EMAGE mouse gene expression spatial database

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EMAGE:11322

Fads3 fatty acid desaturase 3 ( MGI:1928740)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006636. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006636_01	euxassay_006636_02	euxassay_006636_03	euxassay_006636_04

euxassay_006636_05	euxassay_006636_06	euxassay_006636_07	euxassay_006636_08	euxassay_006636_09

euxassay_006636_10	euxassay_006636_11	euxassay_006636_12	euxassay_006636_13	euxassay_006636_14

euxassay_006636_15	euxassay_006636_16	euxassay_006636_17	euxassay_006636_18	euxassay_006636_19

euxassay_006636_20	euxassay_006636_21	euxassay_006636_22	euxassay_006636_23	euxassay_006636_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11322_wholemount_strong.wlz

11322_wholemount_moderate.wlz

11322_wholemount_weak.wlz

11322_wholemount_possible.wlz

11322_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11322_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14

pons mantle layer

moderate

single cell

moderate expression: see section 05 06 07 08 09 12 13 14 15

facial vii ganglion

moderate

regional

moderate expression: see section 02 03 04 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 06

ventral grey horn

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 weak expression: see section 06

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

clavicle

strong

regional

strong expression: see section 08 15 weak expression: see section 07 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7970
Entity Detected:	Fads3, fatty acid desaturase 3 ( MGI:1928740)
Sequence:	sense strand is shown >T7970 CCTCCCTCAGTGTCTCTCTTAGACCTTGGTCACGTACCGGGGTCCGGAGGACTTGTGTACAGCGGCAATG GGCGGTGTCGGGGAGCCCGGAGGGGGACCCGGGCCGCGGGAGGGGCCCGCACCGCTGGGGGCGCCCCTAC CCATCTTCCGCTGGGAGCAGATCCGCCAGCATGACCTACCAGGCGACAAGTGGCTGGTCATCGAGCGCCG TGTCTACGACATCAGCCGCTGGGCACAGCGGCACCCAGGGGGTAGCCGCCTCATCGGCCACCACGGTGCG GAGGACGCCACGGATGCCTTCCACGCCTTCCACCAAGATCTCCATTTTGTGCGCAAGTTCCTGAAACCCC TGTTGATTGGAGAGCTAGCCCCAGAGGAACCCAGCCAGGATGGAGCTCAGAATGCCCAGCTGATCGAGGA CTTCCGAGCCTTGCGCCAGGCAGCTGAAGACATGAAGCTGTTTGAAGCTGATACCACTTTCTTTGCACTC CTGCTGGGCCACATCCTGGCTATGGAGTTGTTGGCCTGGCTTATCATCTACCTCTTGGGCCCTGGCTGGG TGTCCAGTATCCTTGCTGCCCTGATCCTGGCCATCTCTCAGGCCCAGTGCTGGTGTCTGCAACATGATCT AGGTCATGCTTCCATCTTCACTAAGTCCAGGTGGAACCATGTGGCCCAGCAGTTCGTGATGGGGCAGTTG AAAGTGAGTGTGGAGCGGGGCGGGCATCTGTGCTGGGTCTGTTCAAGTGTGCGGGTACCAAGTTGGAGCC CAGCCTAACTCTGCCCCTGAGCTTCCTTCTCCCCACAGGGCTTTTCCGCCCACTGGTGGAATTTCCGCCA CTTCCAGCACCATGCCAAACCCAACATCTTCCACAAAGACCCAGATGTGACTGTCGCACCTGTCTTCCTC CTGGGGGAGTCATCTGTGGAGTATGGCAAGAAGAAACGCAGATACCTGCCCTACAACCACCAGCATCTAT ACTTCTTCCTGATTGGCCCTCCGCTGCTCACCTTGGTGAACTTTGAAGTTGAAAATCTGGCGTACATGCT GGTGTGCATGCAGTGGACGGACTTGCTGTGGGCTGCCAGTTTCTACTCCCGCTTTTTCTTGTCCTACTCT CCCTTCTATGGTGCCACTGGGACACTGCTCCTCTTTGTTGCTGTCAGGGTGCTGGAGAGCCACTGGTTCG TGTGGATCACGCAGATGAACCACATCCCCAAGGAGATTGGCCATGAAAAGCATCGGGACTGGGCAAGCTC TCAGCTGGCAGCCACCTGCAATGTGGAACCTTCGCTCTTCATTGACTGGTTCAGCGGGCACCTCAATTTC CAGATTGAGCACCACCTCTTCCCCACGATGCCAAGGCACAACTACCGGAGGGTGGCCCCCCTGGTCAAGG CGTTCTGCGCCAAGCACGGCCTACACTACGAGGTGAAGCCTTTCCTCACCGCTCTGGTGGATATCATCGG GTCCCTGAAGAAGTCTGGCGACATCTGGCTGGATGCATACCTCCATCAATGAAGGCAGCACCCTTATGGG TGGTGAAGGGGTAAGGGTCCAGACTCCAGCAGTCACCGAGTCACTGGGCAAGATTGACAGTCATCCTCAC CCCCTGCTGGGGCAGCCTGTCTGCCCGCCTGGTCCTGCTGGCTTTCCTCAGTCCTCAGCATTTTCTGGTC AGCAATCCTATGGCCATGGCACATGTCCAACAGGACCAGGGTGGAGGGAACATACTTAAAATGACACGGT GAGCATAGCATGTTTTCCTAGAGCAAGAACTGGAAGGAAAACTTGTTATTTTTATATAAAAAGCAACCCA GACACATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30291213 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30291213 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11320 same embryo
	EMAGE:11324 same embryo
	EMAGE:11323 same embryo
	EMAGE:11321 same embryo
	EMAGE:11319 same embryo
	EurExpress:euxassay_006636 same experiment
	MGI:4824680 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source