EMAGE mouse gene expression spatial database

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EMAGE:11460

Clpb ClpB caseinolytic peptidase B homolog (E. coli) ( MGI:1100517)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006929. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006929_01	euxassay_006929_02	euxassay_006929_03	euxassay_006929_04

euxassay_006929_05	euxassay_006929_06	euxassay_006929_07	euxassay_006929_08	euxassay_006929_09

euxassay_006929_10	euxassay_006929_11	euxassay_006929_12	euxassay_006929_13	euxassay_006929_14

euxassay_006929_15	euxassay_006929_16	euxassay_006929_17	euxassay_006929_18	euxassay_006929_19

euxassay_006929_20	euxassay_006929_21	euxassay_006929_22	euxassay_006929_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11460_wholemount_strong.wlz

11460_wholemount_moderate.wlz

11460_wholemount_weak.wlz

11460_wholemount_possible.wlz

11460_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11460_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 11 12 16 17

cervical ganglion

strong

regional

strong expression: see section 09 10 11 17 18

thoracic ganglion

strong

regional

strong expression: see section 13 14 15 16

stomach proventricular region

strong

regional

strong expression: see section 03 04 05 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9132
Entity Detected:	Clpb, ClpB caseinolytic peptidase B homolog (E. coli) ( MGI:1100517)
Sequence:	sense strand is shown >T9132 CTAGCACAGGGGCCGGCACCAGCGGGTTTTAGAAGCAGGTGTCATGATGTTGTCCGCGGTGTTGAGGAGA ACCACGCCAGCGCCTCGGCTCTTCCTAGGGTTAATCAAATCTCCATCTCTCCAAAGCCGGGGAGGTGCTT ACGGCCGTGGCGTTGTCACCGGAGACCGCGGGGAGCCGCAGCGGTTGAGAGCTGCCGCTTGGGTGCGCCC TGGAGCATCTTCTACCTTCGTCCCCGGACGGGGGGCAGCCACCTGGGGGCGCCGTGGAGAACGCACCGAG ATCCCATACCTGACTGCCGCCTCGTCGGAACGCGGTCCTAGCCCCGAAGAAACACTCCCGGGACAAGACA GCTGGAATGGGGTCCCCAACAAGACGGGTCTGGGCATGTGGGCCTTGGCCATGGCGCTGGTGGTTCAGTG CTACAGCAAGAATCCATCCAACAAGGATGCAGCTCTAATGGAAGCTGCCCGGGCCAATAACGTGCAGGAA GTCAGAAGGCTGTTATCAGAAGGTGCAGATGTCAATGCAAGACACAAACTTGGCTGGACAGCACTGATGG TGGCATCCATCAGCCACAATGAGAGTGTGGTGCAGGTCCTGCTTGCTGCTGGAGCTGACCCAAACCTTGG