EMAGE mouse gene expression spatial database

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EMAGE:11396

Mical2 microtubule associated monoxygenase, calponin and LIM domain containing 2 ( MGI:2444947)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008567. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008567_01	euxassay_008567_02	euxassay_008567_03	euxassay_008567_04

euxassay_008567_05	euxassay_008567_06	euxassay_008567_07	euxassay_008567_08	euxassay_008567_09

euxassay_008567_10	euxassay_008567_11	euxassay_008567_12	euxassay_008567_13	euxassay_008567_14

euxassay_008567_15	euxassay_008567_16	euxassay_008567_17	euxassay_008567_18	euxassay_008567_19

euxassay_008567_20	euxassay_008567_21	euxassay_008567_22	euxassay_008567_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11396_wholemount_strong.wlz

11396_wholemount_moderate.wlz

11396_wholemount_weak.wlz

11396_wholemount_possible.wlz

11396_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11396_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

moderate

regional

moderate expression: see section 06 weak expression: see section 05 18

upper jaw molar

moderate

regional

moderate expression: see section 06 weak expression: see section 05 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35712
Entity Detected:	Mical2, microtubule associated monoxygenase, calponin and LIM domain containing 2 ( MGI:2444947)
Sequence:	sense strand is shown >T35712 GTTACCTCAGACAACCCCAGAGAACATCAACAAAAATTTTGAGCAGTACACATTGGACCCAGCCACGCGG TACCCAAACCTCAACCTGCACTGCGTCAGGCCTCACCAGGTGAAGCATTTGTACATCACTAAGGAGATGG ACCGCTTCCCTCTCGAGAGATGGGGCTCAGTGAGGAGATCTGTCAGCCTCTCCAGGCGGGAGTCAGACAT CCGGCCTAACAAGCTTTTAACCTGGTGCCAGCAGCAGACCAAGGGTTACCAGCACGTCAGAGTCACTGAC CTGACCACATCCTGGCGCAGCGGCTTGGCCCTGTGTGCCATCATCCACAGCTTCCGGCCAGAGCTGATCA ACTTTGACTCGCTGAATGAAGATGACGCTGTGGAGAACAACCAACTGGCATTTGATGTGGCCAAGCGTGA GTTTGGGATCCTGCCTGTGACCACAGGCAAAGAGATGGCATCTACCCAGGAGCCAGACAAGCTCAGCATG GTCATGTACCTCTCCAAGTTCTATGAGCTCTTCCGGGGCACTCCACTGAGACCCATGGATTCCTGGCGTA AAAACTATGGAGAAAATGCTGACTTTGGCTTGGGCAAAACATTCATTCAGAATAACTATCTCAACCTCAC ATTGCCCAGAAAGAGGACCCCACGGGTAGACACCCAGACTGAAGAGAATGACATGAACAAGAGGCGGCGA CAAGGCTTCAACCACCTGGAAGAGCTGCCATCCTTCTCCAGCCGCAGCCTGGGCTCCAGTCAAGAGTATG CTAAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89391. Forward Primer - name:089391_F_cDNA_5330438E18Rik, sequence:GTTACCTCAGACAACCCCAGAG; Reverse Primer - name:089391_N_SP6_cDNA_5330438E18Rik, sequence:TTTAGCATACTCTTGACTGGAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11391 same embryo
	EMAGE:11394 same embryo
	EMAGE:11392 same embryo
	EMAGE:11395 same embryo
	EMAGE:11393 same embryo
	EurExpress:euxassay_008567 same experiment
	MGI:4826196 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source