxassay_003404_19

euxassay_003404_20	euxassay_003404_21	euxassay_003404_22	euxassay_003404_23	euxassay_003404_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11207_wholemount_strong.wlz

11207_wholemount_moderate.wlz

11207_wholemount_weak.wlz

11207_wholemount_possible.wlz

11207_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11207_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 12 13 14 15 16 17

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 17 18 19

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

not examined

regional

not examined expression: see section 08 09 10 11 12 13 14 15 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5953
Entity Detected:	Coro1a, coronin, actin binding protein 1A ( MGI:1345961)
Sequence:	sense strand is shown >T5953 CCCACGCGTCCGCGGTGGCCAGCCTGCAGCTCCTCCATCCTTCTCTCCTTCCTCCTCTACTTGGGAGGGG TCACGCTCAGCTCTCCGCAGCAGGATGAGCCGGCAGGTGGTTCGCTCCAGCAAATTCCGCCACGTGTTTG GACAGCCAGCCAAGGCTGACCAGTGCTATGAGGATGTGCGCGTCTCACAAACCACTTGGGACAGTGGCTT CTGCGCTGTCAACCCCAAGTTCATGGCTCTGATCTGTGAGGCCAGTGGGGGAGGGGCCTTCCTGGTGCTA CCCCTAGGCAAGACTGGACGAGTAGACAAGAACGTGCCCCTGGTCTGCGGCCACACTGCCCCTGTGCTAG ACATCGCCTGGTGTCCACACAATGACAATGTCATTGCCAGTGGCTCTGAGGACTGCACAGTTATGGTGTG GGAGATCCCGGATGGGGGCCTGGTGCTGCCCTTGCGGGAGCCTGTCGTCACCTTGGAGGGCCACACCAAG AGGGTTGGCATCGTGGCCTGGCACCCCACAGCCCAGAATGTGCTTCTCAGCGCAGGTTGTGACAATGTGA TCCTGGTGTGGGACGTGGGCACTGGGGCAGCTGTGCTGACGCTGGGCCCGGATGTGCACCCGGACACAAT CTACAGCGTGGACTGGAGCCGGGACGGTGCTCTCATCTGCACCTCCTGCCGTGACAAGCGAGTTCGTGTC ATCGAGCCTCGCAAAGGCACTGTTGTGGCTGAAAAGGACCGTCCCCATGAGGGGACTCGGCCAGTGCACG CTGTGTTTGTGTCAGAAGGGAAGATTCTAACCACGGGTTTCAGTCGCATGAGTGAGCGGCAGGTGGCGCT GTGGGACACAAAGCACCTGGAGGAGCCACTGTCCCTGCAGGAGCTGGACACAAGCAGCGGAGTCCTGCTA CCTTTTTTTGATCCTGATACCAACATTGTCTACCTCTGTGGCAAGGGTGACAGCTCTATCCGGTATTTTG AGATCACTTCTGAGGCTCCGTTCCTGCATTATCTTTCCATGTTCAGTTCCAAGGAGTCTCAGCGTGGTAT GGGCTACATGCCCAAACGTGGCCTGGAAGTGAATAAGTGTGAGATTGCCAGATTCTATAAGCTTCATGAG CGGAAGTGTGAGCCCATTGCCATGACAGTGCCTAGAAAGTCGGACCTGTTCCAGGAGGACCTGTATCCGC CCACTGCAGGACCCGACCCTGCCCTCACAGCTGAGGAATGGCTGGGTGGTCGGGATGCTGGCCCCCTCCT CATTTCCCTCAAGGATGGCTACGTGCCCCCAAAGAGCAGGGAGCTGAGGGTCAACAGGGGCCTGGACAGC GCTCGCAGAAGAGCTACACCAGAGCCCAGCGGCACGCCCAGCTCGGATACCGTGTCAAGGCTGGAGGAGG ACGTGAGGAATCTGAACGCCATAGTGCAAAAGCTACAAGAGCGCCTGGACAGGCTGGAGGAGACTGTTCA GGCCAAGTAGAACCCCAGAGCCCTCATCTGGGGCCAGCCATCCCCTCTGCTCCCACCCCACCCTCAGGCC ATATGGTGAATAAGACGAAAATAATAAAACGGCTTTATTTTCTGGTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3484185 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484185 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11205 same embryo
	EMAGE:11206 same embryo
	EMAGE:11209 same embryo
	EMAGE:11204 same embryo
	EMAGE:11208 same embryo
	EurExpress:euxassay_003404 same experiment
	MGI:4824011 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source