GGATGAGTTCAGCAGTGTCTACAAGACTGCCAATGAACAGGGAGTCCATTCTCTGGAAGTCCTAGTCACT CGAGAGGATGACTTCAACAACCGGCTAAATCACCGTGCTAGCTTCAAAGGCTGCACAGCCCTGCACTATG CTGTCCTGGCCGATGACTACAGCATCGTCAAGGAGCTGCTGGACAGAGGAGCCAACCCCCTTCAGAGGAA TGAGATGGGACACACACCCCTGGATTATGCCCGGGAAGGGGAGGTTATGAAGCTTCTGAAAACTTCTGAG ACCAAGTACATGGAGAAGCAGAGGAAGCGGGAGGCAGAGGAGCGGCGCCGCTTCCCACTGGAGCAGCGAC TGAAGGAGCACATCATTGGCCAGGAGAGTGCCATCGCCACCGTCGGTGCTGCGATCCGGAGGAAGGAGAA CGGCTGGTACGATGAGGAGCACCCTCTAGTCTTCCTCTTCTTGGGATCGTCTGGAATAGGGAAAACCGAG CTGGCCAAGCAGACAGCAAAGTATATGCACAAAGATGCCAAAAAGGGCTTCATCCGGCTCGACATGTCTG AGTTCCAGGAGCGACATGAGGTAGCCAAGTTTATCGGTTCTCCACCAGGCTACATCGGCCATGAAGAGGG TGGCCAGCTGACCAAGAAACTGAAACAGTGCCCAAATGCAGTAGTCCTCTTTGATGAGGTGGACAAGGCC CATCCAGATGTGCTCACCATCATGCTGCAGCTGTTTGATGAGGGCCGCCTGACAGATGGCAAAGGGAAGA CCATTGACTGCAAGGATGCCATCTTCATCATGACTTCCAATGTAGCCAGTGATGAGATCGCCCAGCATGC ATTGCAGCTCAGACAGGAAGCTTTGGAGATGAGCCGAAATCGAATCGCTGAAAACCTTGGGGATGTCCAG ATGAGTGACAAGATCACCATCTCCAAGAACTTTAAAGAGAATGTGATCCGACCCATCTTGAAAGCTCACT TCCGGAGAGACGAGTTTCTGGGACGGATCAATGAGATCGTCTACTTCCTCCCTTTCTGCCACTCAGAGCT CATCCAGCTGGTCAACAAGGAACTGAACTTCTGGGCCAAGAGAGCCAAGCAGAGGCACAACATCACACTA CTGTGGGACCGCGAGGTGGCAGATGTGTTGGTTGATGGCTACAACGTCCACTATGGTGCCCGCTCCATTA AGCATGAGGTAGAGCGGCGTGTGGTAAACCAACTGGCGGCAGCCTATGAGCAGGACCTGCTACCAGGGGG TTGCACCCTGCGTATCACTGTGGAGGATTCGGACAAGCACCTCCTCAAGAGCCCTGAGCTGCCCTCACCC CAGGCTGAGAAGCGTCCACCTACATTGCGACTGGAGATCATTGATAAGGACAGCAAGACCCGCAAACTGG ACATCCAGGCACCACTCCACCCTGAGAAGGTGTGCTACACCATCTAGCATCCACCTGTCTGTGCCCTGAA CATCTAATAAAGGCCCCTTGCTGTGGCATGGCAACCAATCCACCTTGCCCTCATGCCTTTCCAAGGCCTA GCACCCTCTCCTGCCTTTCTTGGTTGTGAGGTTCATAGTCTGTTATGATCTCCAAGGCAAGGAACAGGAA GCCTGGTCTGCACTTCAGTGTGCACTCCACTCCATGTCCTCACTAGAGCATTGACGTGTGGCCAGAAGCT CAGAAACATGCAGCGGAGAACAAGTCTGGCTGAAGAAGATAGGACTCAGGCAGTGGTGCACCACCTCTGC TTGTGCTGATGCGGCTCATCCTCAGGATGGCCCCGTGGCAGTGGGGGGAAGCCAGGAGCCCCAAGCACAG GTAGCTCTGTAGTTTATTCTACAGCTACCTCTTTCCCATGCCCTACAACTGTCAGAGTCAAGGCTTGTGA CTGAGCCAGTGTAGAGCACCCATAGGTCTGACTGGTCCCTCTTCCTGCCTTCCTCTGGGGTGGGACCATG GTCCCAGGTGAACACCTCAGCTCTGAGATGGGGCTCATTCAGCAGGCTGGCAGTCTAGATAACAGAAGGT GCTGTGGGAGCAAGGCTGGAGTCAGGCTCCACACAGGTGGGCACGGGTCTGCCTTAGCCCTGTCTCTTAA