EMAGE mouse gene expression spatial database

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EMAGE:11286

Slc41a3 solute carrier family 41, member 3 ( MGI:1918949)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006674. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006674_01	euxassay_006674_02	euxassay_006674_03	euxassay_006674_04

euxassay_006674_05	euxassay_006674_06	euxassay_006674_07	euxassay_006674_08	euxassay_006674_09

euxassay_006674_10	euxassay_006674_11	euxassay_006674_12	euxassay_006674_13	euxassay_006674_14

euxassay_006674_15	euxassay_006674_16	euxassay_006674_17	euxassay_006674_18	euxassay_006674_19

euxassay_006674_20	euxassay_006674_21	euxassay_006674_22	euxassay_006674_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11286_wholemount_strong.wlz

11286_wholemount_moderate.wlz

11286_wholemount_weak.wlz

11286_wholemount_possible.wlz

11286_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11286_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7065
Entity Detected:	Slc41a3, solute carrier family 41, member 3 ( MGI:1918949)
Sequence:	sense strand is shown >T7065 GCGCGCCTGGCCGCCTCGCTTTCGCCGCCGGCCGGGAGAGGACCGGTCAGGGCTGCTGGGCTCAGCCGTG TGCTGATGGAAGGGACAGAAGCCCGACAGAGGAGGCTGGAAGGCTGTGGAAGGCTGAAGGAGCTGGGGCC ATTGCCCAGCCACGATGCAGGAAGACTCCCAAAGGCTTCTGAAGAAGGACACCTGGCGGTCTCTGAGAGC CAACTCGTGGATGCCAAATCACTGGAGGCCCCGCCTGGCAGGGAGACGTCCCTGATCATTGGCTTTCAGG TGGTAATACCCTTCCTGCTTGCAGGCGTGGGACTGTCCTGGGCTGGCTTGCTTCTGAACTATTTCCAGCA CTGGCCAGTGTTCAAAGATGTTAAAGACCTGATGACGTTGGTGCCACCCCTCGTCGGTCTGAAGGGAAAC CTGGAAATGACGCTGGCGTCTCGGCTCTCCACGTCTGCCAACACTGGGCAAATCGATGACCGCCAGGAGA GGTACAAAATCATCAGCAGCAACCTCGCTGTGGTTCAGGTGCAGGCCACTGTTGTGGGGCTCCTGGCTGC AGTGGCCTCCCTGATGCTGGGCACAGTGTCCCACGAGGAGTTCGATTGGTCCAAGGTGGCCTTGCTGTGC ACGAGCAGCGTCATCACCGCCTTCCTTGCTGCCTTGGCCCTAGGAATTCTAATGATCTGCATAGTGATTG GTGCTCGGAAGTTTGGGGTCAACCCTGATAACATCGCCACACCCATTGCAGCCAGCCTGGGCGACCTCAT CACCCTGTCCATCCTGGCCCTCATGAGCAGTTTCTTCTACAGCCACAAAGACACATGGTATCTGACGCCT TTGGTCTGCGTCGGCTTCTTGGCTCTCACCCCTCTGTGGCTCTTCATTGCCAAGCAGAACCCGCCCATCA TGAAGATCCTGAAGTATGGGTGGTTTCCCATCATCCTGGCTATGATCATCAGCAGTTTCGGAGGTCTCAT CCTGAGCAAAACCATTTCGAAACATGAATTTAAAGGCATGGCTGTCCTAACTCCAGTCATGTGTGGTGTT GGTGGCAACCTGGTGGCCATTCAGACTAGCCGCATCTCCACCTTTCTGCATATGTGGAGCACACCCGGGG TCCTCCCTGTCCAGATGAAAAGATTCTGGCCTAACCCCTGCTTCATTTTCTGCTCTTCAGAAATCAATTC TGTGTCAGCACGAGTCCTGCTCTTTCTGGTGGTCCCAGGACACCTGATTTTCTTCTACCTCATCTGCCTG GTGGAAGGCCAGTCAGTGACAAACAGCAAGATCTTCATATTGCTGTACCTGGTGGCAGGCGTGGTCCAGG TGGTCATCCTGCTGTACCTGGCAGAAGTGACAGTTCGGCTGACATGGCACCAAGCCCTGGATCCTGACAA CCACTGCATCCCCTACCTCACGGGGCTGGGGGACCTGCTGGGCACTAGCCTGCTGGCACTGTGCTTCTTC CTGGACTGGTTACTGAGGGGCAGAGCTAATCTTCAGGAGCTGGTCTCAGAGCTGGTTTCTGTGCCTCCCT GACTGAGCCTACCACCCGCAGGTCTCCATGAAACGTTCCCTCATGTCCACAGTGGTCTGGCAAGCCGTTC ACCCCTGCTGATAGGCTTCAAGCTGCTAAACACCTGCGAGGGCAGTGGCCTCATCAGTCTGCCAGGGAGA CAAGTACACACCTTGACTCTCAAGTTGGCAGCCCAGCCTGAAGTAGAGCTCTGACATTAGGAGCTCTGCT TGCAAGTGTGTGAGTATGTATGCCATGGATGTATGCCTGGACTCTGTGTGTCTGTGTGTGTGTGTGTGTG TGTGTGTGTGTGTGTGTGTGTGTGTAGGGCAGGCTGTTCTAACCTGAAGATGCTGAGGAAGGAGCCTGTT GGTGCAACCTGGGTTGGTGATCCATTGGGTGGATCAGGACGGAACTCTGTGTCCCCAGGTCTCAGGAGTG CCATCCCCGGGCTCAGCTCCAGTTCCCTGGGCTCATGCTACTAGTGCTTTGGGCCCAGTGACCATGCACC TTCACCCCAGGCAGGCCCTGTATACCCCTCCTCTATACTCCAGCATTTAATCTTGTGGCTGCTTTCTTTT TATTTCCCACTTTCTCTGTTTTATAACAGAACCTTCAGTATAGGTTCTGGTAATCAAGAGATGTATAATA AAACATAGCTTATGTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4011836 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4011836 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11285 same embryo
	EMAGE:11287 same embryo
	EMAGE:11288 same embryo
	EurExpress:euxassay_006674 same experiment
	MGI:4828230 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source