EMAGE mouse gene expression spatial database

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EMAGE:11450

Zfp87 zinc finger protein 87 ( MGI:107768)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006614. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006614_01	euxassay_006614_02	euxassay_006614_03	euxassay_006614_04

euxassay_006614_05	euxassay_006614_06	euxassay_006614_07	euxassay_006614_08	euxassay_006614_09

euxassay_006614_10	euxassay_006614_11	euxassay_006614_12	euxassay_006614_13	euxassay_006614_14

euxassay_006614_15	euxassay_006614_16	euxassay_006614_17	euxassay_006614_18	euxassay_006614_19

euxassay_006614_20	euxassay_006614_21	euxassay_006614_22	euxassay_006614_23	euxassay_006614_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11450_wholemount_strong.wlz

11450_wholemount_moderate.wlz

11450_wholemount_weak.wlz

11450_wholemount_possible.wlz

11450_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11450_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 05 22 23 24

kidney calyx

moderate

regional

moderate expression: see section 09 10 19 20 21 22

left lung

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12

right lung

weak

regional

weak expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7107
Entity Detected:	Zfp87, zinc finger protein 87 ( MGI:107768)
Sequence:	sense strand is shown >T7107 CCTAAGTTAGCGGTGCTGTGTCCTGCTCCTCGCTCTCGATCGGATCTCTGACATGGAGGAAATGCTGTCA TTCAGGGATGTGGCCATTGATTTCTCTGCAGAGGAGTGGGAATGCCTGGAGCCTGCTCAGTGGAATCTGT ACAGGGACGTGATGCTGGAGAATTACAGCCACCTTGTGTTCCTGGGTCTTGCATCCTGTAAGCCATACCT GGTCACATTTCTGGAGCAAAGACAAGAGCCTTCAGTTGTGAAGAGACCAGCAGCTGCCACCGTGCACCCA GGGGGGAAGCCCTATAAATGTCAAGAATTTGACAAGACCTTTGATTGGAACTCACTCCTTCTTCAGCGCC AGAGAGTTCATCCTGTGGAGAAGCCCTACAAATGTGAAGAATGTGGGAAGGTCTTTAGTGCTCACTCGGC ACTTTCTATTCACCAGCGAATCCACACCGGAGAGAAACCCTACAAGTGTGAAGAGTGTGGCAAGGCCTTC AGTACTCGCTCAGCACTTTATATACACAAGAAAATCCACTCTGGAGACAAACCCTACAAGTGTGAGGAGT GTGCCAAAGCCTTTTATTTCCCTTCATTGCTTAAGCAACATCAACGAACTCATTCTGCAGAGAATCTCTG CAAGTGTGAGGAGTGTGGCAAAGCCTTTTATTTACCTTCATTCCTTAAGCAACATCAAAGAATTCATTCT GCAGAGAATCCTTACAGGTGTGAAGAATGTGGGAAAGCCTTTTCCTATCCCTCAACCCTAAAGCAGCATC AAAGAATTCATTCTGGAGAGAAACCATACCGTTGTGAAGAATGTGGCAAAGCCTTCCGTAGGTCAACATA CTTTCACCAACACCAAAGAATTCATACTGGAGAGAAACCCTACCAGTGTGAAGAGTGTGGGAAAACATTC TACTATCGTTCAAACCTGAAGGGACATCAGATTATTCATTCTGGAGTGAAACCCTACAAGTGTGAAGTAT GTGGGAGCATGTTTAGAACTAGCTCAGACCTTTCTAAGCACCAGAGGATCCATGCGGAAGTGAAGCTGTA CAGTTGTGAGGAGTGCGGGAAAGCCTTTTCTACTCATTCGTACCTCATTCGGCACAAATTGGGCCACTCT AGAGAGAAACCATACAAATGCGAAGAGTGTGGCAAAACATTTTGTTATCCTTCAATCCTTAAGGAACATC AACGAATTCATTCTGGAGTGAAACCTTACAATTGTGAAATATGTGGCAGCATGTTTAGAACTCGCTCCGA ACTTTCTAAGCACCAGAGAATCCATGCTGAAGTGAAGCTCTACAACTGTGAGGAGTGCGGGAAAGCCTTT TCTACTCACTCCTACCTTATGAGGCATAGACTTGGTCACTCTGGAGAGAAGCCCTACAAATGTGAAGAGT GTGGGAAATCCTATAGCTACCCTTCACTCCTTAAGGAACATCAAAGAATTCATTCTCAGAATCCCTATAA GTGTGACGTGTGTGGGAAGGTTTTTTGTACTCGCTCAGGACGTTCTAAACACCAGAGAATTCACACCGGA GAGAAACCTTACAAGTGTGAAGACTGTGGGAAGGGATTCTCGACTCACTCATACCTTACTCTGCACAAAT TACTTCATACCGGAGAGAAACCTTTCAAATGGGAAGAATGTGGTGAGGTTTTCTATACCTGCTGAAGACT TACTAAACATCAGGAAATTCACATTGGCGAAAACCCTACAAAGGTAAAGAATGTGTCAAAACTCTTTACT GTACTTCAGACTTTAAATAATACCATAAAAACTCATACTGAAGAGAAACCATACTTTAATTAGGGCTTCG TTAGATACAAGGTACGTCCTACTGTAGAGAAACTTTACAGATGTGGAGAATGTGGACAAATATTTTACCG TACTTCACAACTTTAACAGCCAGCAAAAGGGCTCATTCTCAGAAGAATCCCTTCAAGTGTGAAGTATGGG ACAAGGTATTATGTGCTACCTTGAGCTTTCTGTCTACCAGAACATGTATCCTGGAGAGAAACTCTGCAAG TGTGAAAAATTTGGAAAGTTTTTTAACTCATTCCTCCCTGATTCTGATCAGACTTGGCAAAGTTTGGGGC GGGCGTTGTGTTTTTGTTTTAGGATAATTGTGCTCAGGCCTGTTGATTGTGGGAGGAACCAACCCATCTC TGTTGAGCAAGGATCCTGGATTGTATAAAAGGAGAAAGGGAACTGAGAAGAAGCGCTCCTCTCTCTTCCT CTGCCCTCTGACTGTCGAGGCAATAGGAACTTCTCTTTCAGCTCCCGCTGCCCTAAAATGTCTCACGACG TTCACAGGGTCGTGATTCGAATGAGAAATGTTAACCCTGTAGTCTTAAGCATTTGAACATTTGTGCCCCG AGTTGTTGGCTCTATTTGGAGAGATTTAGAAGTAGGTCACTAGAGGTGAACTTTGTGAATAAAGAGCTCC CTCTACATCCAGTTCTCTCACTCTGCTTCCTGAGTACCATTGAGGATGTGAACTCTCAGCTTCCGGCTTC GGCCACCATGCAGCTTATTCCCATGCCCACCCTCTGTCGTGAACTTCGATCCCTTCTGTACCGCAAACTG AAGTAAAGTCATTCCTCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4017149 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4017149 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11447 same embryo
	EMAGE:11446 same embryo
	EMAGE:11449 same embryo
	EMAGE:11448 same embryo
	EurExpress:euxassay_006614 same experiment
	MGI:4829344 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source