CTGCCGTCCCAGCCAGGTAGCAATTCCATGGGCCTAGCATACCCATAAAGGACAGCAGGACACAGCAGGG CCTGGCAAGTCACTCTGCATCATCTGCAGAAGCCATCACCACTACAGGACTGAGTAGAGCCCTCCTGTGT GTTAGCCAGCCTCTGTCAAGATGTTTAGACAGGGCTGAAGTCAGTGCCATGACTCATAGATTGTCATGAC AAGATGCTCACAGCTCTGAGTGACACCACCTCACCCCTAAGAGGCTGCCTGCTCCTTACTACCTCAAAAC TGTATCCTCTTTTTACTTTGAAATTCTCTAGGCTTACAGAAAATTATGAAGAAAAAAAAAAGTAAACCTC TTATACCTTTAACTCAGCCTCTCCTGTCACACAGCATAGACGCATGGGGAGTGACAGGACCAGGAAAGTA GCATTGCCACACACTCCTGCCCCATCTGCAGGCTGTGGCTTTCACCAGCCTGGACATCCCACAGTGCATT CTACTTTCCATCGCCTTCCCCTGTGTCGCCCAAGGCAGTTTCTGTGTCTTCCCTGCTCTTCGGTGATCGT GACACTTTTGAAGAGTCCAGGCCAGTTCTTTTGTAGAATATCCTTCAGCCGAGCTCTATCTAATGCTCTC ACTGATGAGTCTGGGGGTCTGCGTTTTTGGCAAGGTGACCACAGAGACCAATGCCCTGCTGCCTCCCTCA CAGCACGTGGCAGTAACCCCAAAGCTCGAATTGTCTGGAAAAAGGTCCCCTACTGTAAAATTGAAAGTTT CACGCTGGGGTGTAGTATTAGACCTTGGCTAGCATGCACAGAGGCCCGGGTTGATTCCCAGTGCCACAAA AATAAATAAGTGTATAAATATAATTTTTTCCTAGAACTAAAATCAAGCCAGACTACTTACTGGTTTGTGC CTGGACTCCCAGCAATTGGGAGGTTGAGGTAGGATTGTCACAAGTTTGAGGCTACTTTGTGCTGCATACT AAGTTCCAGGCCAGCCTAGGCTACATAGTAAAACCCAATCTCCAAGAGTCAAGTGGATACATCTCTATAA AGTCGAGACTGGCCTGGTCTGCAGAGAGAGCTGCGGGCCAGCCAGGACTGCAGAGTGAGACCTTGTCTTA AAAAACAGCAATCCTTTACCATGGCCCAGCAGGTTATGGCACTTTTTACCCAGCCTGATGACTTGAGTTA ATTCCTTGAACCCATATAAAAATGAAGGTAGAGAACGGACCTCAAAAAAAAAAATGGTTCTCTGACCACA CACGTGGTGACATGACTTCCTGCCCCACACAGACATATACATGACAGTAATAATACTTTTAAAGTAATGA TAATGTTCTGGGAAGATACTTAAAGCTGTGTAAATGCCACGTGCTTATATTTTTTAGAGTGAGTGTCCAT CTCAGCTGAGACATTTTACTGTTGAATTTCCTGTTTTACTTGTGCAGATTTGTTGTTCCTTATCCATCTG TGTTTAATAATTGGTGTTCTGTTCCAAAGAAACCTTTCACCTCATTCATTTACTCAGAATGTATGCCCAT GTATTCTGTGGTTATGATGTAGTAGGAAGTGTGTTGCTCCGTGCAGCCACGTGGGGCCTATGTGAGCTCC TTCATGCTTTGGCGTTGCTTGCACCTCCATGTTCTGCCACTGTAGACTGTCCTGGGCCAACTCACAGCCT CCACACCACAGCTCCCATTTCTCCAAGGCCTTCCTGTTTCCTGTTATTGTGTGAACCAATGGCTTCTTGA TGCTCTAAAGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825472 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825472 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11461 same embryo
	EMAGE:11459 same embryo
	EMAGE:11462 same embryo
	EurExpress:euxassay_006929 same experiment
	MGI:4823934 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source