EMAGE mouse gene expression spatial database

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EMAGE:11270

Brd3 bromodomain containing 3 ( MGI:1914632)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006625. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006625_01	euxassay_006625_02	euxassay_006625_03	euxassay_006625_04

euxassay_006625_05	euxassay_006625_06	euxassay_006625_07	euxassay_006625_08	euxassay_006625_09

euxassay_006625_10	euxassay_006625_11	euxassay_006625_12	euxassay_006625_13	euxassay_006625_14

euxassay_006625_15	euxassay_006625_16	euxassay_006625_17	euxassay_006625_18	euxassay_006625_19

euxassay_006625_20	euxassay_006625_21	euxassay_006625_22	euxassay_006625_23	euxassay_006625_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11270_wholemount_strong.wlz

11270_wholemount_moderate.wlz

11270_wholemount_weak.wlz

11270_wholemount_possible.wlz

11270_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11270_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7098
Entity Detected:	Brd3, bromodomain containing 3 ( MGI:1914632)
Sequence:	sense strand is shown >T7098 CCCACGCGTCCGCCCACGCGTCCGCGCCATCTTCCGAAGGGGACTGCCCGGAGGATGCGCGGCGCGGGAA GGTGCCTGATTGGGCCCAGAAATGGGATGCCAAGCCTCCACGAGGAGCGCCTCTTTGAGCTGCTGCTGTG ACAGCAAGTGACTAAGCTGCCAGAGGATGTCCACTACAGCGGCTGCCCCCACGGGGATCCCGGCAGTCCC GGGCCCCGTGAACCCTCCCCCACCTGAGGTCTCCAACCCCAGCAAGCCCGGGCGAAAGACTAACCAACTG CAGTACATGCAGAATGTAGTGGTGAAGACACTCTGGAAACATCAGTTTGCCTGGCCTTTCTACCAGCCTG TGGATGCAATCAAGCTGAACCTGCCTGATTATCATAAAATAATAAAAAACCCAATGGACATGGGGACTAT CAAGAAGAGACTAGAAAATAATTATTATTGGAGTGCCAGTGAGTGTATGCAGGACTTCAACACCATGTTT ACAAACTGTTATATTTATAATAAGCCCACAGATGATATAGTGCTAATGGCCCAGGCCTTAGAGAAAATCT TTCTGCAGAAAGTGGCCCAGATGCCTCAGGAGGAAGTTGAACTATTGCCCCCTGCTCCAAAGGGCAAAGG CCGGAAGCCAGCTGCAGGAGCCCAAAATGCAGGTTCCCAACAAGTGGCAGCTGTGTCTTCGGTCTCCCCA GCAACCCCCTTCCAGAACATACCCCCCACCGTGTCCCAGACACCCGTCATTGCCGCCACCCCTGTACCAA CCATCACTGCAAACGTCACGTCAGTTCCAGTCCCCCCACCCACTGCACCGCCTCCTCCTGCGACACCCAT CGTCCCTGTGGTCCCTCCCACACCGCCTGTAGTCAAGAAAAAGGGCGTGAAGCGGAAAGCAGACACAACC ACACCCACAACATCTGCCATCACTGCCAGCCGGAGTGAGTCTCCCCCGCCACTTTCAGAGCCCAAGCAAG CCAAGGTAGTGGCCCGAAGGGAGAGCGGGGGCCGCCCCATCAAACCTCCCAAGAAGGACCTGGAAGATGG TGAGGTCCCACAGCACGCGGGTAAAAAGGGAAAACTGTCCGAGCACCTGCGGCACTGTGACAGCATCCTC