EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11487

Bcl2a1a B-cell leukemia/lymphoma 2 related protein A1a ( MGI:102687)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006845. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006845_01	euxassay_006845_02	euxassay_006845_03	euxassay_006845_04

euxassay_006845_05	euxassay_006845_06	euxassay_006845_07	euxassay_006845_08	euxassay_006845_09

euxassay_006845_10	euxassay_006845_11	euxassay_006845_12	euxassay_006845_13	euxassay_006845_14

euxassay_006845_15	euxassay_006845_16	euxassay_006845_17	euxassay_006845_18	euxassay_006845_19

euxassay_006845_20	euxassay_006845_21	euxassay_006845_22	euxassay_006845_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11487_wholemount_strong.wlz

11487_wholemount_moderate.wlz

11487_wholemount_weak.wlz

11487_wholemount_possible.wlz

11487_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11487_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

single cell

moderate expression: see section 11 12 14 15 weak expression: see section 13 16

mandible

moderate

single cell

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23

maxilla

moderate

single cell

moderate expression: see section 04 05 06 07 09 10 11 12 15 16 17 18 19 20 21 22 23 weak expression: see section 08

left lung

moderate

single cell

moderate expression: see section 08 09 10 11 weak expression: see section 03 04 05 06 07 12 13

right lung

moderate

single cell

moderate expression: see section 17 18 19 20 21 22 23 weak expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8214
Entity Detected:	Bcl2a1a, B-cell leukemia/lymphoma 2 related protein A1a ( MGI:102687)
Sequence:	sense strand is shown >T8214 CCTGACAAGACCTATCTTTGCAGTCTTTGCCTCCTTGGCCTCTAGGTGGACAGCTGCAGTCAGCTCTGCC AGGGAAGATGGCTGAGTACGAGTTCATGTATATCCACTCCCTGGCTGAGCACTATCTTCAGTATGTGCTA CAGGTACCCGCCTTTGAGTCGGCTCCAAGCCAAGCATGCAGAGTGCTACAAAGAGTTGCTTTCTCCGTTC AGAAGGAAGTTGAAAAGAATCTGAAGTCATACTTGGATGACTTTCACGTGGAATCCATAGATACCGCCAG AATAATATTCAACCAAGTGATGGAAAAAGAGTTTGAAGATGGCATCATTAACTGGGGAAGGATTGTGACT ATATTTGCCTTTGGGGGTGTTCTCCTCAAAAAACTTCCGCAAGAGCAGATTGCCCTGGATGTAGGTGCTT ACAAACAAGTTTCCAGTTTTGTGGCAGAATTCATAATCAATAACACAGGAGAATGGATACGGCGGAATGG AGGTTGGGAAGATGGCTTCATAAAGAAGTTTGAACCCAAATCTGGCTGGCTGACTTTTCTGCAGATGACA GGACAGATCTGGGAAATGCTCTTTCTCCTCAAGTAACACTACGGACCAGAGAGAACCTTGGCTCCCACGA GAATGGTTATCAGCACTTGGCATTTCCTTTTACAAACATGAAACTTGTCTTTCCAGAGTTGCAGATATTT TGTTTCAACTTTAATGAATAAATTACTAGTATTTGTTTCTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1226745 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1226745 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11485 same assay
	EurExpress:euxassay_006845 same experiment
	EMAGE:11484 same embryo
	EMAGE:11486 same embryo
	EMAGE:31831 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source