EMAGE mouse gene expression spatial database

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EMAGE:11313

Hpd 4-hydroxyphenylpyruvic acid dioxygenase ( MGI:96213)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006640. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006640_01	euxassay_006640_02	euxassay_006640_03	euxassay_006640_04

euxassay_006640_05	euxassay_006640_06	euxassay_006640_07	euxassay_006640_08	euxassay_006640_09

euxassay_006640_10	euxassay_006640_11	euxassay_006640_12	euxassay_006640_13	euxassay_006640_14

euxassay_006640_15	euxassay_006640_16	euxassay_006640_17	euxassay_006640_18	euxassay_006640_19

euxassay_006640_20	euxassay_006640_21	euxassay_006640_22	euxassay_006640_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11313_wholemount_strong.wlz

11313_wholemount_moderate.wlz

11313_wholemount_weak.wlz

11313_wholemount_possible.wlz

11313_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11313_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7388
Entity Detected:	Hpd, 4-hydroxyphenylpyruvic acid dioxygenase ( MGI:96213)
Sequence:	sense strand is shown >T7388 CCACGCGTCCGCCCACGCGTCCGCACCATCAACCATGACAACCTACAACAACAAAGGACCAAAGCCTGAG AGAGGCCGGTTCCTCCATTTCCACTCGGTGACCTTCTGGGTTGGCAATGCCAAGCAGGCTGCTTCCTTCT ACTGCAACAAGATGGGCTTTGAACCTCTGGCCTACAGGGGCCTAGAGACTGGCTCCCGGGAGGTAGTCAG CCACGTCATCAAGCAAGGGAAAATTGTGTTTGTTCTCTGCTCTGCTCTCAATCCCTGGAACAAAGAGATG GGCGACCACTTGGTGAAGCATGGCGACGGGGTGAAAGACATCGCATTCGAGGTGGAAGACTGCGACCACA TTGTGCAGAAAGCTCGAGAACGGGGCGCCAAAATTGTGCGGGAGCCATGGGTGGAGCAAGACAAATTTGG GAAGGTGAAGTTTGCTGTGCTGCAGACGTATGGAGATACCACACACACCCTGGTGGAGAAGATCAACTAC ACTGGCCGTTTCTTACCTGGATTCGAGGCCCCAACATACAAGGATACCCTGCTTCCAAAACTACCCAGAT GTAACCTTGAGATCATTGACCACATTGTAGGCAACCAACCCGACCAAGAAATGCAGTCTGCCTCAGAATG GTACCTGAAAAACCTGCAGTTCCACCGGTTCTGGTCCGTGGACGACACGCAGGTGCACACGGAGTACAGC TCTCTGCGCTCCATTGTGGTGACCAACTACGAGGAATCCATCAAAATGCCCATCAACGAGCCAGCTCCGG GCAGGAAGAAGTCTCAGATCCAGGAATATGTGGACTATAATGGGGGTGCTGGGGTCCAGCACATCGCTCT CAAGACGGAAGACATCATCACAGCAATCCGCCACTTGAGGGAGCGAGGCACGGAGTTCTTGGCCGCCCCA TCTTCTTACTACAAACTGCTTCGGGAGAATCTCAAGTCAGCCAAGATCCAGGTGAAAGAGAGCATGGACG TCCTGGAGGAGCTGCATATCCTAGTCGACTATGACGAGAAAGGCTACCTCCTACAGATCTTCACCAAGCC CATGCAGGACCGGCCCACACTCTTCCTGGAAGTCATTCAACGTCACAACCACCAGGGCTTTGGAGCGGGC AACTTCAACTCTCTGTTCAAGGCGTTCGAGGAGGAGCAAGCCCTACGGGGCAACCTCACTGACCTGGAGC CCAATGGTGTGAGGTCTGGAATGTAAGCCCCCATCCACACATGGACCACGCAAAGTGCTGGACACATCAG TCATCTCCAACTGGCTGAAAGGCTGAACCTCAGGGCTCCACCCACGTCATGGCCACGCCCCCTCTATTAC AAGAGTCCGCCTTGCCTGAGTCCTCCCTGCTGAGTAAAGCTACCCTCCCAGGTCCAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4193670 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4193670 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11315 same embryo
	EMAGE:11318 same embryo
	EMAGE:11317 same embryo
	EMAGE:11314 same embryo
	EMAGE:11316 same embryo
	EurExpress:euxassay_006640 same experiment
	MGI:4825443 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source