EMAGE mouse gene expression spatial database

EMAGE:11284

Abpg androgen binding protein gamma ( MGI:87864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006680. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006680_01	euxassay_006680_02	euxassay_006680_03	euxassay_006680_04

euxassay_006680_05	euxassay_006680_06	euxassay_006680_07	euxassay_006680_08	euxassay_006680_09

euxassay_006680_10	euxassay_006680_11	euxassay_006680_12	euxassay_006680_13	euxassay_006680_14

euxassay_006680_15	euxassay_006680_16	euxassay_006680_17	euxassay_006680_18	euxassay_006680_19

euxassay_006680_20	euxassay_006680_21	euxassay_006680_22	euxassay_006680_23	euxassay_006680_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midgut

strong

regional

strong expression: see section 16 moderate expression: see section 08 09 10 11 12 13 14 15 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7375
Entity Detected:	Abpg, androgen binding protein gamma ( MGI:87864)
Sequence:	sense strand is shown >T7375 CTCCACCATGAAGGGGACACTTCTTCTGCTGGCCTTGCTGGTGATTGGAGAGCTGGGCTTCCAGACAACG GAAGCATGCCTTTCTTTTGCCAGAACTTACGGAGCAATACTTACTCTAAGGAGGACCTTCCTGCATGGTG ACCTTTCACAGTTTTATGCTACTGTAGCTGAAAGGGTGGCCTTTGAAAAAATCCAGGATTGCTTTCGTGA GGAAGGACAAAAAACCATAATTCTGAATCCCCAAATTATGTTATCTCTATACTTAAGCCCAGAATGCAAG AAATACTATGGCAATGACCTCTTAAAGAAAATTCAAGATTTTCTTAACCAGTCAAATATCCATTAGAAGT TAATGGTTTATAGCCAACCCATCTTTGCAATGTGTCTGATCCAAGTGCCATCTTGCTGACCTGTCTTCTT TTTCTCCTGAAGTTGGATTCCTGACACCTGTGCCCTCATCCTGTACTCTCTTAGGCTGATTCTAATAAAT GCCTGCAGCTCTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4192901 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4192901 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11282 same embryo
	EMAGE:11283 same embryo
	EMAGE:11281 same embryo
	EurExpress:euxassay_006680 same experiment
	MGI:4822887 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source