EMAGE mouse gene expression spatial database

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EMAGE:11257

Gss glutathione synthetase ( MGI:95852)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006502. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006502_01	euxassay_006502_02	euxassay_006502_03	euxassay_006502_04

euxassay_006502_05	euxassay_006502_06	euxassay_006502_07	euxassay_006502_08	euxassay_006502_09

euxassay_006502_10	euxassay_006502_11	euxassay_006502_12	euxassay_006502_13	euxassay_006502_14

euxassay_006502_15	euxassay_006502_16	euxassay_006502_17	euxassay_006502_18	euxassay_006502_19

euxassay_006502_20	euxassay_006502_21	euxassay_006502_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11257_wholemount_strong.wlz

11257_wholemount_moderate.wlz

11257_wholemount_weak.wlz

11257_wholemount_possible.wlz

11257_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11257_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lens

strong

regional

strong expression: see section 01 02 03

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6583
Entity Detected:	Gss, glutathione synthetase ( MGI:95852)
Sequence:	sense strand is shown >T6583 CCCACGCGTCCGCAGCTGGACAACGAGCGAGTTGGGATGGCTACCAGCTGGGGCAGCATCTTGCAGGATG AGAAGCAGCTGGAAGAACTGGCAAAGCAGGCCATAGACCGGGCCCTGGCCGAGGGCGTGTTGCTGAGGTC CGCACAGCATCCCAGCTCCTCCGACGTGGTGACATATGCCCCATTCACGCTTTTCCCCTCGCCAGTACCC AGTGCTCTGCTGGAGCAGGCCTATGCTGTGCAGATGGACTTCAACATACTGGTGGATGCTGTCAGCCAGA ACCCAGCCTTCCTGGAGCAAACACTGTCTAGCACCATCAAAAAGGACGACTATACTGCCCGTCTCTTTGA TATCTACAAACAAGTCCTGAAAGAGGGCATTGCCCAGACCGTGTTCCTGGGCCTGAATCGCTCAGATTAC ATGTTCCAGTGCGGCGCAGACGGCTCCAAAGCCCTGAAACAGATCGAGATCAACACTATCTCTGCCAGCT TTGGGGGCCTGGCCTCCCGGACTCCAGCTGTGCACCGACACGTTCTCAATGTCCTGAATAAGACCAAAGA AGCTTCCAAGATCCTGTCCAATAACCCCAGCAAGGGACTGGCCCTGGGGATCGCCAAAGCCTGGGAGCTC TATGGCTCAGCTAATGCGGTGGTGCTACTGATTGCTCAAGAGAAGGAAAGGAACATATTTGACCAGCGTG CCGTAGAGAACGAGCTGCTAGACAGGAAGATCCATGTCATCCGTGGAAGATTTGAAGATGTCTCTGAAAG GGGTTCTCTGGACCAAAACCGAAGGCTGTTTATGGATGACCAGGAAGTTGCTGTGGTGTACTTCCGAGAT GGCTACATGCCCAGTCAGTATAATTCACAGAACTGGGAAGCACGCCTGATGCTAGAGAGATCTCGTGCTG CCAAGTGTCCAGACATTGCCATACAGCTGGCTGGGACTAAGAAGGTGCAGCAGGAACTGAGCAGGGTGGG TCTGCTGGAAGCACTGCTCCCGGGCCAGCCCGAGGCTGTGGCCCGCCTCCGAGCCACCTTTGCTGGCCTC TATTCACTGGACATGGGTGAAGAAGGGGACCAGGCCATTGCTGAGGCCCTTGCTGCTCCTAGCCACTTTG TGCTGAAGCCCCAGAGAGAGGGTGGAGGTAACAACTTATACGGGGAAGAAATGGTACAAGCTCTGGAGCA GCTGAAGGACAGTGAGGAGAGAGCCTCCTACATCCTCATGGAGAAGATTGAACCTGAGCCTTTTAGGAAT TGCTTGCTACGGCCTGGCAGCCCTGCCCAAGTGGTCCAGTGTATCTCGGAGCTGGGTATTTTTGGAGTCT ATGTCAGACAGGGAACAACACTGGTGATGAACAAGCATGTGGGGCACCTGCTTCGAACCAAAGCCGTGGA GCATGCAGACGGAGGTGTGGCGGCAGGAGTGGCAGTCCTGGACAACCCCTACCCTGTGTGAAGGCGCCAT CTGGACTTCACTCAGGAGGCCTTCTATCCCCTGTACTTGGCACTCCTCTTCTGAGGGGTTGCCCCTGTCC CTATCTTAGGGGAGCTTGTCTCTTCCATAGACCTCCAAAACTTCAGGGAAGGGAAAACCCAGGGTATCTT CCCTCAGCAGCCTTCCAGCCGAGGACCAGAAAAGCTATGATTCCATTAGAAGACTTCTGGAGGTCCCCAG ATCTTTGGAGTGTGGGAATGGAAGCTGCTTTGAGGCAAAGGCTCATAAACCCTGCAAGTCTTCATGGTCT TCTCACCAGCCTTTCCAGCAGGTTCTAGTGCCTTGACCTGGGGTAGGACCGAGTGAAGGAGGAAGAGGGT AAAAGGGCACAGACTTCCCCAGCTCTGCCCTAAATAAAATAACAATGCTGATTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3593913 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593913 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11255 same embryo
	EMAGE:11253 same embryo
	EMAGE:11254 same embryo
	EMAGE:11256 same embryo
	EurExpress:euxassay_006502 same experiment
	MGI:4825255 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source