EMAGE mouse gene expression spatial database

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EMAGE:11236

Gcgr glucagon receptor ( MGI:99572)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001273. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001273_01	euxassay_001273_02	euxassay_001273_03	euxassay_001273_04

euxassay_001273_05	euxassay_001273_06	euxassay_001273_07	euxassay_001273_08	euxassay_001273_09

euxassay_001273_10	euxassay_001273_11	euxassay_001273_12	euxassay_001273_13	euxassay_001273_14

euxassay_001273_15	euxassay_001273_16	euxassay_001273_17	euxassay_001273_18	euxassay_001273_19

euxassay_001273_20	euxassay_001273_21	euxassay_001273_22	euxassay_001273_23	euxassay_001273_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11236_wholemount_strong.wlz

11236_wholemount_moderate.wlz

11236_wholemount_weak.wlz

11236_wholemount_possible.wlz

11236_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11236_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4897
Entity Detected:	Gcgr, glucagon receptor ( MGI:99572)
Sequence:	sense strand is shown >T4897 CGCGAGGAGCGCAGCCCTAGCCCCGGCGACTGAGCACACCTGAGGAGAGGTGCACACACTCTGAGGACCT AGGTGTGCAACCTCTGCCAGATGTGGGGCGTGGCTACCCAGAGGCATGCCCCTCACCCAGCTCCACTGTC CCCACCTGCTGCTGCTGCTGTTGGTGCTGTCATGTCTGCCAGAGGCACCCTCTGCCCAGGTAATGGACTT TTTGTTTGAGAAGTGGAAGCTCTATAGTGACCAATGCCACCACAACCTAAGCCTGCTGCCCCCACCTACT GAGCTGGTCTGTAACAGAACCTTCGACAAGTACTCCTGCTGGCCTGACACCCCTCCCAACACCACTGCCA ACATTTCCTGCCCCTGGTACCTACCTTGGTACCACAAAGTGCAGCACCGCCTAGTGTTCAAGAGGTGTGG GCCCGATGGGCAGTGGGTTCGAGGGCCACGGGGGCAGCCGTGGCGCAACGCCTCCCAATGTCAGTTGGAT GATGAAGAGATCGAGGTCCAGAAGGGGGTGGCCAAGATGTATAGCAGCCAGCAGGTGATGTACACCGTGG GCTACAGTCTGTCCCTGGGGGCCTTGCTCCTTGCGCTGGTCATCCTGCTGGGCCTCAGGAAGCTGCACTG CACCCGAAACTACATCCATGGGAACCTGTTTGCGTCCTTTGTGCTCAAGGCTGGCTCTGTGTTGGTCATC GATTGGCTGCTGAAGACACGGTACAGCCAGAAGATTGGCGATGACCTCAGTGTGAGCGTCTGGCTCAGTG ACGGGGCGATGGCCGGCTGCAGAGTGGCCACAGTGATCATGCAGTACGGCATCATAGCCAACTATTGCTG GTTGCTGGTAGAGGGCGTGTACCTGTACAGCCTGCTGAGCCTTGCCACCTTCTCTGAGAGGAGCTTCTTT TCCCTCTACCTGGGCATTGGCTGGGGTGCGCCCCTGCTGTTTGTCATCCCCTGGGTGGTGGTCAAGTGTC TGTTTGAGAATGTTCAGTGCTGGACCAGCAATGACAACATGGGATTCTGGTGGATCCTGCGTATTCCTGT CTTCCTGGCCTTACTGATCAATTTTTTCATCTTTGTCCACATCATTCACCTTCTTGTGGCCAAGCTGCGT GCCCATCAGATGCACTATGCTGACTATAAGTTCCGGCTGGCCAGGTCCACGCTGACCCTCATCCCTCTGC TGGGGGTCCACGAGGTGGTCTTTGCCTTTGTGACTGACGAGCATGCCCAAGGCACCCTGCGCTCCACCAA GCTCTTTTTTGACCTGTTCCTCAGCTCCTTCCAGGGTCTGCTGGTGGCTGTTCTCTACTGTTTCCTCAAC AAGGAGGTGCAGGCAGAGCTGATGCGGCGTTGGAGGCAATGGCAAGAAGGCAAAGCTCTTCAGGAGGAAA GGTTGGCCAGCAGCCATGGCAGCCACATGGCCCCAGCAGGGCCTTGTCATGGTGATCCCTGTGAGAAACT TCAGCTTATGAGTGCAGGCAGCAGCAGTGGGACTGGCTGTGTGCCCTCTATGGAGACCTCGCTGGCCAGT AGTCTCCCAAGGTTGGCTGACAGCCCCACCTGAATCTCCACTGGAGCCTAGCCAGGCTGCGTTCAGAAAG GGCCTCAGAGGACAACCCAGAGCCAGATGCCCGGCCAAGGCTGAAGAGACAAAGCAGCAAGACAGCAGCT TGTACTGTGCACACTCCCCTAACCTGTCCTAGCCTGGCACAGGCCACAGTGACAGAGTAGGGGTTGGATA TGATGGAGAAGCCATGTTATCTATGAACTCTGAGTGTTCCCATGTGTGTTGACATGGTCCCTGTACCCAG ATATGTCCTTCAGTAAAAAGCTCGAGTGGGAGCTGCTGCACAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5100574 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5100574 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11239 same embryo
	EMAGE:11237 same embryo
	EMAGE:11238 same embryo
	EMAGE:11235 same embryo
	EMAGE:11234 same embryo
	EurExpress:euxassay_001273 same experiment
	MGI:4825020 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source