CGGGAGATGCTGTCCAAGAAGCATGCTGCCTATGCGTGGCCCTTTTACAAGCCAGTGGACGCGGAGGCGC TGGAGCTGCATGACTACCATGACATCATCAAGCACCCCATGGACCTCAGCACGGTCAAAAGGAAGATGGA TAGCCGAGAGTACCCAGATGCACAGGGCTTTGCTGCTGATATCCGGTTAATGTTCTCGAATTGTTATAAG TACAACCCTCCAGACCACGAAGTGGTGGCCATGGCCAGGAAGCTCCAGGATGTGTTTGAGATGAGGTTTG CCAAGATGCCCGATGAGCCCATGGAGGCACCTGCGCTGCCCGCTCCCACGGCCCCCATCGTGAGCAAGGG GGCTGAGAGCAGCCGCAGTAGTGAGGAGAGCTCTTCAGATTCAGGCAGCTCAGACTCAGAAGAGGAGCGA GCCACTCGGCTGGCTGAGCTGCAGGAGCAGCTGAAGGCCGTGCACGAACAGCTGGCTGCCTTGTCTCAGG CCCCAGTGAACAAACCAAAGAAGAAGAAAGAGAAGAAGGAAAAGGAAAAGAAGAAGAAAGACAAGGACAA AGACAAAGAGAAGGAGAAGCACAAGGCCAAGTCTGAGGAGGAGAAGAAGGCCAAGGCAGCCCCAGCAGCC AAGCAGGCCCAGCAGAAAAAGGCTCCCACCAAGAAGGCCAACAGCACAACCACAGCCAGCAGACAGCTCA AGAAGGGCGGCAAGCAGGCATCTGCGTCCTATGACTCAGAGGAAGAGGAGGAGGGCCTGCCCATGAGCTA TGATGAAAAGCGACAACTTAGCCTTGACATCAACCGGCTGCCCGGCGAGAAGCTAGGGCGTGTGGTGCAC ATCATTCAGTCTCGGGAGCCCTCGCTTCGGGACTCAAACCCAGACGAGATTGAGATTGACTTTGAGACCC TGAAGCCAACCACGCTGCGGGAACTGGAGAGATATGTCAAGTCTTGTTTACAAAAAAAGCAGAGGAAACC ATTGTCAACAAGCGGGAAGAAGCAGGCAGCCAAATCGAAAGAGGAGCTAGCTCAGGAGAAGAAAAAGGAG CTGGAGAAGCGGCTGCAGGATGTCAGTGGACAACTGAACAGCAAAAAACCCACTAAGAAAGAGAAGTCCG GCTCAGCTCCCTCAGGAGGCCCATCAAGGCTCAGCAGTAGCAGCTCCTCTGAGTCTGCGAGCAGTAGCTC CAGTGGATCGAGCTCCGACAGCAGTGACTCAGAGTGAACTCGGACTGGGACAGAGCTGGACAGATGGACG TGGTGCACGCCAACGCAGTGTCCCCTCCTATGGTTAATATACTTTCGTTCCATGGTGTGCAGGTTTTCTT TTACTTCAGTGTTATGATCTCTTCCAGCTGTGCTCCGGTAGGGCGGAGAGCCTCGCACGTGCACCAGACC CCATGAGAGGGCCGAGCTCTGCGAGGGGGAAATGCCTTTGCAGCTCCATTTAGTTGTTTGGAGACTGCTC CTGGGATGCTAACCTGATGGCCATAGACACGGTGGGCTGGAGGCGTGGCTGGACCTGCCCAATCCTTCCT CAGGACCAGCCTCTCCCTTAGTAACTTGAAGGAAGCTTTGTAAGGGTGTTTCTCTGGAATGGGTTCTGTG TTTGAAAGCTGTTAGAACACTAACCAGCAGGTTACTTCTCTCCGCAGGGGCTGAAGACAGATGTGTCAGC CCATGGGAACCAGCCTGGGCCCTAGTGGAGGGTCCTGGTAGCTGCAGACCAGAGCCTCACACCTCTGGGT TGGGTGTGGAGGAAGAGGGGTACTGAGTTCCCTGGTCCTTTGGCTGTGTGTGCAGCGTGCAGATTTCGGG ACACCGAAACTTTTACCTCAACTTAAGGGAATAAAAACAAACAAACAAACAAAAAAAGATTAAAAAAAAA ACAAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4015879 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4015879 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11269 same embryo
	EMAGE:11267 same embryo
	EMAGE:11268 same embryo
	EurExpress:euxassay_006625 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source